Advanced search
1 file | 1.30 MB

Gravity-induced PIN transcytosis for polarization of auxin fluxes in gravity-sensing root cells

Author
Organization
Abstract
Auxin is an essential plant-specific regulator of patterning processes that also controls directional growth of roots and shoots. In response to gravity stimulation, the PIN3 auxin transporter polarizes to the bottom side of gravity-sensing root cells, presumably redirecting the auxin flux toward the lower side of the root and triggering gravitropic bending. By combining live-cell imaging techniques with pharmacological and genetic approaches, we demonstrate that PIN3 polarization does not require secretion of de novo synthesized proteins or protein degradation, but instead involves rapid, transient stimulation of PIN endocytosis, presumably via a clathrin-dependent pathway. Moreover, gravity-induced PIN3 polarization requires the activity of the guanine nucleotide exchange factors for ARF GTPases (ARF-GEF) GNOM-dependent polar-targeting pathways and might involve endosome-based PIN3 translocation from one cell side to another. Our data suggest that gravity perception acts at several instances of PIN3 trafficking, ultimately leading to the polarization of PIN3, which presumably aligns auxin fluxes with gravity vector and mediates downstream root gravitropic response.
Keywords
PROTEINS, ARABIDOPSIS, EFFLUX CARRIER, PLANT DEVELOPMENT, TRANSPORT, EXPRESSION, GRADIENTS, POLARITY, GNOM, GRAVITROPISM, polarity, exocytosis, gravitropism, plant cell biology

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.30 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Kleine-Vehn, Jürgen, Zhaojun Ding, Angharad Jones, Masao Tasaka, Miyo T Morita, and Jiri Friml. 2010. “Gravity-induced PIN Transcytosis for Polarization of Auxin Fluxes in Gravity-sensing Root Cells.” Proceedings of the National Academy of Sciences of the United States of America 107 (51): 22344–22349.
APA
Kleine-Vehn, J., Ding, Z., Jones, A., Tasaka, M., Morita, M. T., & Friml, J. (2010). Gravity-induced PIN transcytosis for polarization of auxin fluxes in gravity-sensing root cells. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 107(51), 22344–22349.
Vancouver
1.
Kleine-Vehn J, Ding Z, Jones A, Tasaka M, Morita MT, Friml J. Gravity-induced PIN transcytosis for polarization of auxin fluxes in gravity-sensing root cells. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2010;107(51):22344–9.
MLA
Kleine-Vehn, Jürgen, Zhaojun Ding, Angharad Jones, et al. “Gravity-induced PIN Transcytosis for Polarization of Auxin Fluxes in Gravity-sensing Root Cells.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 107.51 (2010): 22344–22349. Print.
@article{1242674,
  abstract     = {Auxin is an essential plant-specific regulator of patterning processes that also controls directional growth of roots and shoots. In response to gravity stimulation, the PIN3 auxin transporter polarizes to the bottom side of gravity-sensing root cells, presumably redirecting the auxin flux toward the lower side of the root and triggering gravitropic bending. By combining live-cell imaging techniques with pharmacological and genetic approaches, we demonstrate that PIN3 polarization does not require secretion of de novo synthesized proteins or protein degradation, but instead involves rapid, transient stimulation of PIN endocytosis, presumably via a clathrin-dependent pathway. Moreover, gravity-induced PIN3 polarization requires the activity of the guanine nucleotide exchange factors for ARF GTPases (ARF-GEF) GNOM-dependent polar-targeting pathways and might involve endosome-based PIN3 translocation from one cell side to another. Our data suggest that gravity perception acts at several instances of PIN3 trafficking, ultimately leading to the polarization of PIN3, which presumably aligns auxin fluxes with gravity vector and mediates downstream root gravitropic response.},
  author       = {Kleine-Vehn, J{\"u}rgen and Ding, Zhaojun and Jones, Angharad and Tasaka, Masao and Morita, Miyo T and Friml, Jiri},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keyword      = {PROTEINS,ARABIDOPSIS,EFFLUX CARRIER,PLANT DEVELOPMENT,TRANSPORT,EXPRESSION,GRADIENTS,POLARITY,GNOM,GRAVITROPISM,polarity,exocytosis,gravitropism,plant cell biology},
  language     = {eng},
  number       = {51},
  pages        = {22344--22349},
  title        = {Gravity-induced PIN transcytosis for polarization of auxin fluxes in gravity-sensing root cells},
  url          = {http://dx.doi.org/10.1073/pnas.1013145107},
  volume       = {107},
  year         = {2010},
}

Altmetric
View in Altmetric
Web of Science
Times cited: