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Genetic characterization of African swine fever viruses from a 2008 outbreak in Tanzania

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Abstract
Outbreaks of African swine fever (ASF) have been reported in the past from several countries in sub-Saharan Africa. The aim of this study was to genotype ASF viruses (ASFVs) from the 2008 outbreak in Morogoro and Dar es Salaam regions of Tanzania. Tissue samples from domestic pigs that died as a result of severe haemorrhagic disease were collected and analysed with PCR and genome sequencing methods using ASFV-specific primer sets. Nucleotide sequence data were obtained for the B646L (p72), E183L (p54) and the variable region of the B602L gene sequences. Phylogenetic analyses based on DNA sequences showed that the 2008 Tanzanian isolates belonged to p72 genotype XV and clustered together with those derived from the 2001 outbreak in Tanzania. Analysis of the tetrameric amino acid repeat regions within the variable region of the B602L gene showed that the repeat signature of the 2008 Tanzanian ASFV was unique and contained three novel tetramers (U = NIDT/NTDT and X = NTDI). Epidemiological investigation suggested that transportation of live pigs continues to play an active role in the epidemiology of ASF in Tanzania. It is recommended that future control of ASF spread in Tanzania should focus on the early detection and confirmation of the disease, prompt institution of quarantine measures, culling and proper disposal of infected and in-contact animals and decontamination of affected premises.
Keywords
Asfarviridae, P72, arbovirus, domestic pigs, genotyping, phylogenetic analysis, African swine fever outbreaks, DIAGNOSIS, GENOME SEQUENCES, MOLECULAR EPIDEMIOLOGY

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Chicago
Misinzo, G, J Magambo, J Masambu, MG Yongolo, Joris Van Doorsselaere, and Hans Nauwynck. 2011. “Genetic Characterization of African Swine Fever Viruses from a 2008 Outbreak in Tanzania.” Transboundary and Emerging Diseases 58 (1): 86–92.
APA
Misinzo, G, Magambo, J., Masambu, J., Yongolo, M., Van Doorsselaere, J., & Nauwynck, H. (2011). Genetic characterization of African swine fever viruses from a 2008 outbreak in Tanzania. TRANSBOUNDARY AND EMERGING DISEASES, 58(1), 86–92.
Vancouver
1.
Misinzo G, Magambo J, Masambu J, Yongolo M, Van Doorsselaere J, Nauwynck H. Genetic characterization of African swine fever viruses from a 2008 outbreak in Tanzania. TRANSBOUNDARY AND EMERGING DISEASES. 2011;58(1):86–92.
MLA
Misinzo, G, J Magambo, J Masambu, et al. “Genetic Characterization of African Swine Fever Viruses from a 2008 Outbreak in Tanzania.” TRANSBOUNDARY AND EMERGING DISEASES 58.1 (2011): 86–92. Print.
@article{1235752,
  abstract     = {Outbreaks of African swine fever (ASF) have been reported in the past from several countries in sub-Saharan Africa. The aim of this study was to genotype ASF viruses (ASFVs) from the 2008 outbreak in Morogoro and Dar es Salaam regions of Tanzania. Tissue samples from domestic pigs that died as a result of severe haemorrhagic disease were collected and analysed with PCR and genome sequencing methods using ASFV-specific primer sets. Nucleotide sequence data were obtained for the B646L (p72), E183L (p54) and the variable region of the B602L gene sequences. Phylogenetic analyses based on DNA sequences showed that the 2008 Tanzanian isolates belonged to p72 genotype XV and clustered together with those derived from the 2001 outbreak in Tanzania. Analysis of the tetrameric amino acid repeat regions within the variable region of the B602L gene showed that the repeat signature of the 2008 Tanzanian ASFV was unique and contained three novel tetramers (U = NIDT/NTDT and X = NTDI). Epidemiological investigation suggested that transportation of live pigs continues to play an active role in the epidemiology of ASF in Tanzania. It is recommended that future control of ASF spread in Tanzania should focus on the early detection and confirmation of the disease, prompt institution of quarantine measures, culling and proper disposal of infected and in-contact animals and decontamination of affected premises.},
  author       = {Misinzo, G and Magambo, J and Masambu, J and Yongolo, MG and Van Doorsselaere, Joris and Nauwynck, Hans},
  issn         = {1865-1674},
  journal      = {TRANSBOUNDARY AND EMERGING DISEASES},
  keyword      = {Asfarviridae,P72,arbovirus,domestic pigs,genotyping,phylogenetic analysis,African swine fever outbreaks,DIAGNOSIS,GENOME SEQUENCES,MOLECULAR EPIDEMIOLOGY},
  language     = {eng},
  number       = {1},
  pages        = {86--92},
  title        = {Genetic characterization of African swine fever viruses from a 2008 outbreak in Tanzania},
  url          = {http://dx.doi.org/10.1111/j.1865-1682.2010.01177.x},
  volume       = {58},
  year         = {2011},
}

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