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T cell inflammatory response, Foxp3 and TNFRS18-L regulation of peripheral blood mononuclear cells from patients with nasal polyps-asthma after staphylococcal superantigen stimulation

Claudina Perez Novo UGent, Monica Jedrzejczak-Czechowicz, A Lewandowska-Polak, Cindy Claeys UGent, Gabriële Holtappels UGent, Paul Van Cauwenberge UGent, ML Kowalski and Claus Bachert UGent (2010) CLINICAL AND EXPERIMENTAL ALLERGY. 40(9). p.1323-1332
abstract
Background Staphylococcal superantigens may modulate airway inflammatory disease. Objective We assessed the effect of Staphylococcus aureus enterotoxin B (SEB) on T cell activation in patients with nasal polyps and asthma, and its possible link to aspirin hypersensitivity. Methods Leucocytes were isolated from five healthy subjects (controls), five asthmatics with nasal polyps without (NP-ATA) and five with aspirin-induced asthma (NP-AIA). Cells were incubated with increasing concentrations of SEB for 4 and 18 h. Release of T(H)1/T(H)2 cytokines was assessed by Cytometric Bead-Array. Foxp3 and TNFRS18-L expression were analysed by qPCR and flow cytometry. Results After 4 and 18 h, SEB significantly increased IFN-gamma, IL-4, TNF-alpha, IL-5 and IL-2 concentrations in supernatants of both NP polyp groups compared with controls. Baseline Foxp3 was significantly decreased in both NP-asthma groups. Incubation with SEB for 4 h induced a limited up-regulation of Foxp3 in NP-AIA patients, which was switched off consecutively. Foxp3 was significantly up-regulated in the control group after 18 h, but not in the NP-asthmatic groups. In parallel, TNFRS18-L mRNA significantly increased after 18 h in the NP-asthma groups compared with control subjects. This molecule was highly expressed in CD11c(+) CD14(+) cells and its levels increased after 18 and 24 h culture in the NP-asthma patients. Conclusion SEB induces both T(H)1 and T(H)2 pro-inflammatory responses in patients with nasal polyps and asthma regardless of the presence of aspirin hypersensitivity. The nature of this response may be linked to a basal deficiency of Foxp3 observed in the NP-asthmatic patients and/or to the up-regulation of TNFRS18-L on monocytes/dendritic cell precursors.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
AUREUS ENTEROTOXINS, INTOLERANT ASTHMA, ASPIRIN-SENSITIVE ASTHMATICS, ALLERGIC-ASTHMA, IGE ANTIBODIES, RHINOSINUSITIS, PROSTAGLANDIN, INSUFFICIENCY, GENERATION, EXPRESSION, aspirin hypersensitivity, Foxp3, nasal polyposis, regulatory T cells, staphylococcal enterotoxin B, T(H)1/T(H)2 cytokines, TNFRS18-L
journal title
CLINICAL AND EXPERIMENTAL ALLERGY
Clin. Exp. Allergy
volume
40
issue
9
pages
1323 - 1332
Web of Science type
Article
Web of Science id
000280651700006
JCR category
ALLERGY
JCR impact factor
4.195 (2010)
JCR rank
3/20 (2010)
JCR quartile
1 (2010)
ISSN
0954-7894
DOI
10.1111/j.1365-2222.2010.03577.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1241911
handle
http://hdl.handle.net/1854/LU-1241911
date created
2011-05-25 14:43:55
date last changed
2011-06-01 13:53:39
@article{1241911,
  abstract     = {Background Staphylococcal superantigens may modulate airway inflammatory disease. Objective We assessed the effect of Staphylococcus aureus enterotoxin B (SEB) on T cell activation in patients with nasal polyps and asthma, and its possible link to aspirin hypersensitivity. Methods Leucocytes were isolated from five healthy subjects (controls), five asthmatics with nasal polyps without (NP-ATA) and five with aspirin-induced asthma (NP-AIA). Cells were incubated with increasing concentrations of SEB for 4 and 18 h. Release of T(H)1/T(H)2 cytokines was assessed