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Three major somatic embryogenesis related proteins in Cichorium identified as PR proteins

Stéphane Helleboid, Theo Hendriks, Guy Bauw UGent, Dirk Inzé UGent, Jacques Vasseur and Jean-Louis Hilbert (2000) JOURNAL OF EXPERIMENTAL BOTANY. 51(348). p.1189-1200
abstract
In Cichorium hybrid clone '474' (C, intybus L., var. sativum x C, endivia L., var. latifolia), the direct somatic embryogenesis process in leaf tissues is accompanied by an overall increase in the amount of proteins secreted into the culture medium, Amongst these, three major protein bands of 38 kDa, 32 kDa and 25 kDa were found in the conditioned media. These extracellular protein bands accumulated in the medium of the embryogenic Cichorium hybrid up to 8-fold compared with those in the medium of a nonembryogenic variety, 32 and 25 kDa proteins were purified from the medium and their identities were determined as already described for 38 kDa beta-1,3-glucanases, To investigate their possible function in somatic embryogenesis, peptide sequences, serological relationships or biochemical properties revealed that there were at least two acidic chitinases of 32 kDa and one glycosylated osmotin-like protein of 25 kDa in the embryogenic culture medium. Comparing the amounts of the 38 kDa glucanases, the 32 kDa chitinases, and the 25 kDa osmotin-like protein present in the conditioned media of the embryogenic '474' hybrid and of a non-embryogenic variety, a 2-8-fold higher accumulation of these proteins was observed in the embryogenic hybrid culture medium. This may suggest that part of the accumulation of these three pathogenesis-related (PR) proteins could be correlated with the somatic embryogenesis process, Their possible involvement in this developmental process is discussed.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
leaves, Cichorium, somatic embryogenesis, extracellular proteins, beta-1, 3-glucanases, chitinases, osmotin-like proteins, microsequencing, immunoblots, PATHOGENESIS-RELATED PROTEINS, THAUMATIN-LIKE PROTEIN, TOBACCO MOSAIC-VIRUS, MESSENGER-RNA ACCUMULATION, BETA-1, 3-GLUCANASE GENES, PHYTOPHTHORA-INFESTANS, DEVELOPMENTAL SIGNALS, ANTIFUNGAL PROTEINS, SUSPENSION-CULTURES, INHIBITORY ACTIVITY
journal title
JOURNAL OF EXPERIMENTAL BOTANY
J. Exp. Bot.
volume
51
issue
348
pages
1189 - 1200
Web of Science type
Article
Web of Science id
000088519300003
ISSN
0022-0957
DOI
10.1093/jexbot/51.348.1189
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
124944
handle
http://hdl.handle.net/1854/LU-124944
date created
2004-01-14 13:36:00
date last changed
2016-12-19 15:37:49
@article{124944,
  abstract     = {In Cichorium hybrid clone '474' (C, intybus L., var. sativum x C, endivia L., var. latifolia), the direct somatic embryogenesis process in leaf tissues is accompanied by an overall increase in the amount of proteins secreted into the culture medium, Amongst these, three major protein bands of 38 kDa, 32 kDa and 25 kDa were found in the conditioned media. These extracellular protein bands accumulated in the medium of the embryogenic Cichorium hybrid up to 8-fold compared with those in the medium of a nonembryogenic variety, 32 and 25 kDa proteins were purified from the medium and their identities were determined as already described for 38 kDa beta-1,3-glucanases, To investigate their possible function in somatic embryogenesis, peptide sequences, serological relationships or biochemical properties revealed that there were at least two acidic chitinases of 32 kDa and one glycosylated osmotin-like protein of 25 kDa in the embryogenic culture medium. Comparing the amounts of the 38 kDa glucanases, the 32 kDa chitinases, and the 25 kDa osmotin-like protein present in the conditioned media of the embryogenic '474' hybrid and of a non-embryogenic variety, a 2-8-fold higher accumulation of these proteins was observed in the embryogenic hybrid culture medium. This may suggest that part of the accumulation of these three pathogenesis-related (PR) proteins could be correlated with the somatic embryogenesis process, Their possible involvement in this developmental process is discussed.},
  author       = {Helleboid, St{\'e}phane and Hendriks, Theo and Bauw, Guy and Inz{\'e}, Dirk and Vasseur, Jacques and Hilbert, Jean-Louis},
  issn         = {0022-0957},
  journal      = {JOURNAL OF EXPERIMENTAL BOTANY},
  keyword      = {leaves,Cichorium,somatic embryogenesis,extracellular proteins,beta-1,3-glucanases,chitinases,osmotin-like proteins,microsequencing,immunoblots,PATHOGENESIS-RELATED PROTEINS,THAUMATIN-LIKE PROTEIN,TOBACCO MOSAIC-VIRUS,MESSENGER-RNA ACCUMULATION,BETA-1,3-GLUCANASE GENES,PHYTOPHTHORA-INFESTANS,DEVELOPMENTAL SIGNALS,ANTIFUNGAL PROTEINS,SUSPENSION-CULTURES,INHIBITORY ACTIVITY},
  language     = {eng},
  number       = {348},
  pages        = {1189--1200},
  title        = {Three major somatic embryogenesis related proteins in Cichorium identified as PR proteins},
  url          = {http://dx.doi.org/10.1093/jexbot/51.348.1189},
  volume       = {51},
  year         = {2000},
}

Chicago
Helleboid, Stéphane, Theo Hendriks, Guy Bauw, Dirk Inzé, Jacques Vasseur, and Jean-Louis Hilbert. 2000. “Three Major Somatic Embryogenesis Related Proteins in Cichorium Identified as PR Proteins.” Journal of Experimental Botany 51 (348): 1189–1200.
APA
Helleboid, S., Hendriks, T., Bauw, G., Inzé, D., Vasseur, J., & Hilbert, J.-L. (2000). Three major somatic embryogenesis related proteins in Cichorium identified as PR proteins. JOURNAL OF EXPERIMENTAL BOTANY, 51(348), 1189–1200.
Vancouver
1.
Helleboid S, Hendriks T, Bauw G, Inzé D, Vasseur J, Hilbert J-L. Three major somatic embryogenesis related proteins in Cichorium identified as PR proteins. JOURNAL OF EXPERIMENTAL BOTANY. 2000;51(348):1189–200.
MLA
Helleboid, Stéphane, Theo Hendriks, Guy Bauw, et al. “Three Major Somatic Embryogenesis Related Proteins in Cichorium Identified as PR Proteins.” JOURNAL OF EXPERIMENTAL BOTANY 51.348 (2000): 1189–1200. Print.