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Virulence quenching with a prenylated isoflavanone renders the Malagasy legume Dalbergia pervillei resistant to Rhodococcus fascians

Sanda Rajaonson, Olivier Vandeputte, Danny Vereecke UGent, Martin Kiendrebeogo, Eliane Ralambofetra, Caroline Stévigny, Pierre Duez, Christian Rabemanantsoa, Adeline Mol and Billo Diallo, et al. (2011) ENVIRONMENTAL MICROBIOLOGY. 13(5). p.1236-1252
abstract
The phytopathogenic Actinomycete Rhodococcus fascians induces leafy galls on a wide range of hosts, causing major economical losses in the ornamentals industry. Although differences in the responsivity occur within species, no plant tested so far could be considered resistant to R. fascians strain D188 infection. Here, we observed that members of the genus Dalbergia, which belong to the Fabaceae, did not develop leafy galls when challenged with R. fascians and we set out to unravel the mechanism of this recalcitrance. Whereas organic extracts of Dalbergia tissues exhibited toxicity towards the bacteria, more importantly, dichloromethane bark extracts inhibited the induction of bacterial virulence gene expression without any apparent loss of viability, illustrating that resistance is likely multifactorial. The virulence quencher was identified as a new prenylated isoflavanone, termed perbergin, and specifically targeted the AttR regulon (a LysR-type transcriptional regulator) which is imperative for the switch of R. fascians from an epiphytic to a pathogenic lifestyle. The mode of action of perbergin demonstrated that just like in Gram-negative host-microbe interactions, also in Gram-positive phytopathogens autoregulation is being targeted by the plant as an efficient means of defence. Moreover, the identification of perbergin opens the path to disease control in affected nurseries.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
AZORHIZOBIUM-CAULINODANS, TRANSCRIPTIONAL REGULATOR, HERBACEOUS PERENNIALS, FLAVONOID COMPOUNDS, CHEMICAL DIVERSITY, GENE-EXPRESSION, PLANT-TISSUES, ISOFLAVONOIDS, SOPHORA-TETRAPTERA, LEAFY GALL FORMATION
journal title
ENVIRONMENTAL MICROBIOLOGY
Environ. Microbiol.
volume
13
issue
5
pages
1236 - 1252
Web of Science type
Article
Web of Science id
000289798600011
JCR category
MICROBIOLOGY
JCR impact factor
5.843 (2011)
JCR rank
14/111 (2011)
JCR quartile
1 (2011)
ISSN
1462-2912
DOI
10.1111/j.1462-2920.2011.02424.x
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1241439
handle
http://hdl.handle.net/1854/LU-1241439
date created
2011-05-25 13:41:34
date last changed
2014-03-24 11:28:50
@article{1241439,
  abstract     = {The phytopathogenic Actinomycete Rhodococcus fascians induces leafy galls on a wide range of hosts, causing major economical losses in the ornamentals industry. Although differences in the responsivity occur within species, no plant tested so far could be considered resistant to R. fascians strain D188 infection. Here, we observed that members of the genus Dalbergia, which belong to the Fabaceae, did not develop leafy galls when challenged with R. fascians and we set out to unravel the mechanism of this recalcitrance. Whereas organic extracts of Dalbergia tissues exhibited toxicity towards the bacteria, more importantly, dichloromethane bark extracts inhibited the induction of bacterial virulence gene expression without any apparent loss of viability, illustrating that resistance is likely multifactorial. The virulence quencher was identified as a new prenylated isoflavanone, termed perbergin, and specifically targeted the AttR regulon (a LysR-type transcriptional regulator) which is imperative for the switch of R. fascians from an epiphytic to a pathogenic lifestyle. The mode of action of perbergin demonstrated that just like in Gram-negative host-microbe interactions, also in Gram-positive phytopathogens autoregulation is being targeted by the plant as an efficient means of defence. Moreover, the identification of perbergin opens the path to disease control in affected nurseries.},
  author       = {Rajaonson, Sanda and Vandeputte, Olivier  and Vereecke, Danny and Kiendrebeogo, Martin and Ralambofetra, Eliane and St{\'e}vigny, Caroline and Duez, Pierre and Rabemanantsoa, Christian and Mol, Adeline and Diallo, Billo and Baucher, Marie and El Jaziri, Mondher},
  issn         = {1462-2912},
  journal      = {ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {AZORHIZOBIUM-CAULINODANS,TRANSCRIPTIONAL REGULATOR,HERBACEOUS PERENNIALS,FLAVONOID COMPOUNDS,CHEMICAL DIVERSITY,GENE-EXPRESSION,PLANT-TISSUES,ISOFLAVONOIDS,SOPHORA-TETRAPTERA,LEAFY GALL FORMATION},
  language     = {eng},
  number       = {5},
  pages        = {1236--1252},
  title        = {Virulence quenching with a prenylated isoflavanone renders the Malagasy legume Dalbergia pervillei resistant to Rhodococcus fascians},
  url          = {http://dx.doi.org/10.1111/j.1462-2920.2011.02424.x},
  volume       = {13},
  year         = {2011},
}

Chicago
Rajaonson, Sanda, Olivier Vandeputte, Danny Vereecke, Martin Kiendrebeogo, Eliane Ralambofetra, Caroline Stévigny, Pierre Duez, et al. 2011. “Virulence Quenching with a Prenylated Isoflavanone Renders the Malagasy Legume Dalbergia Pervillei Resistant to Rhodococcus Fascians.” Environmental Microbiology 13 (5): 1236–1252.
APA
Rajaonson, S., Vandeputte, O., Vereecke, D., Kiendrebeogo, M., Ralambofetra, E., Stévigny, C., Duez, P., et al. (2011). Virulence quenching with a prenylated isoflavanone renders the Malagasy legume Dalbergia pervillei resistant to Rhodococcus fascians. ENVIRONMENTAL MICROBIOLOGY, 13(5), 1236–1252.
Vancouver
1.
Rajaonson S, Vandeputte O, Vereecke D, Kiendrebeogo M, Ralambofetra E, Stévigny C, et al. Virulence quenching with a prenylated isoflavanone renders the Malagasy legume Dalbergia pervillei resistant to Rhodococcus fascians. ENVIRONMENTAL MICROBIOLOGY. 2011;13(5):1236–52.
MLA
Rajaonson, Sanda, Olivier Vandeputte, Danny Vereecke, et al. “Virulence Quenching with a Prenylated Isoflavanone Renders the Malagasy Legume Dalbergia Pervillei Resistant to Rhodococcus Fascians.” ENVIRONMENTAL MICROBIOLOGY 13.5 (2011): 1236–1252. Print.