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Measurement of porto-systemic shunting in mice by novel three-dimensional micro-single photon emission computed tomography imaging enabling longitudinal follow-up

Christophe Van Steenkiste UGent, Steven Staelens UGent, Steven Deleye UGent, Filip De Vos UGent, Stefaan Vandenberghe UGent, Anja Geerts UGent, Christophe Van De Wiele UGent, MARTINE DE VOS UGent, Hans Van Vlierberghe UGent and Isabelle Colle UGent (2010) LIVER INTERNATIONAL. 30(8). p.1211-1220
abstract
Background and aims: The reference method for diagnosing porto-systemic shunting (PSS) in experimental portal hypertension involves measuring 51Chrome (51Cr)-labelled microspheres. Unfortunately, this technique necessitates the sacrifice of animals. Alternatively, 99mtechnetium-macroaggregated albumin (99mTc-MAA) has been used; however, planar scintigraphy imaging techniques are not quantitatively accurate and adequate spatial information is not attained. Here, we describe a reliable, minimally invasive and rapid in vivo imaging technique, using three-dimensional single photon emission computed tomography (3D SPECT) modus, that allows more accurate quantification, serial measurements and spatial discrimination. Methodology: Partial portal vein ligation, common bile duct ligation and sham were induced in male mice. A mixture of 51Cr microspheres and 99mTc-macroaggregated albumin particles was injected into the splenic pulpa. All mice were scanned in vivo with mu SPECT (1 mm spatial resolution) and, when mandatory for localisation, a mu SPECT-CT was acquired. A relative quantitative analysis was performed based on the 3D reconstructed datasets. Additionally, 51Cr was measured in the same animals to calculate the correlation coefficient between the 99mTc detection and the gold standard 51Cr. In each measuring modality, the PSS fraction was calculated using the formula: [(lung counts)/(lung counts+liver counts)] x 100. Results: A significant correlation between the 99mTc detection and 51Cr was demonstrated in partial portal vein ligation, common bile duct ligation and sham mice and there was a good agreement between the two modalities. mu SPECT scanning delivers high spatial resolution and 3D image reconstructions. Conclusion: We have demonstrated that quantitative high-resolution mu SPECT imaging with 99mTc-MAA is useful for detecting the extent of PSS in a non-sacrificing set-up. This technology permits serial measurements and high-throughput screening to detect baseline PSS, which is especially important in pharmacological studies.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SPLANCHNIC BLOOD-FLOW, 99mtechnetium, SPECT, porto-systemic shunting, cirrhosis, 51chrome, BILE-DUCT LIGATION, HYPERTENSIVE-RATS, CIRCULATION, MODELS, SPIRONOLACTONE, ANGIOGENESIS, SORAFENIB
journal title
LIVER INTERNATIONAL
Liver Int.
volume
30
issue
8
pages
1211 - 1220
Web of Science type
Article
Web of Science id
000280666800019
JCR category
GASTROENTEROLOGY & HEPATOLOGY
JCR impact factor
3.84 (2010)
JCR rank
16/71 (2010)
JCR quartile
1 (2010)
ISSN
1478-3223
DOI
10.1111/j.1478-3231.2010.02276.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1261993
handle
http://hdl.handle.net/1854/LU-1261993
date created
2011-06-14 14:24:26
date last changed
2011-06-15 11:22:26
@article{1261993,
  abstract     = {Background and aims: The reference method for diagnosing porto-systemic shunting (PSS) in experimental portal hypertension involves measuring 51Chrome (51Cr)-labelled microspheres. Unfortunately, this technique necessitates the sacrifice of animals. Alternatively, 99mtechnetium-macroaggregated albumin (99mTc-MAA) has been used; however, planar scintigraphy imaging techniques are not quantitatively accurate and adequate spatial information is not attained. Here, we describe a reliable, minimally invasive and rapid in vivo imaging technique, using three-dimensional single photon emission computed tomography (3D SPECT) modus, that allows more accurate quantification, serial measurements and spatial discrimination.
