Advanced search
1 file | 465.87 KB

The ROOT MERISTEMLESS1/CADMIUM SENSITIVE2 gene defines a glutathione-dependent pathway involved in initiation and maintenance of cell division during postembryonic root development

(2000) PLANT CELL. 12(1). p.97-109
Author
Organization
Abstract
Activation of cell division in the root apical meristem after germination is essential for postembryonic root development. Arabidopsis plants homozygous for a mutation in the ROOT MERISTEMLESS1 (RML1) gene are unable to establish an active postembryonic meristem in the root apex, This mutation abolishes cell division in the root but not in the shoot. We report the molecular cloning of the RML1 gene, which encodes the first enzyme of glutathione (GSH) biosynthesis, gamma-glutamylcysteine synthetase, and which is allelic to CADMIUM SENSITIVE2. The phenotype of the rml1 mutant, which was also evident in the roots of wild-type Arabidopsis and tobacco treated with an inhibitor of GSH biosynthesis, could be relieved by applying GSH to rml1 seedlings. By using a synchronized tobacco cell suspension culture, we showed that the G(1)-to-S phase transition requires an adequate level of GSH, These observations suggest the existence of a GSH-dependent developmental pathway essential for initiation and maintenance of cell division during postembryonic root development.
Keywords
ACTIVATION, MODULATION, DEFICIENT, EXPRESSION, ORGANIZATION, MERISTEM, BINDING-ACTIVITY, PLANT DEVELOPMENT, GAMMA-GLUTAMYLCYSTEINE SYNTHETASE, ARABIDOPSIS-THALIANA

Downloads

    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 465.87 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Vernoux, Teva, Robert C Wilson, Kevin A Seeley, Jean-Philippe Reichheld, Sandra Muroy, Spencer Brown, Spencer C Maughan, et al. 2000. “The ROOT MERISTEMLESS1/CADMIUM SENSITIVE2 Gene Defines a Glutathione-dependent Pathway Involved in Initiation and Maintenance of Cell Division During Postembryonic Root Development.” Plant Cell 12 (1): 97–109.
APA
Vernoux, T., Wilson, R. C., Seeley, K. A., Reichheld, J.-P., Muroy, S., Brown, S., Maughan, S. C., et al. (2000). The ROOT MERISTEMLESS1/CADMIUM SENSITIVE2 gene defines a glutathione-dependent pathway involved in initiation and maintenance of cell division during postembryonic root development. PLANT CELL, 12(1), 97–109.
Vancouver
1.
Vernoux T, Wilson RC, Seeley KA, Reichheld J-P, Muroy S, Brown S, et al. The ROOT MERISTEMLESS1/CADMIUM SENSITIVE2 gene defines a glutathione-dependent pathway involved in initiation and maintenance of cell division during postembryonic root development. PLANT CELL. 2000;12(1):97–109.
MLA
Vernoux, Teva, Robert C Wilson, Kevin A Seeley, et al. “The ROOT MERISTEMLESS1/CADMIUM SENSITIVE2 Gene Defines a Glutathione-dependent Pathway Involved in Initiation and Maintenance of Cell Division During Postembryonic Root Development.” PLANT CELL 12.1 (2000): 97–109. Print.
@article{125044,
  abstract     = {Activation of cell division in the root apical meristem after germination is essential for postembryonic root development. Arabidopsis plants homozygous for a mutation in the ROOT MERISTEMLESS1 (RML1) gene are unable to establish an active postembryonic meristem in the root apex, This mutation abolishes cell division in the root but not in the shoot. We report the molecular cloning of the RML1 gene, which encodes the first enzyme of glutathione (GSH) biosynthesis, gamma-glutamylcysteine synthetase, and which is allelic to CADMIUM SENSITIVE2. The phenotype of the rml1 mutant, which was also evident in the roots of wild-type Arabidopsis and tobacco treated with an inhibitor of GSH biosynthesis, could be relieved by applying GSH to rml1 seedlings. By using a synchronized tobacco cell suspension culture, we showed that the G(1)-to-S phase transition requires an adequate level of GSH, These observations suggest the existence of a GSH-dependent developmental pathway essential for initiation and maintenance of cell division during postembryonic root development.},
  author       = {Vernoux, Teva and Wilson, Robert C and Seeley, Kevin A and Reichheld, Jean-Philippe and Muroy, Sandra and Brown, Spencer and Maughan, Spencer C and Cobbett, Christopher S and Van Montagu, Marc and Inz{\'e}, Dirk and May, Mike and Sung, Zinmay R},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {ACTIVATION,MODULATION,DEFICIENT,EXPRESSION,ORGANIZATION,MERISTEM,BINDING-ACTIVITY,PLANT DEVELOPMENT,GAMMA-GLUTAMYLCYSTEINE SYNTHETASE,ARABIDOPSIS-THALIANA},
  language     = {eng},
  number       = {1},
  pages        = {97--109},
  title        = {The ROOT MERISTEMLESS1/CADMIUM SENSITIVE2 gene defines a glutathione-dependent pathway involved in initiation and maintenance of cell division during postembryonic root development},
  url          = {http://dx.doi.org/10.1105/tpc.12.1.97},
  volume       = {12},
  year         = {2000},
}

Altmetric
View in Altmetric
Web of Science
Times cited: