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Obligate biotrophy features unraveled by the genomic analysis of rust fungi

Sébastien Duplessis, Christina A Cuomo, Yao-Cheng Lin UGent, Andrea Aerts, Emilie Tisserant, Claire Veneault-Fourrey, David L Joly, Stéphane Hacquard, Joëlle Amselem and Brandi L Cantarel, et al. (2011) PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 108(22). p.9166-9171
abstract
Rust fungi are some of the most devastating pathogens of crop plants. They are obligate biotrophs, which extract nutrients only from living plant tissues and cannot grow apart from their hosts. Their lifestyle has slowed the dissection of molecular mechanisms underlying host invasion and avoidance or suppression of plant innate immunity. We sequenced the 101-Mb genome of Melampsora larici-populina, the causal agent of poplar leaf rust, and the 89-Mb genome of Puccinia graminis f. sp. tritici, the causal agent of wheat and barley stem rust. We then compared the 16,399 predicted proteins of M. larici-populina with the 17,773 predicted proteins of P. graminis f. sp tritici. Genomic features related to their obligate biotrophic lifestyle include expanded lineage-specific gene families, a large repertoire of effector-like small secreted proteins, impaired nitrogen and sulfur assimilation pathways, and expanded families of amino acid and oligopeptide membrane transporters. The dramatic up-regulation of transcripts coding for small secreted proteins, secreted hydrolytic enzymes, and transporters in planta suggests that they play a role in host infection and nutrient acquisition. Some of these genomic hallmarks are mirrored in the genomes of other microbial eukaryotes that have independently evolved to infect plants, indicating convergent adaptation to a biotrophic existence inside plant cells.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SYMBIOSIS, EVOLUTION, SEQUENCES, HOST, INSIGHTS, RESISTANCE, HAUSTORIA, evolution, rust disease, SMUT FUNGI, plant pathogen, basidiomycete, Comparative genomics, POPLAR
journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Proc. Natl. Acad. Sci. USA
volume
108
issue
22
pages
9166 - 9171
Web of Science type
Article
Web of Science id
000291106200053
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
9.681 (2011)
JCR rank
3/54 (2011)
JCR quartile
1 (2011)
ISSN
0027-8424
DOI
10.1073/pnas.1019315108
project
Bioinformatics: from nucleotids to networks (N2N)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1266320
handle
http://hdl.handle.net/1854/LU-1266320
date created
2011-06-16 16:15:31
date last changed
2013-02-27 09:11:18
@article{1266320,
  abstract     = {Rust fungi are some of the most devastating pathogens of crop plants. They are obligate biotrophs, which extract nutrients only from living plant tissues and cannot grow apart from their hosts. Their lifestyle has slowed the dissection of molecular mechanisms underlying host invasion and avoidance or suppression of plant innate immunity. We sequenced the 101-Mb genome of Melampsora larici-populina, the causal agent of poplar leaf rust, and the 89-Mb genome of Puccinia graminis f. sp. tritici, the causal agent of wheat and barley stem rust. We then compared the 16,399 predicted proteins of M. larici-populina with the 17,773 predicted proteins of P. graminis f. sp tritici. Genomic features related to their obligate biotrophic lifestyle include expanded lineage-specific gene families, a large repertoire of effector-like small secreted proteins, impaired nitrogen and sulfur assimilation pathways, and expanded families of amino acid and oligopeptide membrane transporters. The dramatic up-regulation of transcripts coding for small secreted proteins, secreted hydrolytic enzymes, and transporters in planta suggests that they play a role in host infection and nutrient acquisition. Some of these genomic hallmarks are mirrored in the genomes of other microbial eukaryotes that have independently evolved to infect plants, indicating convergent adaptation to a biotrophic existence inside plant cells.},
  author       = {Duplessis, S{\'e}bastien and Cuomo, Christina A and Lin, Yao-Cheng and Aerts, Andrea and Tisserant, Emilie and Veneault-Fourrey, Claire and Joly, David L and Hacquard, St{\'e}phane and Amselem, Jo{\"e}lle and Cantarel, Brandi L and Chiu, Readman and Coutinho, Pedro M and Feau, Nicolas and Field, Matthew and Frey, Pascal and Gelhaye, Eric and Goldberg, Jonathan and Grabherr, Mandred G and Kodira, Chinnappa D and Kohler, Annegret and K{\"u}es, Ursula and Lindquist, Erika A and Lucas, Susan M and Mago, Rohit and Mauceli, Evan and Morin, Emmanuelle and Murat, Claude and Pangilinan, Jasmyn L and Park, Robert and Pearson, Matthew and Quesneville, Hadi and Rouhier, Nicolas and Sakthikumar, Sharadha and Salamov, Asaf A and Schmutz, Jeremy and Selles, Benjamin and Shapiro, Harris and Tanguay, Philippe and Tuskan, Gerald A and Henrissat, Bernard and Van de Peer, Yves and Rouz{\'e}, Pierre and Ellis, Jeffrey G and Dodds, Peter N and Schein, Jacqueline E and Zhong, Shaobin and Hamelin, Richard C and Grigoriev, Igor V and Szabo, Les J and Martin, Francis},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keyword      = {SYMBIOSIS,EVOLUTION,SEQUENCES,HOST,INSIGHTS,RESISTANCE,HAUSTORIA,evolution,rust disease,SMUT FUNGI,plant pathogen,basidiomycete,Comparative genomics,POPLAR},
  language     = {eng},
  number       = {22},
  pages        = {9166--9171},
  title        = {Obligate biotrophy features unraveled by the genomic analysis of rust fungi},
  url          = {http://dx.doi.org/10.1073/pnas.1019315108},
  volume       = {108},
  year         = {2011},
}

Chicago
Duplessis, Sébastien, Christina A Cuomo, Yao-Cheng Lin, Andrea Aerts, Emilie Tisserant, Claire Veneault-Fourrey, David L Joly, et al. 2011. “Obligate Biotrophy Features Unraveled by the Genomic Analysis of Rust Fungi.” Proceedings of the National Academy of Sciences of the United States of America 108 (22): 9166–9171.
APA
Duplessis, S., Cuomo, C. A., Lin, Y.-C., Aerts, A., Tisserant, E., Veneault-Fourrey, C., Joly, D. L., et al. (2011). Obligate biotrophy features unraveled by the genomic analysis of rust fungi. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 108(22), 9166–9171.
Vancouver
1.
Duplessis S, Cuomo CA, Lin Y-C, Aerts A, Tisserant E, Veneault-Fourrey C, et al. Obligate biotrophy features unraveled by the genomic analysis of rust fungi. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2011;108(22):9166–71.
MLA
Duplessis, Sébastien, Christina A Cuomo, Yao-Cheng Lin, et al. “Obligate Biotrophy Features Unraveled by the Genomic Analysis of Rust Fungi.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 108.22 (2011): 9166–9171. Print.