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Multiple insecticide resistance: an impediment to insecticide-based malaria vector control program

Delenasaw Yewhalaw Gebre, Walter Steurbaut, Pieter Spanoghe UGent, Wim Van Bortel, Leen Denis, Dejene A Tessema, Yehenew Getachew Kifle, Marc Coosemans, Luc Duchateau UGent and Niko Speybroeck (2011) PLOS ONE. 6(1).
abstract
Background: Indoor Residual Spraying (IRS), insecticide-treated nets (ITNs) and long-lasting insecticidal nets (LLINs) are key components in malaria prevention and control strategy. However, the development of resistance by mosquitoes to insecticides recommended for IRS and/or ITNs/LLINs would affect insecticide-based malaria vector control. We assessed the susceptibility levels of Anopheles arabiensis to insecticides used in malaria control, characterized basic mechanisms underlying resistance, and evaluated the role of public health use of insecticides in resistance selection. Methodology/Principal findings: Susceptibility status of An. arabiensis was assessed using WHO bioassay tests to DDT, permethrin, deltamethrin, malathion and propoxur in Ethiopia from August to September 2009. Mosquito specimens were screened for knockdown resistance (kdr) and insensitive acetylcholinesterase (ace-1(R)) mutations using AS-PCR and PCR-RFLP, respectively. DDT residues level in soil from human dwellings and the surrounding environment were determined by Gas Chromatography with Electron Capture Detector. An. arabiensis was resistant to DDT, permethrin, deltamethrin and malathion, but susceptible to propoxur. The West African kdr allele was found in 280 specimens out of 284 with a frequency ranged from 95% to 100%. Ace-1(R) mutation was not detected in all specimens scored for the allele. Moreover, DDT residues were found in soil samples from human dwellings but not in the surrounding environment. Conclusion: The observed multiple-resistance coupled with the occurrence of high kdr frequency in populations of An. arabiensis could profoundly affect the malaria vector control programme in Ethiopia. This needs an urgent call for implementing rational resistance management strategies and integrated vector control intervention.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
WEST-AFRICA, KDR MUTATION, KNOCKDOWN RESISTANCE, ANOPHELES-GAMBIAE COMPLEX, CULEX-QUINQUEFASCIATUS, NATURAL-POPULATIONS, MOSQUITO VECTORS, BURKINA-FASO, ARABIENSIS, DDT
journal title
PLOS ONE
PLoS One
volume
6
issue
1
article number
e16066
pages
7 pages
Web of Science type
Article
Web of Science id
000286514300006
JCR category
BIOLOGY
JCR impact factor
4.092 (2011)
JCR rank
12/84 (2011)
JCR quartile
1 (2011)
ISSN
1932-6203
DOI
10.1371/journal.pone.0016066
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1235081
handle
http://hdl.handle.net/1854/LU-1235081
date created
2011-05-24 21:27:10
date last changed
2016-12-21 15:42:20
@article{1235081,
  abstract     = {Background: Indoor Residual Spraying (IRS), insecticide-treated nets (ITNs) and long-lasting insecticidal nets (LLINs) are key components in malaria prevention and control strategy. However, the development of resistance by mosquitoes to insecticides recommended for IRS and/or ITNs/LLINs would affect insecticide-based malaria vector control. We assessed the susceptibility levels of Anopheles arabiensis to insecticides used in malaria control, characterized basic mechanisms underlying resistance, and evaluated the role of public health use of insecticides in resistance selection. Methodology/Principal findings: Susceptibility status of An. arabiensis was assessed using WHO bioassay tests to DDT, permethrin, deltamethrin, malathion and propoxur in Ethiopia from August to September 2009. Mosquito specimens were screened for knockdown resistance (kdr) and insensitive acetylcholinesterase (ace-1(R)) mutations using AS-PCR and PCR-RFLP, respectively. DDT residues level in soil from human dwellings and the surrounding environment were determined by Gas Chromatography with Electron Capture Detector. An. arabiensis was resistant to DDT, permethrin, deltamethrin and malathion, but susceptible to propoxur. The West African kdr allele was found in 280 specimens out of 284 with a frequency ranged from 95\% to 100\%. Ace-1(R) mutation was not detected in all specimens scored for the allele. Moreover, DDT residues were found in soil samples from human dwellings but not in the surrounding environment. Conclusion: The observed multiple-resistance coupled with the occurrence of high kdr frequency in populations of An. arabiensis could profoundly affect the malaria vector control programme in Ethiopia. This needs an urgent call for implementing rational resistance management strategies and integrated vector control intervention.},
  articleno    = {e16066},
  author       = {Gebre, Delenasaw Yewhalaw and Steurbaut, Walter and Spanoghe, Pieter and Van Bortel, Wim and Denis, Leen and Tessema, Dejene A and Kifle, Yehenew Getachew and Coosemans, Marc and Duchateau, Luc and Speybroeck, Niko},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {WEST-AFRICA,KDR MUTATION,KNOCKDOWN RESISTANCE,ANOPHELES-GAMBIAE COMPLEX,CULEX-QUINQUEFASCIATUS,NATURAL-POPULATIONS,MOSQUITO VECTORS,BURKINA-FASO,ARABIENSIS,DDT},
  language     = {eng},
  number       = {1},
  pages        = {7},
  title        = {Multiple insecticide resistance: an impediment to insecticide-based malaria vector control program},
  url          = {http://dx.doi.org/10.1371/journal.pone.0016066},
  volume       = {6},
  year         = {2011},
}

Chicago
Gebre, Delenasaw Yewhalaw, Walter Steurbaut, Pieter Spanoghe, Wim Van Bortel, Leen Denis, Dejene A Tessema, Yehenew Getachew Kifle, Marc Coosemans, Luc Duchateau, and Niko Speybroeck. 2011. “Multiple Insecticide Resistance: An Impediment to Insecticide-based Malaria Vector Control Program.” Plos One 6 (1).
APA
Gebre, D. Y., Steurbaut, W., Spanoghe, P., Van Bortel, W., Denis, L., Tessema, D. A., Kifle, Y. G., et al. (2011). Multiple insecticide resistance: an impediment to insecticide-based malaria vector control program. PLOS ONE, 6(1).
Vancouver
1.
Gebre DY, Steurbaut W, Spanoghe P, Van Bortel W, Denis L, Tessema DA, et al. Multiple insecticide resistance: an impediment to insecticide-based malaria vector control program. PLOS ONE. 2011;6(1).
MLA
Gebre, Delenasaw Yewhalaw, Walter Steurbaut, Pieter Spanoghe, et al. “Multiple Insecticide Resistance: An Impediment to Insecticide-based Malaria Vector Control Program.” PLOS ONE 6.1 (2011): n. pag. Print.