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Mesorhizobium australicum sp. nov. and Mesorhizobium opportunistum sp. nov., isolated from Biserrula pelecinus L. in Australia

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Abstract
Biserrula pelecinus L. is a pasture legume that was introduced to Australia from the Mediterranean basin in 1993. Although the native rhizobial population could not nodulate B. pelecinus at the time of its introduction, recent research has shown the emergence of a diversity of strains (novel isolates) that are able to do so. Three novel isolates, WSM2073(T), WSM2074 and WSM2076, had nearly identical 16S rRNA gene sequences, and clustered separately with all recognized species of the genus Mesorhizobium. Conversely, the novel isolate WSM2075(T) had >23 nt mismatches with the above three isolates. All four novel isolates shared 97-99% 16S rRNA gene sequence similarity with the type strains of all recognized Mesorhizobium species. However, strains WSM2073(T), WSM2074 and WSM2076 showed <95.2% dnaK gene sequence similarity to the type strains of recognized Mesorhizobium species, and <92.9% to WSM2075(T) (which also shared <95.5% dnaK gene sequence similarity to the type strains of recognized Mesorhizobium species). Results for GSll gene sequencing were consistent with those for the dnaK gene. The fatty acid profiles of the novel isolates were diagnostic of root-nodule bacteria, but did not match those of recognized bacterial species. Strain WSM2075(T) had a significantly different fatty acid profile from the other three isolates. The above results indicated that strains WSM2073(T), WSM2074 and WSM2076 represent the same species. Strain WSM2073(T) showed <45% DNA-DNA relatedness and WSM2075(T) <50% DNA-DNA relatedness with the type strains of recognized Mesorhizobium species; these two novel isolates shared 59% DNA-DNA relatedness. Collectively, these data indicate that strains WSM2073(T), WSM2074 and WSM2076, and strain WSM2075(T) belong to two novel species of the genus Mesorhizobium, for which the names Mesorhizobium, australicum sp. nov. and Mesorhizobium opportunistum sp. nov. are proposed, respectively. The type strain of Mesorhizobium australicum sp. nov. is WSM2073(T) (=LMG 24608(T)=HAMBI 3006(T)) and the type strain of Mesorhizobium opportunistum sp. nov. is WSM2075(T) (=LMG 24607(T)=HAMBI 3007(T)).
Keywords
BACTERIAL STRAINS, CICER-ARIETINUM L, HUAKUII, DIVERSITY, EVOLUTION, LEGUMES, LATERAL TRANSFER, LOTI, HYBRIDIZATION, RHIZOBIUM-MEDITERRANEUM

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Citation

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Chicago
Nandasena, Kemanthi G, Graham W O’Hara, Ravi P Tiwari, Anne Willems, and John G Howieson. 2009. “Mesorhizobium Australicum Sp. Nov. and Mesorhizobium Opportunistum Sp. Nov., Isolated from Biserrula Pelecinus L. in Australia.” International Journal of Systematic and Evolutionary Microbiology 59 (9): 2140–2147.
APA
Nandasena, K. G., O’Hara, G. W., Tiwari, R. P., Willems, A., & Howieson, J. G. (2009). Mesorhizobium australicum sp. nov. and Mesorhizobium opportunistum sp. nov., isolated from Biserrula pelecinus L. in Australia. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 59(9), 2140–2147.
Vancouver
1.
Nandasena KG, O’Hara GW, Tiwari RP, Willems A, Howieson JG. Mesorhizobium australicum sp. nov. and Mesorhizobium opportunistum sp. nov., isolated from Biserrula pelecinus L. in Australia. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2009;59(9):2140–7.
MLA
Nandasena, Kemanthi G, Graham W O’Hara, Ravi P Tiwari, et al. “Mesorhizobium Australicum Sp. Nov. and Mesorhizobium Opportunistum Sp. Nov., Isolated from Biserrula Pelecinus L. in Australia.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 59.9 (2009): 2140–2147. Print.
@article{1243163,
  abstract     = {Biserrula pelecinus L. is a pasture legume that was introduced to Australia from the Mediterranean basin in 1993. Although the native rhizobial population could not nodulate B. pelecinus at the time of its introduction, recent research has shown the emergence of a diversity of strains (novel isolates) that are able to do so. Three novel isolates, WSM2073(T), WSM2074 and WSM2076, had nearly identical 16S rRNA gene sequences, and clustered separately with all recognized species of the genus Mesorhizobium. Conversely, the novel isolate WSM2075(T) had >23 nt mismatches with the above three isolates. All four novel isolates shared 97-99% 16S rRNA gene sequence similarity with the type strains of all recognized Mesorhizobium species. However, strains WSM2073(T), WSM2074 and WSM2076 showed <95.2% dnaK gene sequence similarity to the type strains of recognized Mesorhizobium species, and <92.9% to WSM2075(T) (which also shared <95.5% dnaK gene sequence similarity to the type strains of recognized Mesorhizobium species). Results for GSll gene sequencing were consistent with those for the dnaK gene. The fatty acid profiles of the novel isolates were diagnostic of root-nodule bacteria, but did not match those of recognized bacterial species. Strain WSM2075(T) had a significantly different fatty acid profile from the other three isolates. The above results indicated that strains WSM2073(T), WSM2074 and WSM2076 represent the same species. Strain WSM2073(T) showed <45% DNA-DNA relatedness and WSM2075(T) <50% DNA-DNA relatedness with the type strains of recognized Mesorhizobium species; these two novel isolates shared 59% DNA-DNA relatedness. Collectively, these data indicate that strains WSM2073(T), WSM2074 and WSM2076, and strain WSM2075(T) belong to two novel species of the genus Mesorhizobium, for which the names Mesorhizobium, australicum sp. nov. and Mesorhizobium opportunistum sp. nov. are proposed, respectively. The type strain of Mesorhizobium australicum sp. nov. is WSM2073(T) (=LMG 24608(T)=HAMBI 3006(T)) and the type strain of Mesorhizobium opportunistum sp. nov. is WSM2075(T) (=LMG 24607(T)=HAMBI 3007(T)).},
  author       = {Nandasena, Kemanthi G and O'Hara, Graham W and Tiwari, Ravi P and Willems, Anne and Howieson, John G},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keywords     = {BACTERIAL STRAINS,CICER-ARIETINUM L,HUAKUII,DIVERSITY,EVOLUTION,LEGUMES,LATERAL TRANSFER,LOTI,HYBRIDIZATION,RHIZOBIUM-MEDITERRANEUM},
  language     = {eng},
  number       = {9},
  pages        = {2140--2147},
  title        = {Mesorhizobium australicum sp. nov. and Mesorhizobium opportunistum sp. nov., isolated from Biserrula pelecinus L. in Australia},
  url          = {http://dx.doi.org/10.1099/ijs.0.005728-0},
  volume       = {59},
  year         = {2009},
}

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