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The Arabidopsis thaliana checkpoint kinase WEE1 protects against premature vascular differentiation during replication stress

Toon Cools (UGent) , Anelia Iantcheva, Annika K Weimer, Shannah Boens, Naoki Takahashi (UGent) , Sara Maes (UGent) , Hilde Van Den Daele (UGent) , Gert Van Isterdael (UGent) , Arp Schnittger and Lieven De Veylder (UGent)
(2011) PLANT CELL. 23(4). p.1435-1448
Author
Organization
Abstract
A sessile lifestyle forces plants to respond promptly to factors that affect their genomic integrity. Therefore, plants have developed checkpoint mechanisms to arrest cell cycle progression upon the occurrence of DNA stress, allowing the DNA to be repaired before onset of division. Previously, the WEE1 kinase had been demonstrated to be essential for delaying progression through the cell cycle in the presence of replication-inhibitory drugs, such as hydroxyurea. To understand the severe growth arrest of WEE1-deficient plants treated with hydroxyurea, a transcriptomics analysis was performed, indicating prolonged S-phase duration. A role for WEE1 during S phase was substantiated by its specific accumulation in replicating nuclei that suffered from DNA stress. Besides an extended replication phase, WEE1 knockout plants accumulated dead cells that were associated with premature vascular differentiation. Correspondingly, plants without functional WEE1 ectopically expressed the vascular differentiation marker VND7, and their vascular development was aberrant. We conclude that the growth arrest of WEE1-deficient plants is due to an extended cell cycle duration in combination with a premature onset of vascular cell differentiation. The latter implies that the plant WEE1 kinase acquired an indirect developmental function that is important for meristem maintenance upon replication stress.
Keywords
PLANT DEVELOPMENT, CELL-DIVISION, IONIZING-RADIATION, GENE-EXPRESSION, DNA-DAMAGE CHECKPOINT, CYCLIN-DEPENDENT KINASE, S-PHASE CHECKPOINT, PROGRESSION, FORKS, PHOSPHATASE

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MLA
Cools, Toon, et al. “The Arabidopsis Thaliana Checkpoint Kinase WEE1 Protects against Premature Vascular Differentiation during Replication Stress.” PLANT CELL, vol. 23, no. 4, 2011, pp. 1435–48, doi:10.1105/tpc.110.082768.
APA
Cools, T., Iantcheva, A., Weimer, A. K., Boens, S., Takahashi, N., Maes, S., … De Veylder, L. (2011). The Arabidopsis thaliana checkpoint kinase WEE1 protects against premature vascular differentiation during replication stress. PLANT CELL, 23(4), 1435–1448. https://doi.org/10.1105/tpc.110.082768
Chicago author-date
Cools, Toon, Anelia Iantcheva, Annika K Weimer, Shannah Boens, Naoki Takahashi, Sara Maes, Hilde Van Den Daele, Gert Van Isterdael, Arp Schnittger, and Lieven De Veylder. 2011. “The Arabidopsis Thaliana Checkpoint Kinase WEE1 Protects against Premature Vascular Differentiation during Replication Stress.” PLANT CELL 23 (4): 1435–48. https://doi.org/10.1105/tpc.110.082768.
Chicago author-date (all authors)
Cools, Toon, Anelia Iantcheva, Annika K Weimer, Shannah Boens, Naoki Takahashi, Sara Maes, Hilde Van Den Daele, Gert Van Isterdael, Arp Schnittger, and Lieven De Veylder. 2011. “The Arabidopsis Thaliana Checkpoint Kinase WEE1 Protects against Premature Vascular Differentiation during Replication Stress.” PLANT CELL 23 (4): 1435–1448. doi:10.1105/tpc.110.082768.
Vancouver
1.
Cools T, Iantcheva A, Weimer AK, Boens S, Takahashi N, Maes S, et al. The Arabidopsis thaliana checkpoint kinase WEE1 protects against premature vascular differentiation during replication stress. PLANT CELL. 2011;23(4):1435–48.
IEEE
[1]
T. Cools et al., “The Arabidopsis thaliana checkpoint kinase WEE1 protects against premature vascular differentiation during replication stress,” PLANT CELL, vol. 23, no. 4, pp. 1435–1448, 2011.
@article{1266381,
  abstract     = {{A sessile lifestyle forces plants to respond promptly to factors that affect their genomic integrity. Therefore, plants have developed checkpoint mechanisms to arrest cell cycle progression upon the occurrence of DNA stress, allowing the DNA to be repaired before onset of division. Previously, the WEE1 kinase had been demonstrated to be essential for delaying progression through the cell cycle in the presence of replication-inhibitory drugs, such as hydroxyurea. To understand the severe growth arrest of WEE1-deficient plants treated with hydroxyurea, a transcriptomics analysis was performed, indicating prolonged S-phase duration. A role for WEE1 during S phase was substantiated by its specific accumulation in replicating nuclei that suffered from DNA stress. Besides an extended replication phase, WEE1 knockout plants accumulated dead cells that were associated with premature vascular differentiation. Correspondingly, plants without functional WEE1 ectopically expressed the vascular differentiation marker VND7, and their vascular development was aberrant. We conclude that the growth arrest of WEE1-deficient plants is due to an extended cell cycle duration in combination with a premature onset of vascular cell differentiation. The latter implies that the plant WEE1 kinase acquired an indirect developmental function that is important for meristem maintenance upon replication stress.}},
  author       = {{Cools, Toon and Iantcheva, Anelia and Weimer, Annika K and Boens, Shannah and Takahashi, Naoki and Maes, Sara and Van Den Daele, Hilde and Van Isterdael, Gert and Schnittger, Arp and De Veylder, Lieven}},
  issn         = {{1040-4651}},
  journal      = {{PLANT CELL}},
  keywords     = {{PLANT DEVELOPMENT,CELL-DIVISION,IONIZING-RADIATION,GENE-EXPRESSION,DNA-DAMAGE CHECKPOINT,CYCLIN-DEPENDENT KINASE,S-PHASE CHECKPOINT,PROGRESSION,FORKS,PHOSPHATASE}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{1435--1448}},
  title        = {{The Arabidopsis thaliana checkpoint kinase WEE1 protects against premature vascular differentiation during replication stress}},
  url          = {{http://doi.org/10.1105/tpc.110.082768}},
  volume       = {{23}},
  year         = {{2011}},
}
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