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Steinernema everestense n. sp. (Rhabditida: Steinernematidae), a new species of entomopathogenic nematode from Pakhribas, Dhankuta, Nepal

Hari Bahadur Khatri-Chhetri, Lieven Waeyenberge, Sergei Spiridonov, Hira Kaji Manandhar and Maurice Moens UGent (2011) NEMATOLOGY. 13(4). p.443-462
abstract
A new species of entomopathogenic nematode, Steinernema everestense n. sp., was recovered from soil samples collected from Pakhribas, Dhankuta district of Nepal, during a survey in 2007. The analysis of ITS-rDNA and D2D3 LSU sequences placed S. everestense n. sp. in the feltiae-kraussei-oregonense group. The nematode can be separated from other described species of Steinernema by morphological and morphometric characteristics and by characterisations and phylogeny inferred from DNA sequences of the D2D3 LSU or ITS regions of rDNA. For infective juveniles, the new species can be recognised by the body length of 775 (705-838) μm, pharynx 119 (110-130) μm long, H% = 61 (47-68) and E% = 78 (60-89). The lateral field pattern is 2, 8, 6, 4, 3. The males have well curved, brownish-yellow spicules with a prominent but short velum, gubernaculum plump, boat-shaped to almost straight in lateral view, cuneus long, needle-shaped, corpus with two wings in ventral view. Spicule and gubernaculum length of the first generation males is 79 (65-87) μm and 50 (39-57) μm, respectively. A tail mucron is present in both male generations. Genital papillae total 23 or 25, comprising 11 or 12 pairs and a single midventral papilla. Specimens with 23 genital papillae have eight precloacal pairs (seven pairs subventral and one pair lateral), two pairs subterminal and one pair subdorsal. The most posterior two pairs are consistently located near the tail tip. The specimens with 25 genital papillae have an extra adcloacal pair of papillae and the anteriormost two pairs are close together. The vulva is slightly protruding, mostly with equal lips, and low epiptygmata. A post-anal swelling is developed in most first and all second generation females.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
molecular, description, SEM, taxonomy, phylogeny, morphometrics, new species, morphology, CHINA, PROVINCE, USA
journal title
NEMATOLOGY
Nematology
volume
13
issue
4
pages
443 - 462
Web of Science type
Article
Web of Science id
000291565600005
JCR category
ZOOLOGY
JCR impact factor
0.911 (2011)
JCR rank
86/146 (2011)
JCR quartile
3 (2011)
ISSN
1388-5545
DOI
10.1162/138855410X526859
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
1261016
handle
http://hdl.handle.net/1854/LU-1261016
date created
2011-06-14 10:53:12
date last changed
2012-04-27 15:15:58
@article{1261016,
  abstract     = {A new species of entomopathogenic nematode, Steinernema everestense n. sp., was recovered from soil samples collected from Pakhribas, Dhankuta district of Nepal, during a survey in 2007. The analysis of ITS-rDNA and D2D3 LSU sequences placed S. everestense n. sp. in the feltiae-kraussei-oregonense group. The nematode can be separated from other described species of Steinernema by morphological and morphometric characteristics and by characterisations and phylogeny inferred from DNA sequences of the D2D3 LSU or ITS regions of rDNA. For infective juveniles, the new species can be recognised by the body length of 775 (705-838) \ensuremath{\mu}m, pharynx 119 (110-130) \ensuremath{\mu}m long, H\% = 61 (47-68) and E\% = 78 (60-89). The lateral field pattern is 2, 8, 6, 4, 3. The males have well curved, brownish-yellow spicules with a prominent but short velum, gubernaculum plump, boat-shaped to almost straight in lateral view, cuneus long, needle-shaped, corpus with two wings in ventral view. Spicule and gubernaculum length of the first generation males is 79 (65-87) \ensuremath{\mu}m and 50 (39-57) \ensuremath{\mu}m, respectively. A tail mucron is present in both male generations. Genital papillae total 23 or 25, comprising 11 or 12 pairs and a single midventral papilla. Specimens with 23 genital papillae have eight precloacal pairs (seven pairs subventral and one pair lateral), two pairs subterminal and one pair subdorsal. The most posterior two pairs are consistently located near the tail tip. The specimens with 25 genital papillae have an extra adcloacal pair of papillae and the anteriormost two pairs are close together. The vulva is slightly protruding, mostly with equal lips, and low epiptygmata. A post-anal swelling is developed in most first and all second generation females.},
  author       = {Khatri-Chhetri, Hari Bahadur and Waeyenberge, Lieven and Spiridonov, Sergei  and Manandhar, Hira Kaji  and Moens, Maurice},
  issn         = {1388-5545},
  journal      = {NEMATOLOGY},
  keyword      = {molecular,description,SEM,taxonomy,phylogeny,morphometrics,new species,morphology,CHINA,PROVINCE,USA},
  language     = {eng},
  number       = {4},
  pages        = {443--462},
  title        = {Steinernema everestense n. sp. (Rhabditida: Steinernematidae), a new species of entomopathogenic nematode from Pakhribas, Dhankuta, Nepal},
  url          = {http://dx.doi.org/10.1162/138855410X526859},
  volume       = {13},
  year         = {2011},
}

Chicago
Khatri-Chhetri, Hari Bahadur, Lieven Waeyenberge, Sergei Spiridonov, Hira Kaji Manandhar, and Maurice Moens. 2011. “Steinernema Everestense N. Sp. (Rhabditida: Steinernematidae), a New Species of Entomopathogenic Nematode from Pakhribas, Dhankuta, Nepal.” Nematology 13 (4): 443–462.
APA
Khatri-Chhetri, H. B., Waeyenberge, L., Spiridonov, S., Manandhar, H. K., & Moens, M. (2011). Steinernema everestense n. sp. (Rhabditida: Steinernematidae), a new species of entomopathogenic nematode from Pakhribas, Dhankuta, Nepal. NEMATOLOGY, 13(4), 443–462.
Vancouver
1.
Khatri-Chhetri HB, Waeyenberge L, Spiridonov S, Manandhar HK, Moens M. Steinernema everestense n. sp. (Rhabditida: Steinernematidae), a new species of entomopathogenic nematode from Pakhribas, Dhankuta, Nepal. NEMATOLOGY. 2011;13(4):443–62.
MLA
Khatri-Chhetri, Hari Bahadur, Lieven Waeyenberge, Sergei Spiridonov, et al. “Steinernema Everestense N. Sp. (Rhabditida: Steinernematidae), a New Species of Entomopathogenic Nematode from Pakhribas, Dhankuta, Nepal.” NEMATOLOGY 13.4 (2011): 443–462. Print.