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Modifications in lignin and accumulation of phenolic glucosides in poplar xylem upon down-regulation of caffeoyl-coenzyme A O-methyltransferase, an enzyme involved in lignin biosynthesis

(2000) JOURNAL OF BIOLOGICAL CHEMISTRY. 275(47). p.36899-36909
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Abstract
Caffeoyl-coenzyme A O-methyltransferase (CCoAOMT) methylates, in vitro, caffeoyl-CoA and 5-hydroxyferuloyl-CoA, two possible precursors in monolignol biosynthesis in vivo. To clarify the in vivo role of CCoAOMT in lignin biosynthesis, transgenic poplars with 10% residual CCoAOMT protein levels in the stem xylem were generated. Upon analysis of the xylem, the affected transgenic lines had a 12% reduced Klason lignin content, an 11% increased syringyl/guaiacyl ratio in the noncondensed lignin fraction, and an increase in lignin-attached p-hydroxybenzoate but otherwise a lignin composition similar to that of wild type. Stem xylem of the CCoAOMT-down-regulated lines had a pink-red coloration, which coincided with an enhanced fluorescence of mature vessel cell walls. The reduced production of CCoAOMT caused an accumulation of O-3-beta -D-glucopyranosyl-caffeic acid, O-4-beta -D-glucopyranosyl-vanillic acid, and O-4-beta -D-glucopyranosyl-sinapic acid (GSA), as authenticated by H-1 NMR Feeding experiments showed that O-3-beta -D-glucopyranosyl-caffeic acid and GSA are storage or detoxification products of caffeic and sinapic acid, respectively. The observation that down-regulation of CCoAOMT decreases lignin amount whereas GSA accumulates to 10% of soluble phenolics indicates that endogenously produced sinapic acid is not a major precursor in syringyl lignin biosynthesis. Our in vivo results support the recently obtained in vitro enzymatic data that suggest that the route hom caffeic acid to sinapic acid is not used for lignin biosynthesis.
Keywords
PHENYLPROPANOID METABOLISM, VANILLA-PLANIFOLIA ANDR, SUBSTRATE SPECIFICITIES, A 3-O-METHYLTRANSFERASE, SUSPENSION-CULTURES, TRANSGENIC POPLARS, CELL-CULTURES, MAIZE LIGNIN, ACID, EXPRESSION

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Chicago
Meyermans, Hugo, Kris Morreel, Catherine Lapierre, Brigitte Pollet, André De Bruyn, Roger Busson, Piet Herdewijn, et al. 2000. “Modifications in Lignin and Accumulation of Phenolic Glucosides in Poplar Xylem Upon Down-regulation of Caffeoyl-coenzyme A O-methyltransferase, an Enzyme Involved in Lignin Biosynthesis.” Journal of Biological Chemistry 275 (47): 36899–36909.
APA
Meyermans, H., Morreel, K., Lapierre, C., Pollet, B., De Bruyn, A., Busson, R., Herdewijn, P., et al. (2000). Modifications in lignin and accumulation of phenolic glucosides in poplar xylem upon down-regulation of caffeoyl-coenzyme A O-methyltransferase, an enzyme involved in lignin biosynthesis. JOURNAL OF BIOLOGICAL CHEMISTRY, 275(47), 36899–36909.
Vancouver
1.
Meyermans H, Morreel K, Lapierre C, Pollet B, De Bruyn A, Busson R, et al. Modifications in lignin and accumulation of phenolic glucosides in poplar xylem upon down-regulation of caffeoyl-coenzyme A O-methyltransferase, an enzyme involved in lignin biosynthesis. JOURNAL OF BIOLOGICAL CHEMISTRY. 2000;275(47):36899–909.
MLA
Meyermans, Hugo, Kris Morreel, Catherine Lapierre, et al. “Modifications in Lignin and Accumulation of Phenolic Glucosides in Poplar Xylem Upon Down-regulation of Caffeoyl-coenzyme A O-methyltransferase, an Enzyme Involved in Lignin Biosynthesis.” JOURNAL OF BIOLOGICAL CHEMISTRY 275.47 (2000): 36899–36909. Print.
@article{125268,
  abstract     = {Caffeoyl-coenzyme A O-methyltransferase (CCoAOMT) methylates, in vitro, caffeoyl-CoA and 5-hydroxyferuloyl-CoA, two possible precursors in monolignol biosynthesis in vivo. To clarify the in vivo role of CCoAOMT in lignin biosynthesis, transgenic poplars with 10% residual CCoAOMT protein levels in the stem xylem were generated. Upon analysis of the xylem, the affected transgenic lines had a 12% reduced Klason lignin content, an 11% increased syringyl/guaiacyl ratio in the noncondensed lignin fraction, and an increase in lignin-attached p-hydroxybenzoate but otherwise a lignin composition similar to that of wild type. Stem xylem of the CCoAOMT-down-regulated lines had a pink-red coloration, which coincided with an enhanced fluorescence of mature vessel cell walls. The reduced production of CCoAOMT caused an accumulation of O-3-beta -D-glucopyranosyl-caffeic acid, O-4-beta -D-glucopyranosyl-vanillic acid, and O-4-beta -D-glucopyranosyl-sinapic acid (GSA), as authenticated by H-1 NMR Feeding experiments showed that O-3-beta -D-glucopyranosyl-caffeic acid and GSA are storage or detoxification products of caffeic and sinapic acid, respectively. The observation that down-regulation of CCoAOMT decreases lignin amount whereas GSA accumulates to 10% of soluble phenolics indicates that endogenously produced sinapic acid is not a major precursor in syringyl lignin biosynthesis. Our in vivo results support the recently obtained in vitro enzymatic data that suggest that the route hom caffeic acid to sinapic acid is not used for lignin biosynthesis.},
  author       = {Meyermans, Hugo and Morreel, Kris and Lapierre, Catherine and Pollet, Brigitte and De Bruyn, André and Busson, Roger and Herdewijn, Piet and Devreese, Bart and Van Beeumen, Jozef and Marita, Jane M and Ralph, John and Chen, Cuiying and Burggraeve, Bart and Van Montagu, Marc and Messens, Eric and Boerjan, Wout},
  issn         = {0021-9258},
  journal      = {JOURNAL OF BIOLOGICAL CHEMISTRY},
  keywords     = {PHENYLPROPANOID METABOLISM,VANILLA-PLANIFOLIA ANDR,SUBSTRATE SPECIFICITIES,A 3-O-METHYLTRANSFERASE,SUSPENSION-CULTURES,TRANSGENIC POPLARS,CELL-CULTURES,MAIZE LIGNIN,ACID,EXPRESSION},
  language     = {eng},
  number       = {47},
  pages        = {36899--36909},
  title        = {Modifications in lignin and accumulation of phenolic glucosides in poplar xylem upon down-regulation of caffeoyl-coenzyme A O-methyltransferase, an enzyme involved in lignin biosynthesis},
  url          = {http://dx.doi.org/10.1074/jbc.M006915200},
  volume       = {275},
  year         = {2000},
}

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