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Effect of hypoxia in mice mesenteric arteries surrounded by adipose tissue

Nele Maenhaut UGent and Johan Van de Voorde UGent (2011) ACTA PHYSIOLOGICA. 203(1). p.235-244
abstract
Aim: To investigate the influence of hypoxia on the vasoactive effect of perivascular white adipose tissue. Methods: Isometric tension recordings were performed on mesenteric arteries from Swiss male mice with or without adherent adipose tissue. Results: Hypoxia (bubbling with 95% N(2), 5% CO(2)) induced a biphasic response, i.e. vasoconstriction followed by vasorelaxation, in pre-contracted (noradrenaline, 10 mu M) mesenteric arteries with adipose tissue in the presence of indomethacin (10 mu M) and N(omega)-nitro-L-arginine (0.1 mM). Only a small vasorelaxation was observed in arteries without adipose tissue. Pre-contraction with 60 or 120 mm K(+), incubation with tetraethylammoniumchloride (1 and 3 mm), apamin (1 mu M) combined with charybdotoxin (0.1 mu M) or 1-[(2-chlorophenyl) diphenylmethyl]-1H-pyrazole (TRAM-34) (10 mu M) significantly impaired the hypoxic vasorelaxation. Removal of the endothelium only reduced the hypoxic vasorelaxation. Apamin (1 mu M) and charybdotoxin (0.1 mu M) did not influence the vasorelaxation of sodium hydrosulfide hydrate. Zinc protoporphyrin IX (10 mu M), miconazole (10 mu M), 8-(p-sulfophenyl) theophylline (0.1 mm), 1 H-[1, 2, 4] oxadiazolo[4,3-A]quinoxalin-1-one (10 mu M), apocynin (0.3 mM), diphenyliodonium (1 mu M), catalase (2500 U mL(-1)) and tempol (0.1 mM) did not influence the hypoxic vasorelaxation. In contrast to losartan (0.1 mM), indomethacin (10 mu M) and SQ-29548 (10 mu M) significantly reduced the hypoxic vasoconstriction. Conclusions: Moderate hypoxia induces a biphasic vasomotor response in mice mesenteric arteries surrounded by adipose tissue. The hypoxic vasoconstriction is endothelium independent, whereas the vasodilation is endothelium dependent, soluble guanylyl cyclase independent and in part mediated by opening K(Ca) channels. Cyclooxygenase metabolites mediate the hypoxic vasoconstriction, while endothelium-derived hyperpolarizing factor plays a small role in the hypoxic vasorelaxation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
adipose tissue, adipokines, arteries, EDHF, hypoxia, ENDOTHELIUM-DEPENDENT HYPERPOLARIZATION, HYDROGEN-PEROXIDE, SMALL-CONDUCTANCE, CARBON-MONOXIDE, ANGIOTENSIN-II, INFLAMMATION, CHANNELS, VASOCONSTRICTION, DYSFUNCTION, ADIPOCYTES
journal title
ACTA PHYSIOLOGICA
Acta Physiol.
volume
203
issue
1
issue title
18th Acta Physiologica international symposium 'Vascular neuroeffector mechanisms'
pages
235 - 244
Web of Science type
Article
Web of Science id
000293921400021
JCR category
PHYSIOLOGY
JCR impact factor
3.09 (2011)
JCR rank
25/78 (2011)
JCR quartile
2 (2011)
ISSN
1748-1708
DOI
10.1111/j.1748-1716.2010.02238.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1245131
handle
http://hdl.handle.net/1854/LU-1245131
date created
2011-05-27 10:03:31
date last changed
2016-12-19 15:44:47
@article{1245131,
  abstract     = {Aim: To investigate the influence of hypoxia on the vasoactive effect of perivascular white adipose tissue. 
Methods: Isometric tension recordings were performed on mesenteric arteries from Swiss male mice with or without adherent adipose tissue. 
