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Nod factor requirements for efficient stem and root nodulation of the tropical legume Sesbania rostrata

Wim D'Haeze UGent, Peter Mergaert UGent, Jean-Claude Promé and Marcella Holsters UGent (2000) JOURNAL OF BIOLOGICAL CHEMISTRY. 275(21). p.15676-15684
abstract
Azorhizobium caulinodans ORS571 synthesizes mainly pentameric Nod factors with a household fatty acid, an N-methyl, and a 6-O-carbamoyl group at the nonreducing-terminal residue and with a D-arabinosyl, an L-fucosyl group, or both at the reducing-terminal residue. Nodulation on Sesbania rostrata was carried out with a set of bacterial mutants that produce well characterized Nod factor populations. Purified Nod factors were tested for their capacity to induce root hair formation and for their stability in an in vitro degradation assay with extracts of uninfected adventitious rootlets. The glycosylations increased synergistically the nodulation efficiency and the capacity to induce root hairs, and they protected the Nod factor against degradation. The D-arabinosyl group was more important than the L-fucosyl group for nodulation efficiency. Replacement of the 6-O-L-fucosyl group by a 6-O-sulfate ester did not affect Nod factor stability, but reduced nodulation efficiency, indicating that the L-fucosyl group may play a role in recognition. The 6-O-carbamoyl group contributes to nodulation efficiency, biological activity, and protection, but could be replaced by a 6-O-acetyl group for root nodulation. The results demonstrate that none of the studied substitutions is strictly required for triggering normal nodule formation. However, the nodulation efficiency was greatly determined by the synergistic presence of substitutions. Within the range tested, fluctuations of Nod factor amounts had little impact on the symbiotic phenotype.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
LIPO-OLIGOSACCHARIDE SIGNALS, VITRO SULFOTRANSFERASE ACTIVITY, AZORHIZOBIUM-CAULINODANS, RHIZOBIUM-MELILOTI, HOST-RANGE, ESCHERICHIA-COLI, IN-VITRO, STRUCTURAL MODIFICATIONS, SUBSTRATE-SPECIFICITY, SINORHIZOBIUM-SAHELI
journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
J. Biol. Chem.
volume
275
issue
21
pages
15676 - 15684
Web of Science type
Article
Web of Science id
000087291400013
ISSN
0021-9258
DOI
10.1074/jbc.275.21.15676
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
124886
handle
http://hdl.handle.net/1854/LU-124886
date created
2004-01-14 13:36:00
date last changed
2013-10-03 12:52:47
@article{124886,
  abstract     = {Azorhizobium caulinodans ORS571 synthesizes mainly pentameric Nod factors with a household fatty acid, an N-methyl, and a 6-O-carbamoyl group at the nonreducing-terminal residue and with a D-arabinosyl, an L-fucosyl group, or both at the reducing-terminal residue. Nodulation on Sesbania rostrata was carried out with a set of bacterial mutants that produce well characterized Nod factor populations. Purified Nod factors were tested for their capacity to induce root hair formation and for their stability in an in vitro degradation assay with extracts of uninfected adventitious rootlets. The glycosylations increased synergistically the nodulation efficiency and the capacity to induce root hairs, and they protected the Nod factor against degradation. The D-arabinosyl group was more important than the L-fucosyl group for nodulation efficiency. Replacement of the 6-O-L-fucosyl group by a 6-O-sulfate ester did not affect Nod factor stability, but reduced nodulation efficiency, indicating that the L-fucosyl group may play a role in recognition. The 6-O-carbamoyl group contributes to nodulation efficiency, biological activity, and protection, but could be replaced by a 6-O-acetyl group for root nodulation. The results demonstrate that none of the studied substitutions is strictly required for triggering normal nodule formation. However, the nodulation efficiency was greatly determined by the synergistic presence of substitutions. Within the range tested, fluctuations of Nod factor amounts had little impact on the symbiotic phenotype.},
  author       = {D'Haeze, Wim and Mergaert, Peter and Prom{\'e}, Jean-Claude and Holsters, Marcella},
  issn         = {0021-9258},
  journal      = {JOURNAL OF BIOLOGICAL CHEMISTRY},
  keyword      = {LIPO-OLIGOSACCHARIDE SIGNALS,VITRO SULFOTRANSFERASE ACTIVITY,AZORHIZOBIUM-CAULINODANS,RHIZOBIUM-MELILOTI,HOST-RANGE,ESCHERICHIA-COLI,IN-VITRO,STRUCTURAL MODIFICATIONS,SUBSTRATE-SPECIFICITY,SINORHIZOBIUM-SAHELI},
  language     = {eng},
  number       = {21},
  pages        = {15676--15684},
  title        = {Nod factor requirements for efficient stem and root nodulation of the tropical legume Sesbania rostrata},
  url          = {http://dx.doi.org/10.1074/jbc.275.21.15676},
  volume       = {275},
  year         = {2000},
}

Chicago
D’Haeze, Wim, Peter Mergaert, Jean-Claude Promé, and Marcella Holsters. 2000. “Nod Factor Requirements for Efficient Stem and Root Nodulation of the Tropical Legume Sesbania Rostrata.” Journal of Biological Chemistry 275 (21): 15676–15684.
APA
D’Haeze, W., Mergaert, P., Promé, J.-C., & Holsters, M. (2000). Nod factor requirements for efficient stem and root nodulation of the tropical legume Sesbania rostrata. JOURNAL OF BIOLOGICAL CHEMISTRY, 275(21), 15676–15684.
Vancouver
1.
D’Haeze W, Mergaert P, Promé J-C, Holsters M. Nod factor requirements for efficient stem and root nodulation of the tropical legume Sesbania rostrata. JOURNAL OF BIOLOGICAL CHEMISTRY. 2000;275(21):15676–84.
MLA
D’Haeze, Wim, Peter Mergaert, Jean-Claude Promé, et al. “Nod Factor Requirements for Efficient Stem and Root Nodulation of the Tropical Legume Sesbania Rostrata.” JOURNAL OF BIOLOGICAL CHEMISTRY 275.21 (2000): 15676–15684. Print.