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Cell division events are essential for embryo patterning and morphogenesis: studies on dominant-negative cdc2aAt mutants of Arabidopsis

(2000) PLANT JOURNAL. 23(1). p.123-130
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Abstract
During plant development, cell division events are coordinately regulated, leading to specific growth patterns. Experimental evidence indicates that the morphogenetic controls that act at the vegetative plant growth stage are flexible and tolerate distortions in patterns and frequencies of cell division. To address questions concerning the relationship between cell division and embryo formation, a novel experimental approach was used. The frequencies of cell division were reduced exclusively during embryo development of Arabidopsis by the expression of a dominant cdc2a mutant. The five independent transgenic lines with the highest levels of the mutant cdc2a affected embryo formation. In the C13 line, seeds failed to germinate. The C1, C5 and C12 lines displayed a range of distortions on the apical-basal embryo pattern. In the C3 line, the shoot apical meristem of the seedlings produced leaves defective in growth and with an incorrect phyllotactic pattern. The results demonstrate that rates of cell division do not dictate cellular differentiation of embryos. Nevertheless, whereas cell divisions are uncoupled from vegetative development, they are instrumental in elaborating embryo structures and modulating embryo and seedling morphogenesis.
Keywords
cell division, apical-basal pattern, dominant negative cdc2a mutant, embryogenesis, morphogenesis, CYCLIN-DEPENDENT KINASES, 2S ALBUMIN GENES, PLANT DEVELOPMENT, EXPRESSION, EMBRYOGENESIS, MUTATIONS, MERISTEM, PROTEIN, FATE, TRANSFORMATION

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Chicago
Hemerly, Adriana S, Paulo CG Ferreira, Marc Van Montagu, Gilbert Engler, and Dirk Inzé. 2000. “Cell Division Events Are Essential for Embryo Patterning and Morphogenesis: Studies on Dominant-negative cdc2aAt Mutants of Arabidopsis.” Plant Journal 23 (1): 123–130.
APA
Hemerly, A. S., Ferreira, P. C., Van Montagu, M., Engler, G., & Inzé, D. (2000). Cell division events are essential for embryo patterning and morphogenesis: studies on dominant-negative cdc2aAt mutants of Arabidopsis. PLANT JOURNAL, 23(1), 123–130.
Vancouver
1.
Hemerly AS, Ferreira PC, Van Montagu M, Engler G, Inzé D. Cell division events are essential for embryo patterning and morphogenesis: studies on dominant-negative cdc2aAt mutants of Arabidopsis. PLANT JOURNAL. 2000;23(1):123–30.
MLA
Hemerly, Adriana S, Paulo CG Ferreira, Marc Van Montagu, et al. “Cell Division Events Are Essential for Embryo Patterning and Morphogenesis: Studies on Dominant-negative cdc2aAt Mutants of Arabidopsis.” PLANT JOURNAL 23.1 (2000): 123–130. Print.
@article{124950,
  abstract     = {During plant development, cell division events are coordinately regulated, leading to specific growth patterns. Experimental evidence indicates that the morphogenetic controls that act at the vegetative plant growth stage are flexible and tolerate distortions in patterns and frequencies of cell division. To address questions concerning the relationship between cell division and embryo formation, a novel experimental approach was used. The frequencies of cell division were reduced exclusively during embryo development of Arabidopsis by the expression of a dominant cdc2a mutant. The five independent transgenic lines with the highest levels of the mutant cdc2a affected embryo formation. In the C13 line, seeds failed to germinate. The C1, C5 and C12 lines displayed a range of distortions on the apical-basal embryo pattern. In the C3 line, the shoot apical meristem of the seedlings produced leaves defective in growth and with an incorrect phyllotactic pattern. The results demonstrate that rates of cell division do not dictate cellular differentiation of embryos. Nevertheless, whereas cell divisions are uncoupled from vegetative development, they are instrumental in elaborating embryo structures and modulating embryo and seedling morphogenesis.},
  author       = {Hemerly, Adriana S and Ferreira, Paulo CG and Van Montagu, Marc and Engler, Gilbert and Inz{\'e}, Dirk},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  language     = {eng},
  number       = {1},
  pages        = {123--130},
  title        = {Cell division events are essential for embryo patterning and morphogenesis: studies on dominant-negative cdc2aAt mutants of Arabidopsis},
  url          = {http://dx.doi.org/10.1046/j.1365-313x.2000.00800.x},
  volume       = {23},
  year         = {2000},
}

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