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Immunizing pigs with Ascaris suum haemoglobin increases the inflammatory response in the liver but fails to induce a protective immunity

Johnny Vlaminck UGent, M Martinez-Valladares, Sylvia Dewilde, Luc Moens, Katrien Tilleman, Dieter Deforce UGent, J Urban, Edwin Claerebout UGent, Jozef Vercruysse UGent and Peter Geldhof UGent (2011) PARASITE IMMUNOLOGY. 33(4). p.250-254
abstract
To determine whether purified Ascaris suum haemoglobin (AsHb) is a suitable vaccine candidate for the control of Ascaris infections, pigs were vaccinated with AsHb in combination with QuilA adjuvant and challenged with A. suum eggs. The number of liver lesions and worms in the intestine was assessed on day 14, 28 and 56 post-infection (p.i.). No significant differences were found in the number of worms recovered between vaccinated and control pigs on any of these days. However, significantly more white spots were counted on the livers of vaccinated pigs on day 14 (+86%) and day 28 (+118%) p.i. compared with nonvaccinated controls. To investigate whether the increased immunoreactivity against the liver stage L3s in vaccinated pigs was triggered by and directed against AsHb, the transcription and expression of AsHb in this larval life stage was analysed by RT-PCR and immunoblotting. The results showed that neither the AsHb transcript nor protein was detectable in freshly hatched L3. However, the immunoblot analysis showed that vaccination with AsHb resulted in the production of antibodies binding to several other antigens of the L3, suggesting that these might be involved in the increased white spot development.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ULTRAVIOLET-ATTENUATED EGGS, INFECTIVE EGGS, Ascaris suum, LARVAE, haemoglobin, vaccination, PROTEIN, white spots, DYNAMICS, MIGRATION, IMMUNIZATION, RESISTANCE, ANTIGENS
journal title
PARASITE IMMUNOLOGY
Parasite Immunol.
volume
33
issue
4
pages
250 - 254
Web of Science type
Article
Web of Science id
000288249400007
JCR category
PARASITOLOGY
JCR impact factor
2.601 (2011)
JCR rank
11/33 (2011)
JCR quartile
2 (2011)
ISSN
0141-9838
DOI
10.1111/j.1365-3024.2010.01274.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1235465
handle
http://hdl.handle.net/1854/LU-1235465
date created
2011-05-25 09:04:26
date last changed
2016-12-19 15:42:48
@article{1235465,
  abstract     = {To determine whether purified Ascaris suum haemoglobin (AsHb) is a suitable vaccine candidate for the control of Ascaris infections, pigs were vaccinated with AsHb in combination with QuilA adjuvant and challenged with A. suum eggs. The number of liver lesions and worms in the intestine was assessed on day 14, 28 and 56 post-infection (p.i.). No significant differences were found in the number of worms recovered between vaccinated and control pigs on any of these days. However, significantly more white spots were counted on the livers of vaccinated pigs on day 14 (+86\%) and day 28 (+118\%) p.i. compared with nonvaccinated controls. To investigate whether the increased immunoreactivity against the liver stage L3s in vaccinated pigs was triggered by and directed against AsHb, the transcription and expression of AsHb in this larval life stage was analysed by RT-PCR and immunoblotting. The results showed that neither the AsHb transcript nor protein was detectable in freshly hatched L3. However, the immunoblot analysis showed that vaccination with AsHb resulted in the production of antibodies binding to several other antigens of the L3, suggesting that these might be involved in the increased white spot development.},
  author       = {Vlaminck, Johnny and Martinez-Valladares, M and Dewilde, Sylvia and Moens, Luc and Tilleman, Katrien and Deforce, Dieter and Urban, J and Claerebout, Edwin and Vercruysse, Jozef and Geldhof, Peter},
  issn         = {0141-9838},
  journal      = {PARASITE IMMUNOLOGY},
  keyword      = {ULTRAVIOLET-ATTENUATED EGGS,INFECTIVE EGGS,Ascaris suum,LARVAE,haemoglobin,vaccination,PROTEIN,white spots,DYNAMICS,MIGRATION,IMMUNIZATION,RESISTANCE,ANTIGENS},
  language     = {eng},
  number       = {4},
  pages        = {250--254},
  title        = {Immunizing pigs with Ascaris suum haemoglobin increases the inflammatory response in the liver but fails to induce a protective immunity},
  url          = {http://dx.doi.org/10.1111/j.1365-3024.2010.01274.x},
  volume       = {33},
  year         = {2011},
}

Chicago
Vlaminck, Johnny, M Martinez-Valladares, Sylvia Dewilde, Luc Moens, Katrien Tilleman, Dieter Deforce, J Urban, Edwin Claerebout, Jozef Vercruysse, and Peter Geldhof. 2011. “Immunizing Pigs with Ascaris Suum Haemoglobin Increases the Inflammatory Response in the Liver but Fails to Induce a Protective Immunity.” Parasite Immunology 33 (4): 250–254.
APA
Vlaminck, J., Martinez-Valladares, M., Dewilde, S., Moens, L., Tilleman, K., Deforce, D., Urban, J., et al. (2011). Immunizing pigs with Ascaris suum haemoglobin increases the inflammatory response in the liver but fails to induce a protective immunity. PARASITE IMMUNOLOGY, 33(4), 250–254.
Vancouver
1.
Vlaminck J, Martinez-Valladares M, Dewilde S, Moens L, Tilleman K, Deforce D, et al. Immunizing pigs with Ascaris suum haemoglobin increases the inflammatory response in the liver but fails to induce a protective immunity. PARASITE IMMUNOLOGY. 2011;33(4):250–4.
MLA
Vlaminck, Johnny, M Martinez-Valladares, Sylvia Dewilde, et al. “Immunizing Pigs with Ascaris Suum Haemoglobin Increases the Inflammatory Response in the Liver but Fails to Induce a Protective Immunity.” PARASITE IMMUNOLOGY 33.4 (2011): 250–254. Print.