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Identification of new polymorphic microsatellite markers in the NA1 and NA2 lineages of Phytophthora ramorum

(2011) MYCOLOGIA. 103(6). p.1245-1249
Author
Organization
Abstract
Phytophthora ramorum is a recently introduced pathogen in Europe and North America consisting of three clonal lineages. Due to the very limited intra-lineage genetic variation, only a few polymorphic markers are available for use in studies involving the epidemiology and evolution of P. ramorum. A total of 159 primer pairs for candidate polymorphic SSR loci were tested with universal labeling. Four polymorphic microsatellite loci were identified within the NA1 lineage and one within the NA2 lineage, demonstrating the power and flexibility of the screening technique. The markers may significantly increase the number of genotypes that can be identified, and as such can help better characterize the North American lineages of P. ramorum.
Keywords
NURSERY, MIGRATION, CALIFORNIA, NORTH-AMERICA, CLONAL LINEAGES, OAK DEATH PATHOGEN, Sudden Oak Death, oomycetes, microsatellite markers, genetic diversity, universal labeling

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Chicago
Vercauteren, Annelies, Meg Larsen, Erica Goss, Niklaus J Grünwald, Martine Maes, and Kurt Heungens. 2011. “Identification of New Polymorphic Microsatellite Markers in the NA1 and NA2 Lineages of Phytophthora Ramorum.” Mycologia 103 (6): 1245–1249.
APA
Vercauteren, Annelies, Larsen, M., Goss, E., Grünwald, N. J., Maes, M., & Heungens, K. (2011). Identification of new polymorphic microsatellite markers in the NA1 and NA2 lineages of Phytophthora ramorum. MYCOLOGIA, 103(6), 1245–1249.
Vancouver
1.
Vercauteren A, Larsen M, Goss E, Grünwald NJ, Maes M, Heungens K. Identification of new polymorphic microsatellite markers in the NA1 and NA2 lineages of Phytophthora ramorum. MYCOLOGIA. 2011;103(6):1245–9.
MLA
Vercauteren, Annelies, Meg Larsen, Erica Goss, et al. “Identification of New Polymorphic Microsatellite Markers in the NA1 and NA2 Lineages of Phytophthora Ramorum.” MYCOLOGIA 103.6 (2011): 1245–1249. Print.
@article{1260524,
  abstract     = {Phytophthora ramorum is a recently introduced pathogen in Europe and North America consisting of three clonal lineages. Due to the very limited intra-lineage genetic variation, only a few polymorphic markers are available for use in studies involving the epidemiology and evolution of P. ramorum. A total of 159 primer pairs for candidate polymorphic SSR loci were tested with universal labeling. Four polymorphic microsatellite loci were identified within the NA1 lineage and one within the NA2 lineage, demonstrating the power and flexibility of the screening technique. The markers may significantly increase the number of genotypes that can be identified, and as such can help better characterize the North American lineages of P. ramorum.},
  author       = {Vercauteren, Annelies and Larsen, Meg and Goss, Erica  and Gr{\"u}nwald, Niklaus J and Maes, Martine and Heungens, Kurt},
  issn         = {0027-5514},
  journal      = {MYCOLOGIA},
  language     = {eng},
  number       = {6},
  pages        = {1245--1249},
  title        = {Identification of new polymorphic microsatellite markers in the NA1 and NA2 lineages of Phytophthora ramorum},
  url          = {http://dx.doi.org/10.3852/10-420},
  volume       = {103},
  year         = {2011},
}

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