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Cigarette smoking alters epithelial apoptosis and immune composition in murine GALT

STEPHANIE VERSCHUERE, Ken Bracke UGent, Tine Demoor, Maud Plantinga, Phebe Verbrugghe, Liesbeth Ferdinande UGent, Bart Lambrecht UGent, Guy Brusselle UGent and Claude Cuvelier (2011) LABORATORY INVESTIGATION. 91(7). p.1056-1067
abstract
Smokers have a twofold increased risk to develop Crohn's disease (CD). However, little is known about the mechanisms through which smoking affects CD pathogenesis. Especially Crohn's ileitis is negatively influenced by smoking. Interestingly, the ileum and, more in particular, the Peyer's patches in the terminal ileum are also the sites where the first CD lesions are found. Several chemokines are implicated in the pathogenesis, among which is the CCL20-CCR6 pathway. Here, we studied the gut-associated lymphoid tissue in C57BL/6 wild-type mice and in CCR6-deficient mice after exposure to air or cigarette smoke for 24 weeks. Apoptotic index of the follicle-associated epithelium overlying the Peyer's patches was evaluated. We found that chronic smoke exposure induced apoptosis in the follicle-associated epithelium. Furthermore, immune cell numbers and differentiation along with chemokine expression were determined in Peyer's patches. Important changes in immune cell composition were observed: total dendritic cells, CD4+ T cells (including regulatory T cells) and CD8+ T cells increased significantly after smoke exposure. The CD11b+ dendritic cell subset almost doubled. Interestingly, these changes were accompanied by an upregulated mRNA expression of the chemokines CCL9 and CCL20. However, no differences in the increase of dendritic cells were observed between wild-type and CCR6-deficient mice. Our results show that cigarette smoke exposure increases apoptosis in the follicle-associated epithelium and is associated with immune cell accumulation in Peyer's patches.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Crohn’s disease, dendritic cell, apoptosis, Peyer’s patch, cigarette smoke, intestinal epithelium, INFLAMMATORY-BOWEL-DISEASE, INDUCED PULMONARY INFLAMMATION, PATCH DENDRITIC CELLS, CROHNS-DISEASE, EXPERIMENTAL COLITIS, PEYERS-PATCHES, INTESTINAL INFLAMMATION, MICE, EXPRESSION, CHEMOKINE
journal title
LABORATORY INVESTIGATION
Lab. Invest.
volume
91
issue
7
pages
1056 - 1067
Web of Science type
Article
Web of Science id
000292189100008
JCR category
PATHOLOGY
JCR impact factor
3.641 (2011)
JCR rank
14/78 (2011)
JCR quartile
1 (2011)
ISSN
0023-6837
DOI
10.1038/labinvest.2011.74
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1234760
handle
http://hdl.handle.net/1854/LU-1234760
date created
2011-05-24 17:40:50
date last changed
2016-12-19 15:42:48
@article{1234760,
  abstract     = {Smokers have a twofold increased risk to develop Crohn's disease (CD). However, little is known about the mechanisms through which smoking affects CD pathogenesis. Especially Crohn's ileitis is negatively influenced by smoking. Interestingly, the ileum and, more in particular, the Peyer's patches in the terminal ileum are also the sites where the first CD lesions are found. Several chemokines are implicated in the pathogenesis, among which is the CCL20-CCR6 pathway. Here, we studied the gut-associated lymphoid tissue in C57BL/6 wild-type mice and in CCR6-deficient mice after exposure to air or cigarette smoke for 24 weeks. Apoptotic index of the follicle-associated epithelium overlying the Peyer's patches was evaluated. We found that chronic smoke exposure induced apoptosis in the follicle-associated epithelium. Furthermore, immune cell numbers and differentiation along with chemokine expression were determined in Peyer's patches. Important changes in immune cell composition were observed: total dendritic cells, CD4+ T cells (including regulatory T cells) and CD8+ T cells increased significantly after smoke exposure. The CD11b+ dendritic cell subset almost doubled. Interestingly, these changes were accompanied by an upregulated mRNA expression of the chemokines CCL9 and CCL20. However, no differences in the increase of dendritic cells were observed between wild-type and CCR6-deficient mice. Our results show that cigarette smoke exposure increases apoptosis in the follicle-associated epithelium and is associated with immune cell accumulation in Peyer's patches.},
  author       = {VERSCHUERE, STEPHANIE and Bracke, Ken and Demoor, Tine and Plantinga, Maud and Verbrugghe, Phebe  and Ferdinande, Liesbeth and Lambrecht, Bart and Brusselle, Guy and Cuvelier, Claude},
  issn         = {0023-6837},
  journal      = {LABORATORY INVESTIGATION},
  keyword      = {Crohn{\textquoteright}s disease,dendritic cell,apoptosis,Peyer{\textquoteright}s patch,cigarette smoke,intestinal epithelium,INFLAMMATORY-BOWEL-DISEASE,INDUCED PULMONARY INFLAMMATION,PATCH DENDRITIC CELLS,CROHNS-DISEASE,EXPERIMENTAL COLITIS,PEYERS-PATCHES,INTESTINAL INFLAMMATION,MICE,EXPRESSION,CHEMOKINE},
  language     = {eng},
  number       = {7},
  pages        = {1056--1067},
  title        = {Cigarette smoking alters epithelial apoptosis and immune composition in murine GALT},
  url          = {http://dx.doi.org/10.1038/labinvest.2011.74},
  volume       = {91},
  year         = {2011},
}

Chicago
VERSCHUERE, STEPHANIE, Ken Bracke, Tine Demoor, Maud Plantinga, Phebe Verbrugghe, Liesbeth Ferdinande, Bart Lambrecht, Guy Brusselle, and Claude Cuvelier. 2011. “Cigarette Smoking Alters Epithelial Apoptosis and Immune Composition in Murine GALT.” Laboratory Investigation 91 (7): 1056–1067.
APA
VERSCHUERE, S., Bracke, K., Demoor, T., Plantinga, M., Verbrugghe, P., Ferdinande, L., Lambrecht, B., et al. (2011). Cigarette smoking alters epithelial apoptosis and immune composition in murine GALT. LABORATORY INVESTIGATION, 91(7), 1056–1067.
Vancouver
1.
VERSCHUERE S, Bracke K, Demoor T, Plantinga M, Verbrugghe P, Ferdinande L, et al. Cigarette smoking alters epithelial apoptosis and immune composition in murine GALT. LABORATORY INVESTIGATION. 2011;91(7):1056–67.
MLA
VERSCHUERE, STEPHANIE, Ken Bracke, Tine Demoor, et al. “Cigarette Smoking Alters Epithelial Apoptosis and Immune Composition in Murine GALT.” LABORATORY INVESTIGATION 91.7 (2011): 1056–1067. Print.