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Susceptibility of in vitro produced hatched bovine blastocysts to infection with bluetongue virus serotype 8

Leen Vandaele UGent, Wendy Wesselingh UGent, Kris De Clercq, Ilse De Leeuw, Herman Favoreel UGent, Ann Van Soom UGent and Hans Nauwynck UGent (2011) VETERINARY RESEARCH. 42.
abstract
Bluetongue virus serotype 8 (BTV-8), which caused an epidemic in ruminants in central Western Europe in 2006 and 2007, seems to differ from other bluetongue serotypes in that it can spread transplacentally and has been associated with an increased incidence of abortion and other reproductive problems. For these reasons, and also because BTV-8 is threatening to spread to other parts of the world, there is a need for more information on the consequences of infection during pregnancy. The aim of the present study was to investigate whether hatched (i.e. zona pellucida-free) in vitro produced bovine blastocysts at 8-9 days post insemination are susceptible to BTV-8 and whether such infection induces cell death as indicated by apoptosis. Exposure of hatched in vitro produced bovine blastocysts for 1 h to a medium containing 10(3.8) or 10(4.9) TCID50 of the virus resulted in active viral replication in between 25 and 100% of the cells at 72 h post exposure. The infected blastocysts also showed growth arrest as evidenced by lower total cell numbers and a significant level of cellular apoptosis. We conclude from this in vitro study that some of the reproductive problems that are reported when cattle herds are infected with BTV-8 may be attributed to direct infection of blastocysts and other early-stage embryos in utero.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
APOPTOSIS, EXPRESSION, FIBRONECTIN, IMPLANTATION, CATTLE, PREIMPLANTATION EMBRYOS, PROTEINS, BELGIUM, CELLS, SHEEP
journal title
VETERINARY RESEARCH
Vet. Res.
volume
42
article_number
14
pages
8 pages
Web of Science type
Article
Web of Science id
000290659600003
JCR category
VETERINARY SCIENCES
JCR impact factor
4.06 (2011)
JCR rank
1/141 (2011)
JCR quartile
1 (2011)
ISSN
0928-4249
DOI
10.1186/1297-9716-42-14
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
1253727
handle
http://hdl.handle.net/1854/LU-1253727
date created
2011-06-06 15:07:41
date last changed
2011-06-15 11:30:37
@article{1253727,
  abstract     = {Bluetongue virus serotype 8 (BTV-8), which caused an epidemic in ruminants in central Western Europe in 2006 and 2007, seems to differ from other bluetongue serotypes in that it can spread transplacentally and has been associated with an increased incidence of abortion and other reproductive problems. For these reasons, and also because BTV-8 is threatening to spread to other parts of the world, there is a need for more information on the consequences of infection during pregnancy. The aim of the present study was to investigate whether hatched (i.e. zona pellucida-free) in vitro produced bovine blastocysts at 8-9 days post insemination are susceptible to BTV-8 and whether such infection induces cell death as indicated by apoptosis. Exposure of hatched in vitro produced bovine blastocysts for 1 h to a medium containing 10(3.8) or 10(4.9) TCID50 of the virus resulted in active viral replication in between 25 and 100\% of the cells at 72 h post exposure. The infected blastocysts also showed growth arrest as evidenced by lower total cell numbers and a significant level of cellular apoptosis. We conclude from this in vitro study that some of the reproductive problems that are reported when cattle herds are infected with BTV-8 may be attributed to direct infection of blastocysts and other early-stage embryos in utero.},
  articleno    = {14},
  author       = {Vandaele, Leen and Wesselingh, Wendy and De Clercq, Kris and De Leeuw, Ilse and Favoreel, Herman and Van Soom, Ann and Nauwynck, Hans},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {APOPTOSIS,EXPRESSION,FIBRONECTIN,IMPLANTATION,CATTLE,PREIMPLANTATION EMBRYOS,PROTEINS,BELGIUM,CELLS,SHEEP},
  language     = {eng},
  pages        = {8},
  title        = {Susceptibility of in vitro produced hatched bovine blastocysts to infection with bluetongue virus serotype 8},
  url          = {http://dx.doi.org/10.1186/1297-9716-42-14},
  volume       = {42},
  year         = {2011},
}

Chicago
Vandaele, Leen, Wendy Wesselingh, Kris De Clercq, Ilse De Leeuw, Herman Favoreel, Ann Van Soom, and Hans Nauwynck. 2011. “Susceptibility of in Vitro Produced Hatched Bovine Blastocysts to Infection with Bluetongue Virus Serotype 8.” Veterinary Research 42.
APA
Vandaele, L., Wesselingh, W., De Clercq, K., De Leeuw, I., Favoreel, H., Van Soom, A., & Nauwynck, H. (2011). Susceptibility of in vitro produced hatched bovine blastocysts to infection with bluetongue virus serotype 8. VETERINARY RESEARCH, 42.
Vancouver
1.
Vandaele L, Wesselingh W, De Clercq K, De Leeuw I, Favoreel H, Van Soom A, et al. Susceptibility of in vitro produced hatched bovine blastocysts to infection with bluetongue virus serotype 8. VETERINARY RESEARCH. 2011;42.
MLA
Vandaele, Leen, Wendy Wesselingh, Kris De Clercq, et al. “Susceptibility of in Vitro Produced Hatched Bovine Blastocysts to Infection with Bluetongue Virus Serotype 8.” VETERINARY RESEARCH 42 (2011): n. pag. Print.