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Genetic characterization of African swine fever viruses from a 2008 outbreak in Tanzania

G Misinzo, J Magambo, J Masambu, MG Yongolo, Joris Van Doorsselaere and Hans Nauwynck UGent (2011) TRANSBOUNDARY AND EMERGING DISEASES. 58(1). p.86-92
abstract
Outbreaks of African swine fever (ASF) have been reported in the past from several countries in sub-Saharan Africa. The aim of this study was to genotype ASF viruses (ASFVs) from the 2008 outbreak in Morogoro and Dar es Salaam regions of Tanzania. Tissue samples from domestic pigs that died as a result of severe haemorrhagic disease were collected and analysed with PCR and genome sequencing methods using ASFV-specific primer sets. Nucleotide sequence data were obtained for the B646L (p72), E183L (p54) and the variable region of the B602L gene sequences. Phylogenetic analyses based on DNA sequences showed that the 2008 Tanzanian isolates belonged to p72 genotype XV and clustered together with those derived from the 2001 outbreak in Tanzania. Analysis of the tetrameric amino acid repeat regions within the variable region of the B602L gene showed that the repeat signature of the 2008 Tanzanian ASFV was unique and contained three novel tetramers (U = NIDT/NTDT and X = NTDI). Epidemiological investigation suggested that transportation of live pigs continues to play an active role in the epidemiology of ASF in Tanzania. It is recommended that future control of ASF spread in Tanzania should focus on the early detection and confirmation of the disease, prompt institution of quarantine measures, culling and proper disposal of infected and in-contact animals and decontamination of affected premises.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Asfarviridae, P72, arbovirus, domestic pigs, genotyping, phylogenetic analysis, African swine fever outbreaks, DIAGNOSIS, GENOME SEQUENCES, MOLECULAR EPIDEMIOLOGY
journal title
TRANSBOUNDARY AND EMERGING DISEASES
Transbound. Emerg. Dis.
volume
58
issue
1
pages
86 - 92
Web of Science type
Article
Web of Science id
000286059900010
JCR category
VETERINARY SCIENCES
JCR impact factor
1.809 (2011)
JCR rank
22/141 (2011)
JCR quartile
1 (2011)
ISSN
1865-1674
DOI
10.1111/j.1865-1682.2010.01177.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1235752
handle
http://hdl.handle.net/1854/LU-1235752
date created
2011-05-25 10:20:55
date last changed
2016-12-19 15:44:49
@article{1235752,
  abstract     = {Outbreaks of African swine fever (ASF) have been reported in the past from several countries in sub-Saharan Africa. The aim of this study was to genotype ASF viruses (ASFVs) from the 2008 outbreak in Morogoro and Dar es Salaam regions of Tanzania. Tissue samples from domestic pigs that died as a result of severe haemorrhagic disease were collected and analysed with PCR and genome sequencing methods using ASFV-specific primer sets. Nucleotide sequence data were obtained for the B646L (p72), E183L (p54) and the variable region of the B602L gene sequences. Phylogenetic analyses based on DNA sequences showed that the 2008 Tanzanian isolates belonged to p72 genotype XV and clustered together with those derived from the 2001 outbreak in Tanzania. Analysis of the tetrameric amino acid repeat regions within the variable region of the B602L gene showed that the repeat signature of the 2008 Tanzanian ASFV was unique and contained three novel tetramers (U = NIDT/NTDT and X = NTDI). Epidemiological investigation suggested that transportation of live pigs continues to play an active role in the epidemiology of ASF in Tanzania. It is recommended that future control of ASF spread in Tanzania should focus on the early detection and confirmation of the disease, prompt institution of quarantine measures, culling and proper disposal of infected and in-contact animals and decontamination of affected premises.},
  author       = {Misinzo, G and Magambo, J and Masambu, J and Yongolo, MG and Van Doorsselaere, Joris and Nauwynck, Hans},
  issn         = {1865-1674},
  journal      = {TRANSBOUNDARY AND EMERGING DISEASES},
  keyword      = {Asfarviridae,P72,arbovirus,domestic pigs,genotyping,phylogenetic analysis,African swine fever outbreaks,DIAGNOSIS,GENOME SEQUENCES,MOLECULAR EPIDEMIOLOGY},
  language     = {eng},
  number       = {1},
  pages        = {86--92},
  title        = {Genetic characterization of African swine fever viruses from a 2008 outbreak in Tanzania},
  url          = {http://dx.doi.org/10.1111/j.1865-1682.2010.01177.x},
  volume       = {58},
  year         = {2011},
}

Chicago
Misinzo, G, J Magambo, J Masambu, MG Yongolo, Joris Van Doorsselaere, and Hans Nauwynck. 2011. “Genetic Characterization of African Swine Fever Viruses from a 2008 Outbreak in Tanzania.” Transboundary and Emerging Diseases 58 (1): 86–92.
APA
Misinzo, G, Magambo, J., Masambu, J., Yongolo, M., Van Doorsselaere, J., & Nauwynck, H. (2011). Genetic characterization of African swine fever viruses from a 2008 outbreak in Tanzania. TRANSBOUNDARY AND EMERGING DISEASES, 58(1), 86–92.
Vancouver
1.
Misinzo G, Magambo J, Masambu J, Yongolo M, Van Doorsselaere J, Nauwynck H. Genetic characterization of African swine fever viruses from a 2008 outbreak in Tanzania. TRANSBOUNDARY AND EMERGING DISEASES. 2011;58(1):86–92.
MLA
Misinzo, G, J Magambo, J Masambu, et al. “Genetic Characterization of African Swine Fever Viruses from a 2008 Outbreak in Tanzania.” TRANSBOUNDARY AND EMERGING DISEASES 58.1 (2011): 86–92. Print.