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Embedding thin plant specimens for oriented sectioning

Tom Beeckman (UGent) and Ronnie Viane (UGent)
(2000) BIOTECHNIC & HISTOCHEMISTRY. 75(1). p.23-26
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Abstract
Small plant structures such as small primary roots, filamentous mosses and algae are difficult to orient for sectioning since they become wavy and curl during embedding. A method is described for embedding and orienting tiny plant specimens in a glycol methacrylate resin using self-constructed flat molds. Prior to sectioning, small samples can be oriented in both the longitudinal and the transverse plane. As several samples can be sectioned simultaneously, time-consuming trimming of the blocks is reduced substantially. The efficiency of this technique has been demonstrated using the tiny roots of the model plant Arabidopsis thaliana (L.) Heynh.
Keywords
embedding, Arabidopsis root, orientation, sectioning, plant tissue

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Citation

Please use this url to cite or link to this publication:

MLA
Beeckman, Tom, and Ronnie Viane. “Embedding Thin Plant Specimens for Oriented Sectioning.” BIOTECHNIC & HISTOCHEMISTRY 75.1 (2000): 23–26. Print.
APA
Beeckman, Tom, & Viane, R. (2000). Embedding thin plant specimens for oriented sectioning. BIOTECHNIC & HISTOCHEMISTRY, 75(1), 23–26.
Chicago author-date
Beeckman, Tom, and Ronnie Viane. 2000. “Embedding Thin Plant Specimens for Oriented Sectioning.” Biotechnic & Histochemistry 75 (1): 23–26.
Chicago author-date (all authors)
Beeckman, Tom, and Ronnie Viane. 2000. “Embedding Thin Plant Specimens for Oriented Sectioning.” Biotechnic & Histochemistry 75 (1): 23–26.
Vancouver
1.
Beeckman T, Viane R. Embedding thin plant specimens for oriented sectioning. BIOTECHNIC & HISTOCHEMISTRY. 2000;75(1):23–6.
IEEE
[1]
T. Beeckman and R. Viane, “Embedding thin plant specimens for oriented sectioning,” BIOTECHNIC & HISTOCHEMISTRY, vol. 75, no. 1, pp. 23–26, 2000.
@article{124845,
  abstract     = {Small plant structures such as small primary roots, filamentous mosses and algae are difficult to orient for sectioning since they become wavy and curl during embedding. A method is described for embedding and orienting tiny plant specimens in a glycol methacrylate resin using self-constructed flat molds. Prior to sectioning, small samples can be oriented in both the longitudinal and the transverse plane. As several samples can be sectioned simultaneously, time-consuming trimming of the blocks is reduced substantially. The efficiency of this technique has been demonstrated using the tiny roots of the model plant Arabidopsis thaliana (L.) Heynh.},
  author       = {Beeckman, Tom and Viane, Ronnie},
  issn         = {1052-0295},
  journal      = {BIOTECHNIC & HISTOCHEMISTRY},
  keywords     = {embedding,Arabidopsis root,orientation,sectioning,plant tissue},
  language     = {eng},
  number       = {1},
  pages        = {23--26},
  title        = {Embedding thin plant specimens for oriented sectioning},
  url          = {http://dx.doi.org/10.3109/10520290009047981},
  volume       = {75},
  year         = {2000},
}

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