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Arcobacter trophiarum sp. nov., isolated from fattening pigs

Sarah De Smet UGent, Peter Vandamme UGent, Lieven De Zutter UGent, Stephen LW On, Laid Douidah UGent and Kurt Houf UGent (2011) INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 61(2). p.356-361
abstract
In the course of a longitudinal study elucidating the dynamics of Arcobacter populations in pigs, 16 isolates of Gram-reaction-negative, rod-shaped, slightly curved, non-spore-forming bacteria were grouped by amplified fragment length polymorphism analysis into a distinct phenon within the genus Arcobacter. Fragments were generated for all isolates in a genus-specific PCR assay, but no amplicon was obtained in a species-specific multiplex-PCR test. Numerical analysis of the whole-cell protein profiles also showed that all isolates clustered in a single group that was distinct from related members of the genus Arcobacter. DNA-DNA hybridizations between two representative strains, designated 64(T) and 122, of the isolates obtained exhibited a mean DNA-DNA relatedness of 72%. DNA-DNA hybridizations between strains 64(T) and 122 and reference strains of other animal-related bacteria of the genus Arcobacter revealed binding values of 47% or less. The DNA G + C contents of the two representative strains were 28.5 and 28.4 mol%, respectively, and analysis of three marker genes identified Arcobacter cryaerophilus, A. thereius, A. cibarius and A. skirrowii as their closest phylogenetic neighbours. Strains 64(T) and 122 could be distinguished from other members of the genus Arcobacter by means of biochemical tests for catalase and urease activities, nitrate reduction, indoxyl acetate hydrolysis, lack of growth at 37 degrees C, growth in 2% (w/v) NaCl, growth on 0.1% sodium deoxycholate and non-supplemented Campylobacter charcoal-deoxycholate base medium and resistance to cephalothin (32 mg l(-1)) and cefoperazone (64 mg l(-1)). Additionally, a PCR assay was developed for the detection and identification of strains 64(T) and 122, which represent a novel species of the genus Arcobacter, for which the name Arcobacter trophiarum sp. nov. is proposed. The type strain is strain 64(T) (=LMG 25534(T) =CCUG 59229(T)).
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PCR, HELICOBACTER, CAMPYLOBACTER, IDENTIFICATION, GENUS ARCOBACTER, PATHOGEN ARCOBACTER, POULTRY PRODUCTS, ORGANISMS, BUTZLERI, SKIRROWII
journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Int. J. Syst. Evol. Microbiol.
volume
61
issue
2
pages
356 - 361
Web of Science type
Article
Web of Science id
000288471300025
JCR category
MICROBIOLOGY
JCR impact factor
2.268 (2011)
JCR rank
63/111 (2011)
JCR quartile
3 (2011)
ISSN
1466-5026
DOI
10.1099/ijs.0.022665-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1235432
handle
http://hdl.handle.net/1854/LU-1235432
date created
2011-05-25 08:47:58
date last changed
2011-05-31 12:18:23
@article{1235432,
  abstract     = {In the course of a longitudinal study elucidating the dynamics of Arcobacter populations in pigs, 16 isolates of Gram-reaction-negative, rod-shaped, slightly curved, non-spore-forming bacteria were grouped by amplified fragment length polymorphism analysis into a distinct phenon within the genus Arcobacter. Fragments were generated for all isolates in a genus-specific PCR assay, but no amplicon was obtained in a species-specific multiplex-PCR test. Numerical analysis of the whole-cell protein profiles also showed that all isolates clustered in a single group that was distinct from related members of the genus Arcobacter. DNA-DNA hybridizations between two representative strains, designated 64(T) and 122, of the isolates obtained exhibited a mean DNA-DNA relatedness of 72\%. DNA-DNA hybridizations between strains 64(T) and 122 and reference strains of other animal-related bacteria of the genus Arcobacter revealed binding values of 47\% or less. The DNA G + C contents of the two representative strains were 28.5 and 28.4 mol\%, respectively, and analysis of three marker genes identified Arcobacter cryaerophilus, A. thereius, A. cibarius and A. skirrowii as their closest phylogenetic neighbours. Strains 64(T) and 122 could be distinguished from other members of the genus Arcobacter by means of biochemical tests for catalase and urease activities, nitrate reduction, indoxyl acetate hydrolysis, lack of growth at 37 degrees C, growth in 2\% (w/v) NaCl, growth on 0.1\% sodium deoxycholate and non-supplemented Campylobacter charcoal-deoxycholate base medium and resistance to cephalothin (32 mg l(-1)) and cefoperazone (64 mg l(-1)). Additionally, a PCR assay was developed for the detection and identification of strains 64(T) and 122, which represent a novel species of the genus Arcobacter, for which the name Arcobacter trophiarum sp. nov. is proposed. The type strain is strain 64(T) (=LMG 25534(T) =CCUG 59229(T)).},
  author       = {De Smet, Sarah and Vandamme, Peter and De Zutter, Lieven and On, Stephen LW and Douidah, Laid and Houf, Kurt},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {PCR,HELICOBACTER,CAMPYLOBACTER,IDENTIFICATION,GENUS ARCOBACTER,PATHOGEN ARCOBACTER,POULTRY PRODUCTS,ORGANISMS,BUTZLERI,SKIRROWII},
  language     = {eng},
  number       = {2},
  pages        = {356--361},
  title        = {Arcobacter trophiarum sp. nov., isolated from fattening pigs},
  url          = {http://dx.doi.org/10.1099/ijs.0.022665-0},
  volume       = {61},
  year         = {2011},
}

Chicago
De Smet, Sarah, Peter Vandamme, Lieven De Zutter, Stephen LW On, Laid Douidah, and Kurt Houf. 2011. “Arcobacter Trophiarum Sp. Nov., Isolated from Fattening Pigs.” International Journal of Systematic and Evolutionary Microbiology 61 (2): 356–361.
APA
De Smet, Sarah, Vandamme, P., De Zutter, L., On, S. L., Douidah, L., & Houf, K. (2011). Arcobacter trophiarum sp. nov., isolated from fattening pigs. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 61(2), 356–361.
Vancouver
1.
De Smet S, Vandamme P, De Zutter L, On SL, Douidah L, Houf K. Arcobacter trophiarum sp. nov., isolated from fattening pigs. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2011;61(2):356–61.
MLA
De Smet, Sarah, Peter Vandamme, Lieven De Zutter, et al. “Arcobacter Trophiarum Sp. Nov., Isolated from Fattening Pigs.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 61.2 (2011): 356–361. Print.