Ghent University Academic Bibliography

Advanced

Differential gene expression of the toll-like receptor-4 cascade and neutrophil function in early- and mid-lactating dairy cows

Mieke Stevens UGent, Luc Peelman UGent, Bart De Spiegeleer UGent, Adel Pezeshki UGent, Gerlinde Van de Walle UGent, Luc Duchateau UGent and Christian Burvenich UGent (2011) JOURNAL OF DAIRY SCIENCE. 94(3). p.1277-1288
abstract
During early lactation, neutrophils display several reduced immune functions. Particularly, a delayed recruitment of neutrophils into the infected udder seems to be one of the underlying events involved in the severity of postpartum Escherichia coli intramammary infections. The purpose of this study was to analyze the effect of in vitro chemotaxis and diapedesis on the expression of toll-like receptor-4 (TLR4)-related genes in bovine blood neutrophils isolated from 10 early-lactating (EL) and 10 mid-lactating (ML) cows. Functional characterization of the neutrophil population was performed by measuring phagocytosis and production of reactive oxygen species (chemiluminescence). Messenger RNA was extracted from neutrophils, and the expression of TLR4 and associated genes in EL and ML cows was analyzed by reverse-transcription quantitative PCR. To study the effect of chemotaxis and diapedesis on the expression of genes of the TLR4 cascade, neutrophils were stimulated to (trans) migrate in response to C5a using in vitro models. Our salient findings were that both neutrophil migration in vitro and lactation stage induced significant changes in the expression of several genes of the TLR4 signaling cascade. Before migration, expression of TRAF6, ATF3, RELA, IL8, and C5aR were lower in EL than in ML cows. Diapedesis and chemotaxis induced an increase in expression of TLR4, ATF3, and IL8 in both EL and ML cows. Diapedesis resulted in a downregulation of Syk, a TLR4-associated gene, in ML cows. This study shows that the perturbations in neutrophil functions during EL are accompanied by modulation of TLR4 pathway genes. These data can contribute to the understanding of the mechanisms explaining the relationship between stage of lactation and risk of severe E. coli mastitis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
BOVINE POLYMORPHONUCLEAR LEUKOCYTES, ESCHERICHIA-COLI MASTITIS, TYROSINE KINASE, PERIPARTURIENT PERIOD, BIOLOGICAL FUNCTIONS, SIGNAL-TRANSDUCTION, IMMUNE-RESPONSES, SYK, TOLL-LIKE-RECEPTOR-4, CHEMILUMINESCENCE, diapedesis, chemotaxis, toll-like receptor-4 (TLR4), neutrophil
journal title
JOURNAL OF DAIRY SCIENCE
J. Dairy Sci.
volume
94
issue
3
pages
1277 - 1288
Web of Science type
Article
Web of Science id
000287473500021
JCR category
AGRICULTURE, DAIRY & ANIMAL SCIENCE
JCR impact factor
2.564 (2011)
JCR rank
2/54 (2011)
JCR quartile
1 (2011)
ISSN
0022-0302
DOI
10.3168/jds.2010-3563
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1235072
handle
http://hdl.handle.net/1854/LU-1235072
date created
2011-05-24 21:27:10
date last changed
2011-06-07 11:19:55
@article{1235072,
  abstract     = {During early lactation, neutrophils display several reduced immune functions. Particularly, a delayed recruitment of neutrophils into the infected udder seems to be one of the underlying events involved in the severity of postpartum Escherichia coli intramammary infections. The purpose of this study was to analyze the effect of in vitro chemotaxis and diapedesis on the expression of toll-like receptor-4 (TLR4)-related genes in bovine blood neutrophils isolated from 10 early-lactating (EL) and 10 mid-lactating (ML) cows. Functional characterization of the neutrophil population was performed by measuring phagocytosis and production of reactive oxygen species (chemiluminescence). Messenger RNA was extracted from neutrophils, and the expression of TLR4 and associated genes in EL and ML cows was analyzed by reverse-transcription quantitative PCR. To study the effect of chemotaxis and diapedesis on the expression of genes of the TLR4 cascade, neutrophils were stimulated to (trans) migrate in response to C5a using in vitro models. Our salient findings were that both neutrophil migration in vitro and lactation stage induced significant changes in the expression of several genes of the TLR4 signaling cascade. Before migration, expression of TRAF6, ATF3, RELA, IL8, and C5aR were lower in EL than in ML cows. Diapedesis and chemotaxis induced an increase in expression of TLR4, ATF3, and IL8 in both EL and ML cows. Diapedesis resulted in a downregulation of Syk, a TLR4-associated gene, in ML cows. This study shows that the perturbations in neutrophil functions during EL are accompanied by modulation of TLR4 pathway genes. These data can contribute to the understanding of the mechanisms explaining the relationship between stage of lactation and risk of severe E. coli mastitis.},
  author       = {Stevens, Mieke and Peelman, Luc and De Spiegeleer, Bart and Pezeshki, Adel and Van de Walle, Gerlinde and Duchateau, Luc and Burvenich, Christian},
  issn         = {0022-0302},
  journal      = {JOURNAL OF DAIRY SCIENCE},
  keyword      = {BOVINE POLYMORPHONUCLEAR LEUKOCYTES,ESCHERICHIA-COLI MASTITIS,TYROSINE KINASE,PERIPARTURIENT PERIOD,BIOLOGICAL FUNCTIONS,SIGNAL-TRANSDUCTION,IMMUNE-RESPONSES,SYK,TOLL-LIKE-RECEPTOR-4,CHEMILUMINESCENCE,diapedesis,chemotaxis,toll-like receptor-4 (TLR4),neutrophil},
  language     = {eng},
  number       = {3},
  pages        = {1277--1288},
  title        = {Differential gene expression of the toll-like receptor-4 cascade and neutrophil function in early- and mid-lactating dairy cows},
  url          = {http://dx.doi.org/10.3168/jds.2010-3563},
  volume       = {94},
  year         = {2011},
}

Chicago
Stevens, Mieke, Luc Peelman, Bart De Spiegeleer, Adel Pezeshki, Gerlinde Van de Walle, Luc Duchateau, and Christian Burvenich. 2011. “Differential Gene Expression of the Toll-like Receptor-4 Cascade and Neutrophil Function in Early- and Mid-lactating Dairy Cows.” Journal of Dairy Science 94 (3): 1277–1288.
APA
Stevens, Mieke, Peelman, L., De Spiegeleer, B., Pezeshki, A., Van de Walle, G., Duchateau, L., & Burvenich, C. (2011). Differential gene expression of the toll-like receptor-4 cascade and neutrophil function in early- and mid-lactating dairy cows. JOURNAL OF DAIRY SCIENCE, 94(3), 1277–1288.
Vancouver
1.
Stevens M, Peelman L, De Spiegeleer B, Pezeshki A, Van de Walle G, Duchateau L, et al. Differential gene expression of the toll-like receptor-4 cascade and neutrophil function in early- and mid-lactating dairy cows. JOURNAL OF DAIRY SCIENCE. 2011;94(3):1277–88.
MLA
Stevens, Mieke, Luc Peelman, Bart De Spiegeleer, et al. “Differential Gene Expression of the Toll-like Receptor-4 Cascade and Neutrophil Function in Early- and Mid-lactating Dairy Cows.” JOURNAL OF DAIRY SCIENCE 94.3 (2011): 1277–1288. Print.