Methodology: Partial portal vein ligation, common bile duct ligation and sham were induced in male mice. A mixture of 51Cr microspheres and 99mTc-macroaggregated albumin particles was injected into the splenic pulpa. All mice were scanned in vivo with mu SPECT (1 mm spatial resolution) and, when mandatory for localisation, a mu SPECT-CT was acquired. A relative quantitative analysis was performed based on the 3D reconstructed datasets. Additionally, 51Cr was measured in the same animals to calculate the correlation coefficient between the 99mTc detection and the gold standard 51Cr. In each measuring modality, the PSS fraction was calculated using the formula: [(lung counts)/(lung counts+liver counts)] x 100.
Results: A significant correlation between the 99mTc detection and 51Cr was demonstrated in partial portal vein ligation, common bile duct ligation and sham mice and there was a good agreement between the two modalities. mu SPECT scanning delivers high spatial resolution and 3D image reconstructions.
Conclusion: We have demonstrated that quantitative high-resolution mu SPECT imaging with 99mTc-MAA is useful for detecting the extent of PSS in a non-sacrificing set-up. This technology permits serial measurements and high-throughput screening to detect baseline PSS, which is especially important in pharmacological studies.},
  author       = {Van Steenkiste, Christophe and Staelens, Steven and Deleye, Steven and De Vos, Filip and Vandenberghe, Stefaan and Geerts, Anja and Van De Wiele, Christophe and DE VOS, MARTINE and Van Vlierberghe, Hans and Colle, Isabelle},
  issn         = {1478-3223},
  journal      = {LIVER INTERNATIONAL},
  keyword      = {SPLANCHNIC BLOOD-FLOW,99mtechnetium,SPECT,porto-systemic shunting,cirrhosis,51chrome,BILE-DUCT LIGATION,HYPERTENSIVE-RATS,CIRCULATION,MODELS,SPIRONOLACTONE,ANGIOGENESIS,SORAFENIB},
  language     = {eng},
  number       = {8},
  pages        = {1211--1220},
  title        = {Measurement of porto-systemic shunting in mice by novel three-dimensional micro-single photon emission computed tomography imaging enabling longitudinal follow-up},
  url          = {http://dx.doi.org/10.1111/j.1478-3231.2010.02276.x},
  volume       = {30},
  year         = {2010},
}

Chicago
Van Steenkiste, Christophe, Steven Staelens, Steven Deleye, Filip De Vos, Stefaan Vandenberghe, Anja Geerts, Christophe Van De Wiele, MARTINE DE VOS, Hans Van Vlierberghe, and Isabelle Colle. 2010. “Measurement of Porto-systemic Shunting in Mice by Novel Three-dimensional Micro-single Photon Emission Computed Tomography Imaging Enabling Longitudinal Follow-up.” Liver International 30 (8): 1211–1220.
APA
Van Steenkiste, C., Staelens, S., Deleye, S., De Vos, F., Vandenberghe, S., Geerts, A., Van De Wiele, C., et al. (2010). Measurement of porto-systemic shunting in mice by novel three-dimensional micro-single photon emission computed tomography imaging enabling longitudinal follow-up. LIVER INTERNATIONAL, 30(8), 1211–1220.
Vancouver
1.
Van Steenkiste C, Staelens S, Deleye S, De Vos F, Vandenberghe S, Geerts A, et al. Measurement of porto-systemic shunting in mice by novel three-dimensional micro-single photon emission computed tomography imaging enabling longitudinal follow-up. LIVER INTERNATIONAL. 2010;30(8):1211–20.
MLA
Van Steenkiste, Christophe, Steven Staelens, Steven Deleye, et al. “Measurement of Porto-systemic Shunting in Mice by Novel Three-dimensional Micro-single Photon Emission Computed Tomography Imaging Enabling Longitudinal Follow-up.” LIVER INTERNATIONAL 30.8 (2010): 1211–1220. Print.