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Polar-localized NPH3-like proteins regulate polarity and endocytosis of PIN-FORMED auxin efflux carriers

Masahiko Furutani, Norihito Sakamoto, Shuhei Yoshida, Takahito Kajiwara, Helene Boisivon UGent, Jiri Friml UGent and Masao Tasaka (2011) DEVELOPMENT. 138(10). p.2069-2078
abstract
PIN-FORMED (PIN)-dependent auxin transport is essential for plant development and its modulation in response to the environment or endogenous signals. A NON-PHOTOTROPIC HYPOCOTYL 3 (NPH3)-like protein, MACCHI-BOU 4 (MAB4), has been shown to control PIN1 localization during organ formation, but its contribution is limited. The Arabidopsis genome contains four genes, MAB4/ENP/NPY1-LIKE1 (MEL1), MEL2, MEL3 and MEL4, highly homologous to MAB4. Genetic analysis disclosed functional redundancy between MAB4 and MEL genes in regulation of not only organ formation but also of root gravitropism, revealing that NPH3 family proteins have a wider range of functions than previously suspected. Multiple mutants showed severe reduction in PIN abundance and PIN polar localization, leading to defective expression of an auxin responsive marker DR5rev::GFP. Pharmacological analyses and fluorescence recovery after photo-bleaching experiments showed that mel mutations increase PIN2 internalization from the plasma membrane, but affect neither intracellular PIN2 trafficking nor PIN2 lateral diffusion at the plasma membrane. Notably, all MAB4 subfamily proteins show polar localization at the cell periphery in plants. The MAB4 polarity was almost identical to PIN polarity. Our results suggest that the MAB4 subfamily proteins specifically retain PIN proteins in a polarized manner at the plasma membrane, thus controlling directional auxin transport and plant development.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Arabidopsis, GNOM ARF-GEF, Auxin, Endocytosis, PIN, ARABIDOPSIS-THALIANA, DEPENDENT ENDOCYTOSIS, COTYLEDON DEVELOPMENT, REVERSE GENETICS, PLASMA-MEMBRANE, CELL POLARITY, TRANSPORT, ORGANOGENESIS, PHOTOTROPISM
journal title
DEVELOPMENT
Development
volume
138
issue
10
pages
2069 - 2078
Web of Science type
Article
Web of Science id
000289886200018
JCR category
DEVELOPMENTAL BIOLOGY
JCR impact factor
6.596 (2011)
JCR rank
5/40 (2011)
JCR quartile
1 (2011)
ISSN
0950-1991
DOI
10.1242/dev.057745
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1241407
handle
http://hdl.handle.net/1854/LU-1241407
date created
2011-05-25 13:41:11
date last changed
2011-06-08 11:32:22
@article{1241407,
  abstract     = {PIN-FORMED (PIN)-dependent auxin transport is essential for plant development and its modulation in response to the environment or endogenous signals. A NON-PHOTOTROPIC HYPOCOTYL 3 (NPH3)-like protein, MACCHI-BOU 4 (MAB4), has been shown to control PIN1 localization during organ formation, but its contribution is limited. The Arabidopsis genome contains four genes, MAB4/ENP/NPY1-LIKE1 (MEL1), MEL2, MEL3 and MEL4, highly homologous to MAB4. Genetic analysis disclosed functional redundancy between MAB4 and MEL genes in regulation of not only organ formation but also of root gravitropism, revealing that NPH3 family proteins have a wider range of functions than previously suspected. Multiple mutants showed severe reduction in PIN abundance and PIN polar localization, leading to defective expression of an auxin responsive marker DR5rev::GFP. Pharmacological analyses and fluorescence recovery after photo-bleaching experiments showed that mel mutations increase PIN2 internalization from the plasma membrane, but affect neither intracellular PIN2 trafficking nor PIN2 lateral diffusion at the plasma membrane. Notably, all MAB4 subfamily proteins show polar localization at the cell periphery in plants. The MAB4 polarity was almost identical to PIN polarity. Our results suggest that the MAB4 subfamily proteins specifically retain PIN proteins in a polarized manner at the plasma membrane, thus controlling directional auxin transport and plant development.},
  author       = {Furutani, Masahiko and Sakamoto, Norihito and Yoshida, Shuhei and Kajiwara, Takahito and Boisivon, Helene and Friml, Jiri and Tasaka, Masao},
  issn         = {0950-1991},
  journal      = {DEVELOPMENT},
  keyword      = {Arabidopsis,GNOM ARF-GEF,Auxin,Endocytosis,PIN,ARABIDOPSIS-THALIANA,DEPENDENT ENDOCYTOSIS,COTYLEDON DEVELOPMENT,REVERSE GENETICS,PLASMA-MEMBRANE,CELL POLARITY,TRANSPORT,ORGANOGENESIS,PHOTOTROPISM},
  language     = {eng},
  number       = {10},
  pages        = {2069--2078},
  title        = {Polar-localized NPH3-like proteins regulate polarity and endocytosis of PIN-FORMED auxin efflux carriers},
  url          = {http://dx.doi.org/10.1242/dev.057745},
  volume       = {138},
  year         = {2011},
}

Chicago
Furutani, Masahiko, Norihito Sakamoto, Shuhei Yoshida, Takahito Kajiwara, Helene Boisivon, Jiri Friml, and Masao Tasaka. 2011. “Polar-localized NPH3-like Proteins Regulate Polarity and Endocytosis of PIN-FORMED Auxin Efflux Carriers.” Development 138 (10): 2069–2078.
APA
Furutani, M., Sakamoto, N., Yoshida, S., Kajiwara, T., Boisivon, H., Friml, J., & Tasaka, M. (2011). Polar-localized NPH3-like proteins regulate polarity and endocytosis of PIN-FORMED auxin efflux carriers. DEVELOPMENT, 138(10), 2069–2078.
Vancouver
1.
Furutani M, Sakamoto N, Yoshida S, Kajiwara T, Boisivon H, Friml J, et al. Polar-localized NPH3-like proteins regulate polarity and endocytosis of PIN-FORMED auxin efflux carriers. DEVELOPMENT. 2011;138(10):2069–78.
MLA
Furutani, Masahiko, Norihito Sakamoto, Shuhei Yoshida, et al. “Polar-localized NPH3-like Proteins Regulate Polarity and Endocytosis of PIN-FORMED Auxin Efflux Carriers.” DEVELOPMENT 138.10 (2011): 2069–2078. Print.