Advanced search
1 file | 531.43 KB

Applications of flow cytometry in plant pathology for genome size determination, detection and physiological status

(2011) MOLECULAR PLANT PATHOLOGY. 12(8). p.815-828
Author
Organization
Project
Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Flow cytometers are probably the most multipurpose laboratory devices available. They can analyse a vast and very diverse range of cell parameters. This technique has left its mark on cancer, human immunodeficiency virus and immunology research, and is indispensable in routine clinical diagnostics. Flow cytometry (FCM) is also a well-known tool for the detection and physiological status assessment of microorganisms in drinking water, marine environments, food and fermentation processes. However, flow cytometers are seldom used in plant pathology, despite FCM’s major advantages as both a detection method and a research tool. Potential uses of FCM include the characterization of genome sizes of fungal and oomycete populations, multiplexed pathogen detection and the monitoring of the viability, culturability and gene expression of plant pathogens, and many others.This review provides an overview of the history, advantages and disadvantages of FCM, and focuses on the current applications and future possibilities of FCM in plant pathology.
Keywords
NUCLEAR-DNA CONTENT, MICHIGANENSIS SUBSP MICHIGANENSIS, BUT-NONCULTURABLE STATE, LINKED IMMUNOSORBENT ASSAY, POLYMERASE CHAIN-REACTION, FEULGEN IMAGE-ANALYSIS, CUCUMBER MOSAIC-VIRUS, PATHOGENIC BACTERIA, PROPIDIUM IODIDE, ENVIRONMENTAL MICROBIOLOGY

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 531.43 KB

Citation

Please use this url to cite or link to this publication:

Chicago
D’hondt, Liesbet, Monica Höfte, Erik Van Bockstaele, and Leen Leus. 2011. “Applications of Flow Cytometry in Plant Pathology for Genome Size Determination, Detection and Physiological Status.” Molecular Plant Pathology 12 (8): 815–828.
APA
D’hondt, L., Höfte, M., Van Bockstaele, E., & Leus, L. (2011). Applications of flow cytometry in plant pathology for genome size determination, detection and physiological status. MOLECULAR PLANT PATHOLOGY, 12(8), 815–828.
Vancouver
1.
D’hondt L, Höfte M, Van Bockstaele E, Leus L. Applications of flow cytometry in plant pathology for genome size determination, detection and physiological status. MOLECULAR PLANT PATHOLOGY. 2011;12(8):815–28.
MLA
D’hondt, Liesbet, Monica Höfte, Erik Van Bockstaele, et al. “Applications of Flow Cytometry in Plant Pathology for Genome Size Determination, Detection and Physiological Status.” MOLECULAR PLANT PATHOLOGY 12.8 (2011): 815–828. Print.
@article{1249062,
  abstract     = {Flow cytometers are probably the most multipurpose laboratory devices available. They can analyse a vast and very diverse range of cell parameters. This technique has left its mark on cancer, human immunodeficiency virus and immunology research, and is indispensable in routine clinical diagnostics. Flow cytometry (FCM) is also a well-known tool for the detection and physiological status assessment of microorganisms in drinking water, marine environments, food and fermentation processes. However, flow cytometers are seldom used in plant pathology, despite FCM{\textquoteright}s major advantages as both a detection method and a research tool. Potential uses of FCM include the characterization of genome sizes of fungal and oomycete populations, multiplexed pathogen detection and the monitoring of the viability, culturability and gene expression of plant pathogens, and many others.This review provides an overview of the history, advantages and disadvantages of FCM, and focuses on the current applications and future possibilities of FCM in plant pathology.},
  author       = {D'hondt, Liesbet and H{\"o}fte, Monica and Van Bockstaele, Erik and Leus, Leen},
  issn         = {1464-6722},
  journal      = {MOLECULAR PLANT PATHOLOGY},
  keyword      = {NUCLEAR-DNA CONTENT,MICHIGANENSIS SUBSP MICHIGANENSIS,BUT-NONCULTURABLE STATE,LINKED IMMUNOSORBENT ASSAY,POLYMERASE CHAIN-REACTION,FEULGEN IMAGE-ANALYSIS,CUCUMBER MOSAIC-VIRUS,PATHOGENIC BACTERIA,PROPIDIUM IODIDE,ENVIRONMENTAL MICROBIOLOGY},
  language     = {eng},
  number       = {8},
  pages        = {815--828},
  title        = {Applications of flow cytometry in plant pathology for genome size determination, detection and physiological status},
  url          = {http://dx.doi.org/10.1111/j.1364-3703.2011.00711.x},
  volume       = {12},
  year         = {2011},
}

Altmetric
View in Altmetric
Web of Science
Times cited: