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Analysis of expressed sequence tags derived from a compatible Mycosphaerella fijiensis-banana interaction

Orelvis Portal Villafaña UGent, Yovanny Izquierdo, David De Vleesschauwer, Aminael Sánchez-Rodriguez, Milady Mendoza-Rodríguez, Mayra Acosta-Suárez, Bárbara Ocaña, Elio Jiménez and Monica Höfte UGent (2011) PLANT CELL REPORTS. 30(5). p.913-928
abstract
Mycosphaerella fijiensis, a hemibiotrophic fungus, is the causal agent of black leaf streak disease, the most serious foliar disease of bananas and plantains. To analyze the compatible interaction of M. fijiensis with Musa spp., a suppression subtractive hybridization (SSH) cDNA library was constructed to identify transcripts induced at late stages of infection in the host and the pathogen. In addition, a full-length cDNA library was created from the same mRNA starting material as the SSH library. The SSH procedure was effective in identifying specific genes predicted to be involved in plant-fungal interactions and new information was obtained mainly about genes and pathways activated in the plant. Several plant genes predicted to be involved in the synthesis of phenylpropanoids and detoxification compounds were identified, as well as pathogenesis-related proteins that could be involved in the plant response against M. fijiensis infection. At late stages of infection, jasmonic acid and ethylene signaling transduction pathways appear to be active, which corresponds with the necrotrophic life style of M. fijiensis. Quantitative PCR experiments revealed that antifungal genes encoding PR proteins and GDSL-like lipase are only transiently induced 30 days post inoculation (dpi), indicating that the fungus is probably actively repressing plant defense. The only fungal gene found was induced 37 dpi and encodes UDP-glucose pyrophosphorylase, an enzyme involved in the biosynthesis of trehalose. Trehalose biosynthesis was probably induced in response to prior activation of plant antifungal genes and may act as an osmoprotectant against membrane damage.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
BLACK LEAF STREAK, DEFENSE GENE-EXPRESSION, Plantain, Mycosphaerella fijiensis, Musa spp., Expressed sequence tags, cDNA libraries, Black sigatoka, Banana, LIPID-TRANSFER PROTEINS, FUNGUS MAGNAPORTHE-GRISEA, JASMONIC ACID, PLANT DEFENSE, MUSA SPP., ANTIFUNGAL ACTIVITY, HIGH-QUALITY, GLUTATHIONE HOMEOSTASIS
journal title
PLANT CELL REPORTS
Plant Cell Reports
volume
30
issue
5
pages
913 - 928
Web of Science type
Article
Web of Science id
000289481800020
JCR category
PLANT SCIENCES
JCR impact factor
2.274 (2011)
JCR rank
55/189 (2011)
JCR quartile
2 (2011)
ISSN
0721-7714
DOI
10.1007/s00299-011-1008-z
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1251461
handle
http://hdl.handle.net/1854/LU-1251461
date created
2011-06-01 15:13:08
date last changed
2016-12-19 15:42:48
@article{1251461,
  abstract     = {Mycosphaerella fijiensis, a hemibiotrophic fungus, is the causal agent of black leaf streak disease, the most serious foliar disease of bananas and plantains. To analyze the compatible interaction of M. fijiensis with Musa spp., a suppression subtractive hybridization (SSH) cDNA library was constructed to identify transcripts induced at late stages of infection in the host and the pathogen. In addition, a full-length cDNA library was created from the same mRNA starting material as the SSH library. The SSH procedure was effective in identifying specific genes predicted to be involved in plant-fungal interactions and new information was obtained mainly about genes and pathways activated in the plant. Several plant genes predicted to be involved in the synthesis of phenylpropanoids and detoxification compounds were identified, as well as pathogenesis-related proteins that could be involved in the plant response against M. fijiensis infection. At late stages of infection, jasmonic acid and ethylene signaling transduction pathways appear to be active, which corresponds with the necrotrophic life style of M. fijiensis. Quantitative PCR experiments revealed that antifungal genes encoding PR proteins and GDSL-like lipase are only transiently induced 30 days post inoculation (dpi), indicating that the fungus is probably actively repressing plant defense. The only fungal gene found was induced 37 dpi and encodes UDP-glucose pyrophosphorylase, an enzyme involved in the biosynthesis of trehalose. Trehalose biosynthesis was probably induced in response to prior activation of plant antifungal genes and may act as an osmoprotectant against membrane damage.},
  author       = {Portal Villafa{\~n}a, Orelvis and Izquierdo, Yovanny and De Vleesschauwer, David and S{\'a}nchez-Rodriguez, Aminael and Mendoza-Rodr{\'i}guez, Milady and Acosta-Su{\'a}rez, Mayra and Oca{\~n}a, B{\'a}rbara and Jim{\'e}nez, Elio and H{\"o}fte, Monica},
  issn         = {0721-7714},
  journal      = {PLANT CELL REPORTS},
  keyword      = {BLACK LEAF STREAK,DEFENSE GENE-EXPRESSION,Plantain,Mycosphaerella fijiensis,Musa spp.,Expressed sequence tags,cDNA libraries,Black sigatoka,Banana,LIPID-TRANSFER PROTEINS,FUNGUS MAGNAPORTHE-GRISEA,JASMONIC ACID,PLANT DEFENSE,MUSA SPP.,ANTIFUNGAL ACTIVITY,HIGH-QUALITY,GLUTATHIONE HOMEOSTASIS},
  language     = {eng},
  number       = {5},
  pages        = {913--928},
  title        = {Analysis of expressed sequence tags derived from a compatible Mycosphaerella fijiensis-banana interaction},
  url          = {http://dx.doi.org/10.1007/s00299-011-1008-z},
  volume       = {30},
  year         = {2011},
}

Chicago
Portal Villafaña, Orelvis, Yovanny Izquierdo, David De Vleesschauwer, Aminael Sánchez-Rodriguez, Milady Mendoza-Rodríguez, Mayra Acosta-Suárez, Bárbara Ocaña, Elio Jiménez, and Monica Höfte. 2011. “Analysis of Expressed Sequence Tags Derived from a Compatible Mycosphaerella Fijiensis-banana Interaction.” Plant Cell Reports 30 (5): 913–928.
APA
Portal Villafaña, O., Izquierdo, Y., De Vleesschauwer, D., Sánchez-Rodriguez, A., Mendoza-Rodríguez, M., Acosta-Suárez, M., Ocaña, B., et al. (2011). Analysis of expressed sequence tags derived from a compatible Mycosphaerella fijiensis-banana interaction. PLANT CELL REPORTS, 30(5), 913–928.
Vancouver
1.
Portal Villafaña O, Izquierdo Y, De Vleesschauwer D, Sánchez-Rodriguez A, Mendoza-Rodríguez M, Acosta-Suárez M, et al. Analysis of expressed sequence tags derived from a compatible Mycosphaerella fijiensis-banana interaction. PLANT CELL REPORTS. 2011;30(5):913–28.
MLA
Portal Villafaña, Orelvis, Yovanny Izquierdo, David De Vleesschauwer, et al. “Analysis of Expressed Sequence Tags Derived from a Compatible Mycosphaerella Fijiensis-banana Interaction.” PLANT CELL REPORTS 30.5 (2011): 913–928. Print.