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Mucosal tissue polyclonal IgE is functional in response to allergen and SEB

Nan Zhang (UGent) , Gabriële Holtappels (UGent) , Philippe Gevaert (UGent) , Joke Patou (UGent) , B Dhaliwal, Hannah Gould and Claus Bachert (UGent)
(2011) ALLERGY. 66(1). p.141-148
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Abstract
Staphylococcus aureus may modify airway disease by inducing local formation of polyclonal IgE antibodies (abs), the role of which is unknown. Methods: Nasal mucosal tissue and serum was obtained from 12 allergic rhinitis (AR) and 14 nasal polyp (NP) subjects. Skin prick tests were performed, and total and specific IgE abs to inhalant allergens and enterotoxin B were determined in serum and tissue. Tissue fragments were stimulated with anti-IgE, enterotoxin B, or grass and house dust mite allergens in different concentrations for 30 min. RBL SX38 cells were sensitized with NP homogenates containing IgE and stimulated with grass pollen extracts. Results: In AR patients, degranulation of tissue mast cells upon allergen exposure and presence of specific IgE to inhalant allergens corresponded in almost all cases. Total IgE concentrations in serum and mucosal tissue homogenates highly correlated. In contrast, in NP patients, reactivity of tissue mast cells upon allergen exposure and presence of specific IgE to inhalant allergens or Staphylococcus aureus enterotoxin B corresponded for tissue, but not for serum. Total IgE was significantly higher in tissue compared to serum and failed to show correlation. Tissue IgE to grass pollen was functional to degranulate RBL cells. Conclusion: We here demonstrate that mucosal IgE abs in NP tissue are functional and able to activate mast cells; specific IgE abs in NP tissue can be found independently of their presence in serum. We postulate that superantigen-induced polyclonal IgE in airway disease contributes to chronic inflammation by continuously activating mast cells.
Keywords
EXOTOXINS, STAPHYLOCOCCUS-AUREUS ENTEROTOXINS, BINDING, NASAL POLYPS, SURVIVAL, AFFINITY, superantigens, polyclonal, nasal polyps, mast cells, IgE

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Chicago
Zhang, Nan, Gabriële Holtappels, Philippe Gevaert, Joke Patou, B Dhaliwal, Hannah Gould, and Claus Bachert. 2011. “Mucosal Tissue Polyclonal IgE Is Functional in Response to Allergen and SEB.” Allergy 66 (1): 141–148.
APA
Zhang, Nan, Holtappels, G., Gevaert, P., Patou, J., Dhaliwal, B., Gould, H., & Bachert, C. (2011). Mucosal tissue polyclonal IgE is functional in response to allergen and SEB. ALLERGY, 66(1), 141–148.
Vancouver
1.
Zhang N, Holtappels G, Gevaert P, Patou J, Dhaliwal B, Gould H, et al. Mucosal tissue polyclonal IgE is functional in response to allergen and SEB. ALLERGY. 2011;66(1):141–8.
MLA
Zhang, Nan, Gabriële Holtappels, Philippe Gevaert, et al. “Mucosal Tissue Polyclonal IgE Is Functional in Response to Allergen and SEB.” ALLERGY 66.1 (2011): 141–148. Print.
@article{1242832,
  abstract     = {Staphylococcus aureus may modify airway disease by inducing local formation of polyclonal IgE antibodies (abs), the role of which is unknown. Methods: Nasal mucosal tissue and serum was obtained from 12 allergic rhinitis (AR) and 14 nasal polyp (NP) subjects. Skin prick tests were performed, and total and specific IgE abs to inhalant allergens and enterotoxin B were determined in serum and tissue. Tissue fragments were stimulated with anti-IgE, enterotoxin B, or grass and house dust mite allergens in different concentrations for 30 min. RBL SX38 cells were sensitized with NP homogenates containing IgE and stimulated with grass pollen extracts. Results: In AR patients, degranulation of tissue mast cells upon allergen exposure and presence of specific IgE to inhalant allergens corresponded in almost all cases. Total IgE concentrations in serum and mucosal tissue homogenates highly correlated. In contrast, in NP patients, reactivity of tissue mast cells upon allergen exposure and presence of specific IgE to inhalant allergens or Staphylococcus aureus enterotoxin B corresponded for tissue, but not for serum. Total IgE was significantly higher in tissue compared to serum and failed to show correlation. Tissue IgE to grass pollen was functional to degranulate RBL cells. Conclusion: We here demonstrate that mucosal IgE abs in NP tissue are functional and able to activate mast cells; specific IgE abs in NP tissue can be found independently of their presence in serum. We postulate that superantigen-induced polyclonal IgE in airway disease contributes to chronic inflammation by continuously activating mast cells.},
  author       = {Zhang, Nan and Holtappels, Gabri{\"e}le and Gevaert, Philippe and Patou, Joke and Dhaliwal, B and Gould, Hannah and Bachert, Claus},
  issn         = {0105-4538},
  journal      = {ALLERGY},
  language     = {eng},
  number       = {1},
  pages        = {141--148},
  title        = {Mucosal tissue polyclonal IgE is functional in response to allergen and SEB},
  url          = {http://dx.doi.org/10.1111/j.1398-9995.2010.02448.x},
  volume       = {66},
  year         = {2011},
}

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