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Transformation of banana (Musa spp.) with a D-type cyclin gene from Arabidopsis thaliana (ArathCYCD2;1)

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Abstract
Bananas (Musa spp.), which constitute a staple food in the Great Lakes region of east and central Africa, are characterized by a long growth cycle of 17-22 months. This long cropping cycle affects the crop's productivity per unit time and if reduced has the potential to increase yield. However, lack of early maturity genes in the Musa germplasm and the biology of banana impede the application of conventional breeding for this trait. In this study, we investigated the genetic transformation strategy of over-expressing D-type cyclin genes that have been shown to shorten cell cycle duration and increase growth in model plant species. Using the Agrobacterium tumefaciens transformation technique, embryogenic cells of banana cv. 'Sukalindiizi' (AAB) were transformed with the Arabidopsis thaliana D2;1 gene under the control of maize ubiquitin promoter and neomycin phosphotransferase (nptII) as the selectable marker gene. Selection of the transformed cells on neomycin supplemented medium gave a regeneration frequency of 15% and transformation frequency of 46.8%. PCR, Southern blot and reverse transcriptase PCR analyses on the regenerated shoots confirmed the presence, integration and transcription of the transgene, respectively. Preliminary growth evaluation of glasshouse-potted regenerants identified improved leaf elongation in two lines. Detailed morphological analysis of the transgenic lines is ongoing.
Keywords
growth, genetic transformation, Musa, cyclin

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Chicago
Talengera, D, Gerrit Beemster, Fabio Fiorani, Dirk Inzé, Karl Kunert, and W Tushemereirwe. 2010. “Transformation of Banana (Musa Spp.) with a D-type Cyclin Gene from Arabidopsis Thaliana (ArathCYCD2;1).” In Aspects of Applied Biology, ed. Toby Bruce, Christine Foyer, Nigel Halford, Alfred Keys, Karl Kunert, David Lawlor, Martin Parry, and Graham Russell, 96:45–53.
APA
Talengera, D., Beemster, G., Fiorani, F., Inzé, D., Kunert, K., & Tushemereirwe, W. (2010). Transformation of banana (Musa spp.) with a D-type cyclin gene from Arabidopsis thaliana (ArathCYCD2;1). In T. Bruce, C. Foyer, N. Halford, A. Keys, K. Kunert, D. Lawlor, M. Parry, et al. (Eds.), ASPECTS OF APPLIED BIOLOGY (Vol. 96, pp. 45–53). Presented at the Agriculture : Africa’s “engine for growth” : plant science and biotechnology hold the key.
Vancouver
1.
Talengera D, Beemster G, Fiorani F, Inzé D, Kunert K, Tushemereirwe W. Transformation of banana (Musa spp.) with a D-type cyclin gene from Arabidopsis thaliana (ArathCYCD2;1). In: Bruce T, Foyer C, Halford N, Keys A, Kunert K, Lawlor D, et al., editors. ASPECTS OF APPLIED BIOLOGY. 2010. p. 45–53.
MLA
Talengera, D, Gerrit Beemster, Fabio Fiorani, et al. “Transformation of Banana (Musa Spp.) with a D-type Cyclin Gene from Arabidopsis Thaliana (ArathCYCD2;1).” Aspects of Applied Biology. Ed. Toby Bruce et al. Vol. 96. 2010. 45–53. Print.
@inproceedings{1243192,
  abstract     = {Bananas (Musa spp.), which constitute a staple food in the Great Lakes region of east and central Africa, are characterized by a long growth cycle of 17-22 months. This long cropping cycle affects the crop's productivity per unit time and if reduced has the potential to increase yield. However, lack of early maturity genes in the Musa germplasm and the biology of banana impede the application of conventional breeding for this trait. In this study, we investigated the genetic transformation strategy of over-expressing D-type cyclin genes that have been shown to shorten cell cycle duration and increase growth in model plant species. Using the Agrobacterium tumefaciens transformation technique, embryogenic cells of banana cv. 'Sukalindiizi' (AAB) were transformed with the Arabidopsis thaliana D2;1 gene under the control of maize ubiquitin promoter and neomycin phosphotransferase (nptII) as the selectable marker gene. Selection of the transformed cells on neomycin supplemented medium gave a regeneration frequency of 15\% and transformation frequency of 46.8\%. PCR, Southern blot and reverse transcriptase PCR analyses on the regenerated shoots confirmed the presence, integration and transcription of the transgene, respectively. Preliminary growth evaluation of glasshouse-potted regenerants identified improved leaf elongation in two lines. Detailed morphological analysis of the transgenic lines is ongoing.},
  author       = {Talengera, D and Beemster, Gerrit and Fiorani, Fabio and Inz{\'e}, Dirk and Kunert, Karl and Tushemereirwe, W},
  booktitle    = {ASPECTS OF APPLIED BIOLOGY},
  editor       = {Bruce, Toby and Foyer, Christine and Halford, Nigel and Keys, Alfred and Kunert, Karl and Lawlor, David and Parry, Martin and Russell, Graham},
  issn         = {0265-1491},
  keyword      = {growth,genetic transformation,Musa,cyclin},
  language     = {eng},
  location     = {Harpenden, UK},
  pages        = {45--53},
  title        = {Transformation of banana (Musa spp.) with a D-type cyclin gene from Arabidopsis thaliana (ArathCYCD2;1)},
  volume       = {96},
  year         = {2010},
}