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Analysis of bruchid resistance in the wild common bean accession G02771: no evidence for insecticidal activity of arcelin 5

(2000) JOURNAL OF EXPERIMENTAL BOTANY. 51(348). p.1229-1236
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Abstract
Arcelins are abundant seed storage proteins thought to be implicated in the resistance of wild Phaseolus vulgaris (L.) genotypes against Zabrotes subfasciatus (Boheman), an important storage insect pest of common bean. Here, the insecticidal activity of the arcelin-5 variant that is present in the highly resistant P. vulgaris accession G02771 was investigated. No correlation could be established between the presence of arcelin 5 and the insecticidal effects observed in G02771 seeds. Insect feeding assays with artificial seeds into which purified arcelin-5 protein was incorporated and with transgenic P, acutifolius (A, Gray) seeds in which the arcelin-5 genes were expressed, showed that the presence of arcelin-5 proteins, even at elevated levels, was not sufficient to achieve adequate resistance against Z. subfasciatus, The same might apply to other arcelin variants. Nevertheless, as resistance is clearly closely linked to the presence of the arcelin-1 or arcelin-5 locus, arcelins remain useful markers in breeding programmes aimed at introgressing high levels of resistance to Z. subfasciatus in P. vulgaris cultivars.
Keywords
POSTHARVEST INFESTATION, SEED PROTEIN, COWPEA WEEVIL, L CULTIVAR, INHERITANCE, GROWTH, arcelin, bruchid resistance, Mexican bean weevil, Phaseolus, Zabrotes subfasciatus, WEEVIL COLEOPTERA, SUBFASCIATUS BOHEMAN COLEOPTERA, PHASEOLUS-VULGARIS L, ALPHA-AMYLASE INHIBITOR

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Citation

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MLA
Goossens, Alain, Constanza Quintero, Willy Dillen, et al. “Analysis of Bruchid Resistance in the Wild Common Bean Accession G02771: No Evidence for Insecticidal Activity of Arcelin 5.” JOURNAL OF EXPERIMENTAL BOTANY 51.348 (2000): 1229–1236. Print.
APA
Goossens, Alain, Quintero, C., Dillen, W., De Rycke, R., Valor, J. F., De Clercq, J., Van Montagu, M., et al. (2000). Analysis of bruchid resistance in the wild common bean accession G02771: no evidence for insecticidal activity of arcelin 5. JOURNAL OF EXPERIMENTAL BOTANY, 51(348), 1229–1236.
Chicago author-date
Goossens, Alain, Constanza Quintero, Willy Dillen, Riet De Rycke, Jose Flower Valor, Janniek De Clercq, Marc Van Montagu, Cesar Cardona, and Geert Angenon. 2000. “Analysis of Bruchid Resistance in the Wild Common Bean Accession G02771: No Evidence for Insecticidal Activity of Arcelin 5.” Journal of Experimental Botany 51 (348): 1229–1236.
Chicago author-date (all authors)
Goossens, Alain, Constanza Quintero, Willy Dillen, Riet De Rycke, Jose Flower Valor, Janniek De Clercq, Marc Van Montagu, Cesar Cardona, and Geert Angenon. 2000. “Analysis of Bruchid Resistance in the Wild Common Bean Accession G02771: No Evidence for Insecticidal Activity of Arcelin 5.” Journal of Experimental Botany 51 (348): 1229–1236.
Vancouver
1.
Goossens A, Quintero C, Dillen W, De Rycke R, Valor JF, De Clercq J, et al. Analysis of bruchid resistance in the wild common bean accession G02771: no evidence for insecticidal activity of arcelin 5. JOURNAL OF EXPERIMENTAL BOTANY. 2000;51(348):1229–36.
IEEE
[1]
A. Goossens et al., “Analysis of bruchid resistance in the wild common bean accession G02771: no evidence for insecticidal activity of arcelin 5,” JOURNAL OF EXPERIMENTAL BOTANY, vol. 51, no. 348, pp. 1229–1236, 2000.
@article{124927,
  abstract     = {Arcelins are abundant seed storage proteins thought to be implicated in the resistance of wild Phaseolus vulgaris (L.) genotypes against Zabrotes subfasciatus (Boheman), an important storage insect pest of common bean. Here, the insecticidal activity of the arcelin-5 variant that is present in the highly resistant P. vulgaris accession G02771 was investigated. No correlation could be established between the presence of arcelin 5 and the insecticidal effects observed in G02771 seeds. Insect feeding assays with artificial seeds into which purified arcelin-5 protein was incorporated and with transgenic P, acutifolius (A, Gray) seeds in which the arcelin-5 genes were expressed, showed that the presence of arcelin-5 proteins, even at elevated levels, was not sufficient to achieve adequate resistance against Z. subfasciatus, The same might apply to other arcelin variants. Nevertheless, as resistance is clearly closely linked to the presence of the arcelin-1 or arcelin-5 locus, arcelins remain useful markers in breeding programmes aimed at introgressing high levels of resistance to Z. subfasciatus in P. vulgaris cultivars.},
  author       = {Goossens, Alain and Quintero, Constanza and Dillen, Willy and De Rycke, Riet and Valor, Jose Flower and De Clercq, Janniek and Van Montagu, Marc and Cardona, Cesar and Angenon, Geert},
  issn         = {0022-0957},
  journal      = {JOURNAL OF EXPERIMENTAL BOTANY},
  keywords     = {POSTHARVEST INFESTATION,SEED PROTEIN,COWPEA WEEVIL,L CULTIVAR,INHERITANCE,GROWTH,arcelin,bruchid resistance,Mexican bean weevil,Phaseolus,Zabrotes subfasciatus,WEEVIL COLEOPTERA,SUBFASCIATUS BOHEMAN COLEOPTERA,PHASEOLUS-VULGARIS L,ALPHA-AMYLASE INHIBITOR},
  language     = {eng},
  number       = {348},
  pages        = {1229--1236},
  title        = {Analysis of bruchid resistance in the wild common bean accession G02771: no evidence for insecticidal activity of arcelin 5},
  url          = {http://dx.doi.org/10.1093/jexbot/51.348.1229},
  volume       = {51},
  year         = {2000},
}

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