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Genetic diversity of microcystis blooms (Cyanobacteria) in recently constructed reservoirs in Tigray (Northern Ethiopia) assessed by rDNA ITS

Ineke van Gremberghe, Katleen Van Der Gucht, Pieter Vanormelingen, Tsehaye Asmelash, Tadesse Dejenie, Sofie D'hont, Steven Declerck, Luc De Meester and Wim Vyverman UGent (2011) AQUATIC ECOLOGY. 45(2). p.289-306
abstract
The cyanobacterium Microcystis is notorious for forming extensive and potentially toxic blooms in nutrient-rich freshwater bodies worldwide. However, little is known about the factors underlying the genetic diversity and structure of natural Microcystis populations, despite the fact that this knowledge is essential to understand the build-up of blooms. Microcystis blooms are common and occur year-round in Africa, but are underinvestigated in this continent. We studied the genetic diversity and structure of Microcystis populations in 30 man-made reservoirs in Tigray (Northern Ethiopia) using Denaturing Gradient Gel Electrophoresis of the 16S-23S rDNA internal transcribed spacer (ITS) region and assessed the importance of local environmental conditions and geographic position of the reservoirs for the observed patterns. The analyses showed that both regional and local Microcystis ITS diversity in these recently constructed reservoirs was relatively low, with several dense blooms containing only a single ITS type. Especially one non-toxic ITS type dominated a considerable fraction of Microcystis blooms, but appeared restricted in its geographic distribution. The relationship between Microcystis ITS population structure and abiotic variables (water clarity, pH) and with zooplankton (Daphnia biomass) indicates a (limited) influence of environmental conditions on Microcystis population structure in the reservoirs of Tigray.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
RIBOSOMAL-RNA, SPATIOTEMPORAL CHANGES, FRESH-WATER LAKES, GRADIENT GEL-ELECTROPHORESIS, TOXIC CYANOBACTERIUM, NATURAL-POPULATIONS, INTERGENIC SPACER, NONTOXIC STRAINS, AERUGINOSA, COMMUNITIES, Microcystis, Blooms, 16S-23S rDNA ITS, Population structure, Diversity, Africa
journal title
AQUATIC ECOLOGY
Aquat. Ecol.
volume
45
issue
2
pages
289 - 306
Web of Science type
Article
Web of Science id
000289300200012
JCR category
LIMNOLOGY
JCR impact factor
1.365 (2011)
JCR rank
9/19 (2011)
JCR quartile
2 (2011)
ISSN
1386-2588
DOI
10.1007/s10452-011-9354-z
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1245642
handle
http://hdl.handle.net/1854/LU-1245642
date created
2011-05-27 14:25:28
date last changed
2016-12-19 15:42:50
@article{1245642,
  abstract     = {The cyanobacterium Microcystis is notorious for forming extensive and potentially toxic blooms in nutrient-rich freshwater bodies worldwide. However, little is known about the factors underlying the genetic diversity and structure of natural Microcystis populations, despite the fact that this knowledge is essential to understand the build-up of blooms. Microcystis blooms are common and occur year-round in Africa, but are underinvestigated in this continent. We studied the genetic diversity and structure of Microcystis populations in 30 man-made reservoirs in Tigray (Northern Ethiopia) using Denaturing Gradient Gel Electrophoresis of the 16S-23S rDNA internal transcribed spacer (ITS) region and assessed the importance of local environmental conditions and geographic position of the reservoirs for the observed patterns. The analyses showed that both regional and local Microcystis ITS diversity in these recently constructed reservoirs was relatively low, with several dense blooms containing only a single ITS type. Especially one non-toxic ITS type dominated a considerable fraction of Microcystis blooms, but appeared restricted in its geographic distribution. The relationship between Microcystis ITS population structure and abiotic variables (water clarity, pH) and with zooplankton (Daphnia biomass) indicates a (limited) influence of environmental conditions on Microcystis population structure in the reservoirs of Tigray.},
  author       = {van Gremberghe, Ineke and Van Der Gucht, Katleen and Vanormelingen, Pieter and Asmelash, Tsehaye and Dejenie, Tadesse and D'hont, Sofie and Declerck, Steven and De Meester, Luc and Vyverman, Wim},
  issn         = {1386-2588},
  journal      = {AQUATIC ECOLOGY},
  keyword      = {RIBOSOMAL-RNA,SPATIOTEMPORAL CHANGES,FRESH-WATER LAKES,GRADIENT GEL-ELECTROPHORESIS,TOXIC CYANOBACTERIUM,NATURAL-POPULATIONS,INTERGENIC SPACER,NONTOXIC STRAINS,AERUGINOSA,COMMUNITIES,Microcystis,Blooms,16S-23S rDNA ITS,Population structure,Diversity,Africa},
  language     = {eng},
  number       = {2},
  pages        = {289--306},
  title        = {Genetic diversity of microcystis blooms (Cyanobacteria) in recently constructed reservoirs in Tigray (Northern Ethiopia) assessed by rDNA ITS},
  url          = {http://dx.doi.org/10.1007/s10452-011-9354-z},
  volume       = {45},
  year         = {2011},
}

Chicago
van Gremberghe, Ineke, Katleen Van Der Gucht, Pieter Vanormelingen, Tsehaye Asmelash, Tadesse Dejenie, SOFIE D’HONT, Steven Declerck, Luc De Meester, and Wim Vyverman. 2011. “Genetic Diversity of Microcystis Blooms (Cyanobacteria) in Recently Constructed Reservoirs in Tigray (Northern Ethiopia) Assessed by rDNA ITS.” Aquatic Ecology 45 (2): 289–306.
APA
van Gremberghe, I., Van Der Gucht, K., Vanormelingen, P., Asmelash, T., Dejenie, T., D’HONT, S., Declerck, S., et al. (2011). Genetic diversity of microcystis blooms (Cyanobacteria) in recently constructed reservoirs in Tigray (Northern Ethiopia) assessed by rDNA ITS. AQUATIC ECOLOGY, 45(2), 289–306.
Vancouver
1.
van Gremberghe I, Van Der Gucht K, Vanormelingen P, Asmelash T, Dejenie T, D’HONT S, et al. Genetic diversity of microcystis blooms (Cyanobacteria) in recently constructed reservoirs in Tigray (Northern Ethiopia) assessed by rDNA ITS. AQUATIC ECOLOGY. 2011;45(2):289–306.
MLA
van Gremberghe, Ineke, Katleen Van Der Gucht, Pieter Vanormelingen, et al. “Genetic Diversity of Microcystis Blooms (Cyanobacteria) in Recently Constructed Reservoirs in Tigray (Northern Ethiopia) Assessed by rDNA ITS.” AQUATIC ECOLOGY 45.2 (2011): 289–306. Print.