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Expression of cell cycle regulatory genes and morphological alterations in response to salt stress in Arabidopsis thaliana

Sylvia Burssens (UGent) , Kristiina Himanen (UGent) , Brigitte Van De Cotte (UGent) , Tom Beeckman (UGent) , Marc Van Montagu (UGent) , Dirk Inzé (UGent) and Nathalie Verbruggen (UGent)
(2000) PLANTA. 211(5). p.632-640
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Abstract
Hyperosmotic stress severely affects plant growth and development. To examine the effect of salt stress on cell cycle activity in Arabidopsis thaliana (L.) Heynh., the transcriptional regulation of a cyclin-dependent kinase, CDC2aAt, and two mitotic cyclins, Arath;CycB1;1 and Arath;CycA2;1, was studied by using the P-glucuronidase (gus) reporter gene. Moreover, the mRNA abundance of these cell cycle genes as well as CDC2bAt were monitored during salt stress, Upon NaCl treatment, the promoter activities and transcript levels of all cell cycle genes diminished initially in the shoot apex and were subsequently induced during salt-stress adaptation. Additionally, the promoter activities of CDC2aAt and CycA2;1 decreased in the vascular cylinder of the root in correlation with reduced lateral root formation. In the root tips, a regression of CDC2aAt, CycA2;1, and CycB1;1:gus expression was observed, concomitant with a shrinkage of the root meristem and inhibition of root growth. Our data indicate that salt stress interferes with cell cycle regulation at the transcriptional level, resulting in an adaptive growth response.
Keywords
cyclin-dependent kinases, Arabidopsis (cell cycle), beta-glucuronidase, mitotic cyclins, salt stress, WATER-STRESS, SHOOT MERISTEM, ABSCISIC-ACID, OSMOTIC-STRESS, ROOT, DIVISION, KINASE, GROWTH, MAIZE, NUMBER

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Chicago
Burssens, Sylvia, Kristiina Himanen, Brigitte Van De Cotte, Tom Beeckman, Marc Van Montagu, Dirk Inzé, and Nathalie Verbruggen. 2000. “Expression of Cell Cycle Regulatory Genes and Morphological Alterations in Response to Salt Stress in Arabidopsis Thaliana.” Planta 211 (5): 632–640.
APA
Burssens, S., Himanen, K., Van De Cotte, B., Beeckman, T., Van Montagu, M., Inzé, D., & Verbruggen, N. (2000). Expression of cell cycle regulatory genes and morphological alterations in response to salt stress in Arabidopsis thaliana. PLANTA, 211(5), 632–640.
Vancouver
1.
Burssens S, Himanen K, Van De Cotte B, Beeckman T, Van Montagu M, Inzé D, et al. Expression of cell cycle regulatory genes and morphological alterations in response to salt stress in Arabidopsis thaliana. PLANTA. 2000;211(5):632–40.
MLA
Burssens, Sylvia, Kristiina Himanen, Brigitte Van De Cotte, et al. “Expression of Cell Cycle Regulatory Genes and Morphological Alterations in Response to Salt Stress in Arabidopsis Thaliana.” PLANTA 211.5 (2000): 632–640. Print.
@article{124863,
  abstract     = {Hyperosmotic stress severely affects plant growth and development. To examine the effect of salt stress on cell cycle activity in Arabidopsis thaliana (L.) Heynh., the transcriptional regulation of a cyclin-dependent kinase, CDC2aAt, and two mitotic cyclins, Arath;CycB1;1 and Arath;CycA2;1, was studied by using the P-glucuronidase (gus) reporter gene. Moreover, the mRNA abundance of these cell cycle genes as well as CDC2bAt were monitored during salt stress, Upon NaCl treatment, the promoter activities and transcript levels of all cell cycle genes diminished initially in the shoot apex and were subsequently induced during salt-stress adaptation. Additionally, the promoter activities of CDC2aAt and CycA2;1 decreased in the vascular cylinder of the root in correlation with reduced lateral root formation. In the root tips, a regression of CDC2aAt, CycA2;1, and CycB1;1:gus expression was observed, concomitant with a shrinkage of the root meristem and inhibition of root growth. Our data indicate that salt stress interferes with cell cycle regulation at the transcriptional level, resulting in an adaptive growth response.},
  author       = {Burssens, Sylvia and Himanen, Kristiina and Van De Cotte, Brigitte and Beeckman, Tom and Van Montagu, Marc and Inz{\'e}, Dirk and Verbruggen, Nathalie},
  issn         = {0032-0935},
  journal      = {PLANTA},
  keyword      = {cyclin-dependent kinases,Arabidopsis (cell cycle),beta-glucuronidase,mitotic cyclins,salt stress,WATER-STRESS,SHOOT MERISTEM,ABSCISIC-ACID,OSMOTIC-STRESS,ROOT,DIVISION,KINASE,GROWTH,MAIZE,NUMBER},
  language     = {eng},
  number       = {5},
  pages        = {632--640},
  title        = {Expression of cell cycle regulatory genes and morphological alterations in response to salt stress in Arabidopsis thaliana},
  url          = {http://dx.doi.org/10.1007/s004250000334},
  volume       = {211},
  year         = {2000},
}

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