Results: Hypoxia (bubbling with 95\% N(2), 5\% CO(2)) induced a biphasic response, i.e. vasoconstriction followed by vasorelaxation, in pre-contracted (noradrenaline, 10 mu M) mesenteric arteries with adipose tissue in the presence of indomethacin (10 mu M) and N(omega)-nitro-L-arginine (0.1 mM). Only a small vasorelaxation was observed in arteries without adipose tissue. Pre-contraction with 60 or 120 mm K(+), incubation with tetraethylammoniumchloride (1 and 3 mm), apamin (1 mu M) combined with charybdotoxin (0.1 mu M) or 1-[(2-chlorophenyl) diphenylmethyl]-1H-pyrazole (TRAM-34) (10 mu M) significantly impaired the hypoxic vasorelaxation. Removal of the endothelium only reduced the hypoxic vasorelaxation. Apamin (1 mu M) and charybdotoxin (0.1 mu M) did not influence the vasorelaxation of sodium hydrosulfide hydrate. Zinc protoporphyrin IX (10 mu M), miconazole (10 mu M), 8-(p-sulfophenyl) theophylline (0.1 mm), 1 H-[1, 2, 4] oxadiazolo[4,3-A]quinoxalin-1-one (10 mu M), apocynin (0.3 mM), diphenyliodonium (1 mu M), catalase (2500 U mL(-1)) and tempol (0.1 mM) did not influence the hypoxic vasorelaxation. In contrast to losartan (0.1 mM), indomethacin (10 mu M) and SQ-29548 (10 mu M) significantly reduced the hypoxic vasoconstriction. 
Conclusions: Moderate hypoxia induces a biphasic vasomotor response in mice mesenteric arteries surrounded by adipose tissue. The hypoxic vasoconstriction is endothelium independent, whereas the vasodilation is endothelium dependent, soluble guanylyl cyclase independent and in part mediated by opening K(Ca) channels. Cyclooxygenase metabolites mediate the hypoxic vasoconstriction, while endothelium-derived hyperpolarizing factor plays a small role in the hypoxic vasorelaxation.},
  author       = {Maenhaut, Nele and Van de Voorde, Johan},
  issn         = {1748-1708},
  journal      = {ACTA PHYSIOLOGICA},
  keyword      = {adipose tissue,adipokines,arteries,EDHF,hypoxia,ENDOTHELIUM-DEPENDENT HYPERPOLARIZATION,HYDROGEN-PEROXIDE,SMALL-CONDUCTANCE,CARBON-MONOXIDE,ANGIOTENSIN-II,INFLAMMATION,CHANNELS,VASOCONSTRICTION,DYSFUNCTION,ADIPOCYTES},
  language     = {eng},
  number       = {1},
  pages        = {235--244},
  title        = {Effect of hypoxia in mice mesenteric arteries surrounded by adipose tissue},
  url          = {http://dx.doi.org/10.1111/j.1748-1716.2010.02238.x},
  volume       = {203},
  year         = {2011},
}

Chicago
Maenhaut, Nele, and Johan Van de Voorde. 2011. “Effect of Hypoxia in Mice Mesenteric Arteries Surrounded by Adipose Tissue.” Acta Physiologica 203 (1): 235–244.
APA
Maenhaut, N., & Van de Voorde, J. (2011). Effect of hypoxia in mice mesenteric arteries surrounded by adipose tissue. ACTA PHYSIOLOGICA, 203(1), 235–244.
Vancouver
1.
Maenhaut N, Van de Voorde J. Effect of hypoxia in mice mesenteric arteries surrounded by adipose tissue. ACTA PHYSIOLOGICA. 2011;203(1):235–44.
MLA
Maenhaut, Nele, and Johan Van de Voorde. “Effect of Hypoxia in Mice Mesenteric Arteries Surrounded by Adipose Tissue.” ACTA PHYSIOLOGICA 203.1 (2011): 235–244. Print.