Advanced search
1 file | 110.28 KB

Multiple insecticide resistance: an impediment to insecticide-based malaria vector control program

(2011) PLOS ONE. 6(1).
Author
Organization
Abstract
Background: Indoor Residual Spraying (IRS), insecticide-treated nets (ITNs) and long-lasting insecticidal nets (LLINs) are key components in malaria prevention and control strategy. However, the development of resistance by mosquitoes to insecticides recommended for IRS and/or ITNs/LLINs would affect insecticide-based malaria vector control. We assessed the susceptibility levels of Anopheles arabiensis to insecticides used in malaria control, characterized basic mechanisms underlying resistance, and evaluated the role of public health use of insecticides in resistance selection. Methodology/Principal findings: Susceptibility status of An. arabiensis was assessed using WHO bioassay tests to DDT, permethrin, deltamethrin, malathion and propoxur in Ethiopia from August to September 2009. Mosquito specimens were screened for knockdown resistance (kdr) and insensitive acetylcholinesterase (ace-1(R)) mutations using AS-PCR and PCR-RFLP, respectively. DDT residues level in soil from human dwellings and the surrounding environment were determined by Gas Chromatography with Electron Capture Detector. An. arabiensis was resistant to DDT, permethrin, deltamethrin and malathion, but susceptible to propoxur. The West African kdr allele was found in 280 specimens out of 284 with a frequency ranged from 95% to 100%. Ace-1(R) mutation was not detected in all specimens scored for the allele. Moreover, DDT residues were found in soil samples from human dwellings but not in the surrounding environment. Conclusion: The observed multiple-resistance coupled with the occurrence of high kdr frequency in populations of An. arabiensis could profoundly affect the malaria vector control programme in Ethiopia. This needs an urgent call for implementing rational resistance management strategies and integrated vector control intervention.
Keywords
WEST-AFRICA, KDR MUTATION, KNOCKDOWN RESISTANCE, ANOPHELES-GAMBIAE COMPLEX, CULEX-QUINQUEFASCIATUS, NATURAL-POPULATIONS, MOSQUITO VECTORS, BURKINA-FASO, ARABIENSIS, DDT

Downloads

  • Multiple Insecticide Resistance.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 110.28 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Gebre, Delenasaw Yewhalaw, Walter Steurbaut, Pieter Spanoghe, Wim Van Bortel, Leen Denis, Dejene A Tessema, Yehenew Getachew Kifle, Marc Coosemans, Luc Duchateau, and Niko Speybroeck. 2011. “Multiple Insecticide Resistance: An Impediment to Insecticide-based Malaria Vector Control Program.” Plos One 6 (1).
APA
Gebre, D. Y., Steurbaut, W., Spanoghe, P., Van Bortel, W., Denis, L., Tessema, D. A., Kifle, Y. G., et al. (2011). Multiple insecticide resistance: an impediment to insecticide-based malaria vector control program. PLOS ONE, 6(1).
Vancouver
1.
Gebre DY, Steurbaut W, Spanoghe P, Van Bortel W, Denis L, Tessema DA, et al. Multiple insecticide resistance: an impediment to insecticide-based malaria vector control program. PLOS ONE. 2011;6(1).
MLA
Gebre, Delenasaw Yewhalaw, Walter Steurbaut, Pieter Spanoghe, et al. “Multiple Insecticide Resistance: An Impediment to Insecticide-based Malaria Vector Control Program.” PLOS ONE 6.1 (2011): n. pag. Print.
@article{1235081,
  abstract     = {Background: Indoor Residual Spraying (IRS), insecticide-treated nets (ITNs) and long-lasting insecticidal nets (LLINs) are key components in malaria prevention and control strategy. However, the development of resistance by mosquitoes to insecticides recommended for IRS and/or ITNs/LLINs would affect insecticide-based malaria vector control. We assessed the susceptibility levels of Anopheles arabiensis to insecticides used in malaria control, characterized basic mechanisms underlying resistance, and evaluated the role of public health use of insecticides in resistance selection. Methodology/Principal findings: Susceptibility status of An. arabiensis was assessed using WHO bioassay tests to DDT, permethrin, deltamethrin, malathion and propoxur in Ethiopia from August to September 2009. Mosquito specimens were screened for knockdown resistance (kdr) and insensitive acetylcholinesterase (ace-1(R)) mutations using AS-PCR and PCR-RFLP, respectively. DDT residues level in soil from human dwellings and the surrounding environment were determined by Gas Chromatography with Electron Capture Detector. An. arabiensis was resistant to DDT, permethrin, deltamethrin and malathion, but susceptible to propoxur. The West African kdr allele was found in 280 specimens out of 284 with a frequency ranged from 95\% to 100\%. Ace-1(R) mutation was not detected in all specimens scored for the allele. Moreover, DDT residues were found in soil samples from human dwellings but not in the surrounding environment. Conclusion: The observed multiple-resistance coupled with the occurrence of high kdr frequency in populations of An. arabiensis could profoundly affect the malaria vector control programme in Ethiopia. This needs an urgent call for implementing rational resistance management strategies and integrated vector control intervention.},
  articleno    = {e16066},
  author       = {Gebre, Delenasaw Yewhalaw and Steurbaut, Walter and Spanoghe, Pieter and Van Bortel, Wim and Denis, Leen and Tessema, Dejene A and Kifle, Yehenew Getachew and Coosemans, Marc and Duchateau, Luc and Speybroeck, Niko},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {WEST-AFRICA,KDR MUTATION,KNOCKDOWN RESISTANCE,ANOPHELES-GAMBIAE COMPLEX,CULEX-QUINQUEFASCIATUS,NATURAL-POPULATIONS,MOSQUITO VECTORS,BURKINA-FASO,ARABIENSIS,DDT},
  language     = {eng},
  number       = {1},
  pages        = {7},
  title        = {Multiple insecticide resistance: an impediment to insecticide-based malaria vector control program},
  url          = {http://dx.doi.org/10.1371/journal.pone.0016066},
  volume       = {6},
  year         = {2011},
}

Altmetric
View in Altmetric
Web of Science
Times cited: