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Applications of flow cytometry in plant pathology for genome size determination, detection and physiological status

Liesbet D'hondt, Monica Höfte UGent, Erik Van Bockstaele and Leen Leus (2011) MOLECULAR PLANT PATHOLOGY. 12(8). p.815-828
abstract
Flow cytometers are probably the most multipurpose laboratory devices available. They can analyse a vast and very diverse range of cell parameters. This technique has left its mark on cancer, human immunodeficiency virus and immunology research, and is indispensable in routine clinical diagnostics. Flow cytometry (FCM) is also a well-known tool for the detection and physiological status assessment of microorganisms in drinking water, marine environments, food and fermentation processes. However, flow cytometers are seldom used in plant pathology, despite FCM’s major advantages as both a detection method and a research tool. Potential uses of FCM include the characterization of genome sizes of fungal and oomycete populations, multiplexed pathogen detection and the monitoring of the viability, culturability and gene expression of plant pathogens, and many others.This review provides an overview of the history, advantages and disadvantages of FCM, and focuses on the current applications and future possibilities of FCM in plant pathology.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
NUCLEAR-DNA CONTENT, MICHIGANENSIS SUBSP MICHIGANENSIS, BUT-NONCULTURABLE STATE, LINKED IMMUNOSORBENT ASSAY, POLYMERASE CHAIN-REACTION, FEULGEN IMAGE-ANALYSIS, CUCUMBER MOSAIC-VIRUS, PATHOGENIC BACTERIA, PROPIDIUM IODIDE, ENVIRONMENTAL MICROBIOLOGY
journal title
MOLECULAR PLANT PATHOLOGY
Mol. Plant Pathol.
volume
12
issue
8
pages
815 - 828
Web of Science type
Article
Web of Science id
000294562100009
JCR category
PLANT SCIENCES
JCR impact factor
3.899 (2011)
JCR rank
20/189 (2011)
JCR quartile
1 (2011)
ISSN
1464-6722
DOI
10.1111/j.1364-3703.2011.00711.x
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1249062
handle
http://hdl.handle.net/1854/LU-1249062
date created
2011-05-31 13:43:30
date last changed
2016-12-19 15:42:48
@article{1249062,
  abstract     = {Flow cytometers are probably the most multipurpose laboratory devices available. They can analyse a vast and very diverse range of cell parameters. This technique has left its mark on cancer, human immunodeficiency virus and immunology research, and is indispensable in routine clinical diagnostics. Flow cytometry (FCM) is also a well-known tool for the detection and physiological status assessment of microorganisms in drinking water, marine environments, food and fermentation processes. However, flow cytometers are seldom used in plant pathology, despite FCM{\textquoteright}s major advantages as both a detection method and a research tool. Potential uses of FCM include the characterization of genome sizes of fungal and oomycete populations, multiplexed pathogen detection and the monitoring of the viability, culturability and gene expression of plant pathogens, and many others.This review provides an overview of the history, advantages and disadvantages of FCM, and focuses on the current applications and future possibilities of FCM in plant pathology.},
  author       = {D'hondt, Liesbet and H{\"o}fte, Monica and Van Bockstaele, Erik and Leus, Leen},
  issn         = {1464-6722},
  journal      = {MOLECULAR PLANT PATHOLOGY},
  keyword      = {NUCLEAR-DNA CONTENT,MICHIGANENSIS SUBSP MICHIGANENSIS,BUT-NONCULTURABLE STATE,LINKED IMMUNOSORBENT ASSAY,POLYMERASE CHAIN-REACTION,FEULGEN IMAGE-ANALYSIS,CUCUMBER MOSAIC-VIRUS,PATHOGENIC BACTERIA,PROPIDIUM IODIDE,ENVIRONMENTAL MICROBIOLOGY},
  language     = {eng},
  number       = {8},
  pages        = {815--828},
  title        = {Applications of flow cytometry in plant pathology for genome size determination, detection and physiological status},
  url          = {http://dx.doi.org/10.1111/j.1364-3703.2011.00711.x},
  volume       = {12},
  year         = {2011},
}

Chicago
D’hondt, Liesbet, Monica Höfte, Erik Van Bockstaele, and Leen Leus. 2011. “Applications of Flow Cytometry in Plant Pathology for Genome Size Determination, Detection and Physiological Status.” Molecular Plant Pathology 12 (8): 815–828.
APA
D’hondt, L., Höfte, M., Van Bockstaele, E., & Leus, L. (2011). Applications of flow cytometry in plant pathology for genome size determination, detection and physiological status. MOLECULAR PLANT PATHOLOGY, 12(8), 815–828.
Vancouver
1.
D’hondt L, Höfte M, Van Bockstaele E, Leus L. Applications of flow cytometry in plant pathology for genome size determination, detection and physiological status. MOLECULAR PLANT PATHOLOGY. 2011;12(8):815–28.
MLA
D’hondt, Liesbet, Monica Höfte, Erik Van Bockstaele, et al. “Applications of Flow Cytometry in Plant Pathology for Genome Size Determination, Detection and Physiological Status.” MOLECULAR PLANT PATHOLOGY 12.8 (2011): 815–828. Print.