by Cytometric Bead-Array. Foxp3 and TNFRS18-L expression were analysed by qPCR and flow cytometry. Results After 4 and 18 h, SEB significantly increased IFN-gamma, IL-4, TNF-alpha, IL-5 and IL-2 concentrations in supernatants of both NP polyp groups compared with controls. Baseline Foxp3 was significantly decreased in both NP-asthma groups. Incubation with SEB for 4 h induced a limited up-regulation of Foxp3 in NP-AIA patients, which was switched off consecutively. Foxp3 was significantly up-regulated in the control group after 18 h, but not in the NP-asthmatic groups. In parallel, TNFRS18-L mRNA significantly increased after 18 h in the NP-asthma groups compared with control subjects. This molecule was highly expressed in CD11c(+) CD14(+) cells and its levels increased after 18 and 24 h culture in the NP-asthma patients. Conclusion SEB induces both T(H)1 and T(H)2 pro-inflammatory responses in patients with nasal polyps and asthma regardless of the presence of aspirin hypersensitivity. The nature of this response may be linked to a basal deficiency of Foxp3 observed in the NP-asthmatic patients and/or to the up-regulation of TNFRS18-L on monocytes/dendritic cell precursors.},
  author       = {Perez Novo, Claudina and Jedrzejczak-Czechowicz, Monica and Lewandowska-Polak, A and Claeys, Cindy and Holtappels, Gabri{\"e}le and Van Cauwenberge, Paul and Kowalski, ML and Bachert, Claus},
  issn         = {0954-7894},
  journal      = {CLINICAL AND EXPERIMENTAL ALLERGY},
  keyword      = {AUREUS ENTEROTOXINS,INTOLERANT ASTHMA,ASPIRIN-SENSITIVE ASTHMATICS,ALLERGIC-ASTHMA,IGE ANTIBODIES,RHINOSINUSITIS,PROSTAGLANDIN,INSUFFICIENCY,GENERATION,EXPRESSION,aspirin hypersensitivity,Foxp3,nasal polyposis,regulatory T cells,staphylococcal enterotoxin B,T(H)1/T(H)2 cytokines,TNFRS18-L},
  language     = {eng},
  number       = {9},
  pages        = {1323--1332},
  title        = {T cell inflammatory response, Foxp3 and TNFRS18-L regulation of peripheral blood mononuclear cells from patients with nasal polyps-asthma after staphylococcal superantigen stimulation},
  url          = {http://dx.doi.org/10.1111/j.1365-2222.2010.03577.x},
  volume       = {40},
  year         = {2010},
}

Chicago
Perez Novo, Claudina, Monica Jedrzejczak-Czechowicz, A Lewandowska-Polak, Cindy Claeys, Gabriële Holtappels, Paul Van Cauwenberge, ML Kowalski, and Claus Bachert. 2010. “T Cell Inflammatory Response, Foxp3 and TNFRS18-L Regulation of Peripheral Blood Mononuclear Cells from Patients with Nasal Polyps-asthma After Staphylococcal Superantigen Stimulation.” Clinical and Experimental Allergy 40 (9): 1323–1332.
APA
Perez Novo, C., Jedrzejczak-Czechowicz, M., Lewandowska-Polak, A., Claeys, C., Holtappels, G., Van Cauwenberge, P., Kowalski, M., et al. (2010). T cell inflammatory response, Foxp3 and TNFRS18-L regulation of peripheral blood mononuclear cells from patients with nasal polyps-asthma after staphylococcal superantigen stimulation. CLINICAL AND EXPERIMENTAL ALLERGY, 40(9), 1323–1332.
Vancouver
1.
Perez Novo C, Jedrzejczak-Czechowicz M, Lewandowska-Polak A, Claeys C, Holtappels G, Van Cauwenberge P, et al. T cell inflammatory response, Foxp3 and TNFRS18-L regulation of peripheral blood mononuclear cells from patients with nasal polyps-asthma after staphylococcal superantigen stimulation. CLINICAL AND EXPERIMENTAL ALLERGY. 2010;40(9):1323–32.
MLA
Perez Novo, Claudina, Monica Jedrzejczak-Czechowicz, A Lewandowska-Polak, et al. “T Cell Inflammatory Response, Foxp3 and TNFRS18-L Regulation of Peripheral Blood Mononuclear Cells from Patients with Nasal Polyps-asthma After Staphylococcal Superantigen Stimulation.” CLINICAL AND EXPERIMENTAL ALLERGY 40.9 (2010): 1323–1332. Print.