Ghent University Academic Bibliography

Advanced

Gravity-induced PIN transcytosis for polarization of auxin fluxes in gravity-sensing root cells

Jürgen Kleine-Vehn UGent, Zhaojun Ding UGent, Angharad Jones UGent, Masao Tasaka, Miyo T Morita and Jiri Friml UGent (2010) PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 107(51). p.22344-22349
abstract
Auxin is an essential plant-specific regulator of patterning processes that also controls directional growth of roots and shoots. In response to gravity stimulation, the PIN3 auxin transporter polarizes to the bottom side of gravity-sensing root cells, presumably redirecting the auxin flux toward the lower side of the root and triggering gravitropic bending. By combining live-cell imaging techniques with pharmacological and genetic approaches, we demonstrate that PIN3 polarization does not require secretion of de novo synthesized proteins or protein degradation, but instead involves rapid, transient stimulation of PIN endocytosis, presumably via a clathrin-dependent pathway. Moreover, gravity-induced PIN3 polarization requires the activity of the guanine nucleotide exchange factors for ARF GTPases (ARF-GEF) GNOM-dependent polar-targeting pathways and might involve endosome-based PIN3 translocation from one cell side to another. Our data suggest that gravity perception acts at several instances of PIN3 trafficking, ultimately leading to the polarization of PIN3, which presumably aligns auxin fluxes with gravity vector and mediates downstream root gravitropic response.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PROTEINS, ARABIDOPSIS, EFFLUX CARRIER, PLANT DEVELOPMENT, TRANSPORT, EXPRESSION, GRADIENTS, POLARITY, GNOM, GRAVITROPISM, polarity, exocytosis, gravitropism, plant cell biology
journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Proc. Natl. Acad. Sci. U. S. A.
volume
107
issue
51
pages
22344 - 22349
Web of Science type
Article
Web of Science id
000285521800074
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
9.771 (2010)
JCR rank
3/56 (2010)
JCR quartile
1 (2010)
ISSN
0027-8424
DOI
10.1073/pnas.1013145107
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1242674
handle
http://hdl.handle.net/1854/LU-1242674
date created
2011-05-25 15:58:25
date last changed
2012-09-19 14:03:50
@article{1242674,
  abstract     = {Auxin is an essential plant-specific regulator of patterning processes that also controls directional growth of roots and shoots. In response to gravity stimulation, the PIN3 auxin transporter polarizes to the bottom side of gravity-sensing root cells, presumably redirecting the auxin flux toward the lower side of the root and triggering gravitropic bending. By combining live-cell imaging techniques with pharmacological and genetic approaches, we demonstrate that PIN3 polarization does not require secretion of de novo synthesized proteins or protein degradation, but instead involves rapid, transient stimulation of PIN endocytosis, presumably via a clathrin-dependent pathway. Moreover, gravity-induced PIN3 polarization requires the activity of the guanine nucleotide exchange factors for ARF GTPases (ARF-GEF) GNOM-dependent polar-targeting pathways and might involve endosome-based PIN3 translocation from one cell side to another. Our data suggest that gravity perception acts at several instances of PIN3 trafficking, ultimately leading to the polarization of PIN3, which presumably aligns auxin fluxes with gravity vector and mediates downstream root gravitropic response.},
  author       = {Kleine-Vehn, J{\"u}rgen and Ding, Zhaojun and Jones, Angharad and Tasaka, Masao and Morita, Miyo T and Friml, Jiri},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keyword      = {PROTEINS,ARABIDOPSIS,EFFLUX CARRIER,PLANT DEVELOPMENT,TRANSPORT,EXPRESSION,GRADIENTS,POLARITY,GNOM,GRAVITROPISM,polarity,exocytosis,gravitropism,plant cell biology},
  language     = {eng},
  number       = {51},
  pages        = {22344--22349},
  title        = {Gravity-induced PIN transcytosis for polarization of auxin fluxes in gravity-sensing root cells},
  url          = {http://dx.doi.org/10.1073/pnas.1013145107},
  volume       = {107},
  year         = {2010},
}

Chicago
Kleine-Vehn, Jürgen, Zhaojun Ding, Angharad Jones, Masao Tasaka, Miyo T Morita, and Jiri Friml. 2010. “Gravity-induced PIN Transcytosis for Polarization of Auxin Fluxes in Gravity-sensing Root Cells.” Proceedings of the National Academy of Sciences of the United States of America 107 (51): 22344–22349.
APA
Kleine-Vehn, J., Ding, Z., Jones, A., Tasaka, M., Morita, M. T., & Friml, J. (2010). Gravity-induced PIN transcytosis for polarization of auxin fluxes in gravity-sensing root cells. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 107(51), 22344–22349.
Vancouver
1.
Kleine-Vehn J, Ding Z, Jones A, Tasaka M, Morita MT, Friml J. Gravity-induced PIN transcytosis for polarization of auxin fluxes in gravity-sensing root cells. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2010;107(51):22344–9.
MLA
Kleine-Vehn, Jürgen, Zhaojun Ding, Angharad Jones, et al. “Gravity-induced PIN Transcytosis for Polarization of Auxin Fluxes in Gravity-sensing Root Cells.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 107.51 (2010): 22344–22349. Print.