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Virulence quenching with a prenylated isoflavanone renders the Malagasy legume Dalbergia pervillei resistant to Rhodococcus fascians

(2011) ENVIRONMENTAL MICROBIOLOGY. 13(5). p.1236-1252
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Abstract
The phytopathogenic Actinomycete Rhodococcus fascians induces leafy galls on a wide range of hosts, causing major economical losses in the ornamentals industry. Although differences in the responsivity occur within species, no plant tested so far could be considered resistant to R. fascians strain D188 infection. Here, we observed that members of the genus Dalbergia, which belong to the Fabaceae, did not develop leafy galls when challenged with R. fascians and we set out to unravel the mechanism of this recalcitrance. Whereas organic extracts of Dalbergia tissues exhibited toxicity towards the bacteria, more importantly, dichloromethane bark extracts inhibited the induction of bacterial virulence gene expression without any apparent loss of viability, illustrating that resistance is likely multifactorial. The virulence quencher was identified as a new prenylated isoflavanone, termed perbergin, and specifically targeted the AttR regulon (a LysR-type transcriptional regulator) which is imperative for the switch of R. fascians from an epiphytic to a pathogenic lifestyle. The mode of action of perbergin demonstrated that just like in Gram-negative host-microbe interactions, also in Gram-positive phytopathogens autoregulation is being targeted by the plant as an efficient means of defence. Moreover, the identification of perbergin opens the path to disease control in affected nurseries.
Keywords
AZORHIZOBIUM-CAULINODANS, TRANSCRIPTIONAL REGULATOR, HERBACEOUS PERENNIALS, FLAVONOID COMPOUNDS, CHEMICAL DIVERSITY, GENE-EXPRESSION, PLANT-TISSUES, ISOFLAVONOIDS, SOPHORA-TETRAPTERA, LEAFY GALL FORMATION

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Citation

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Chicago
Rajaonson, Sanda, Olivier Vandeputte, Danny Vereecke, Martin Kiendrebeogo, Eliane Ralambofetra, Caroline Stévigny, Pierre Duez, et al. 2011. “Virulence Quenching with a Prenylated Isoflavanone Renders the Malagasy Legume Dalbergia Pervillei Resistant to Rhodococcus Fascians.” Environmental Microbiology 13 (5): 1236–1252.
APA
Rajaonson, S., Vandeputte, O., Vereecke, D., Kiendrebeogo, M., Ralambofetra, E., Stévigny, C., Duez, P., et al. (2011). Virulence quenching with a prenylated isoflavanone renders the Malagasy legume Dalbergia pervillei resistant to Rhodococcus fascians. ENVIRONMENTAL MICROBIOLOGY, 13(5), 1236–1252.
Vancouver
1.
Rajaonson S, Vandeputte O, Vereecke D, Kiendrebeogo M, Ralambofetra E, Stévigny C, et al. Virulence quenching with a prenylated isoflavanone renders the Malagasy legume Dalbergia pervillei resistant to Rhodococcus fascians. ENVIRONMENTAL MICROBIOLOGY. 2011;13(5):1236–52.
MLA
Rajaonson, Sanda, Olivier Vandeputte, Danny Vereecke, et al. “Virulence Quenching with a Prenylated Isoflavanone Renders the Malagasy Legume Dalbergia Pervillei Resistant to Rhodococcus Fascians.” ENVIRONMENTAL MICROBIOLOGY 13.5 (2011): 1236–1252. Print.
@article{1241439,
  abstract     = {The phytopathogenic Actinomycete Rhodococcus fascians induces leafy galls on a wide range of hosts, causing major economical losses in the ornamentals industry. Although differences in the responsivity occur within species, no plant tested so far could be considered resistant to R. fascians strain D188 infection. Here, we observed that members of the genus Dalbergia, which belong to the Fabaceae, did not develop leafy galls when challenged with R. fascians and we set out to unravel the mechanism of this recalcitrance. Whereas organic extracts of Dalbergia tissues exhibited toxicity towards the bacteria, more importantly, dichloromethane bark extracts inhibited the induction of bacterial virulence gene expression without any apparent loss of viability, illustrating that resistance is likely multifactorial. The virulence quencher was identified as a new prenylated isoflavanone, termed perbergin, and specifically targeted the AttR regulon (a LysR-type transcriptional regulator) which is imperative for the switch of R. fascians from an epiphytic to a pathogenic lifestyle. The mode of action of perbergin demonstrated that just like in Gram-negative host-microbe interactions, also in Gram-positive phytopathogens autoregulation is being targeted by the plant as an efficient means of defence. Moreover, the identification of perbergin opens the path to disease control in affected nurseries.},
  author       = {Rajaonson, Sanda and Vandeputte, Olivier  and Vereecke, Danny and Kiendrebeogo, Martin and Ralambofetra, Eliane and St{\'e}vigny, Caroline and Duez, Pierre and Rabemanantsoa, Christian and Mol, Adeline and Diallo, Billo and Baucher, Marie and El Jaziri, Mondher},
  issn         = {1462-2912},
  journal      = {ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {AZORHIZOBIUM-CAULINODANS,TRANSCRIPTIONAL REGULATOR,HERBACEOUS PERENNIALS,FLAVONOID COMPOUNDS,CHEMICAL DIVERSITY,GENE-EXPRESSION,PLANT-TISSUES,ISOFLAVONOIDS,SOPHORA-TETRAPTERA,LEAFY GALL FORMATION},
  language     = {eng},
  number       = {5},
  pages        = {1236--1252},
  title        = {Virulence quenching with a prenylated isoflavanone renders the Malagasy legume Dalbergia pervillei resistant to Rhodococcus fascians},
  url          = {http://dx.doi.org/10.1111/j.1462-2920.2011.02424.x},
  volume       = {13},
  year         = {2011},
}

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