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Cigarette smoking alters epithelial apoptosis and immune composition in murine GALT

STEPHANIE VERSCHUERE (UGent) , Ken Bracke (UGent) , Tine Demoor (UGent) , Maud Plantinga (UGent) , Phebe Verbrugghe, Liesbeth Ferdinande (UGent) , Bart Lambrecht (UGent) , Guy Brusselle (UGent) and Claude Cuvelier (UGent)
(2011) LABORATORY INVESTIGATION. 91(7). p.1056-1067
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Abstract
Smokers have a twofold increased risk to develop Crohn's disease (CD). However, little is known about the mechanisms through which smoking affects CD pathogenesis. Especially Crohn's ileitis is negatively influenced by smoking. Interestingly, the ileum and, more in particular, the Peyer's patches in the terminal ileum are also the sites where the first CD lesions are found. Several chemokines are implicated in the pathogenesis, among which is the CCL20-CCR6 pathway. Here, we studied the gut-associated lymphoid tissue in C57BL/6 wild-type mice and in CCR6-deficient mice after exposure to air or cigarette smoke for 24 weeks. Apoptotic index of the follicle-associated epithelium overlying the Peyer's patches was evaluated. We found that chronic smoke exposure induced apoptosis in the follicle-associated epithelium. Furthermore, immune cell numbers and differentiation along with chemokine expression were determined in Peyer's patches. Important changes in immune cell composition were observed: total dendritic cells, CD4+ T cells (including regulatory T cells) and CD8+ T cells increased significantly after smoke exposure. The CD11b+ dendritic cell subset almost doubled. Interestingly, these changes were accompanied by an upregulated mRNA expression of the chemokines CCL9 and CCL20. However, no differences in the increase of dendritic cells were observed between wild-type and CCR6-deficient mice. Our results show that cigarette smoke exposure increases apoptosis in the follicle-associated epithelium and is associated with immune cell accumulation in Peyer's patches.
Keywords
Crohn’s disease, dendritic cell, apoptosis, Peyer’s patch, cigarette smoke, intestinal epithelium, INFLAMMATORY-BOWEL-DISEASE, INDUCED PULMONARY INFLAMMATION, PATCH DENDRITIC CELLS, CROHNS-DISEASE, EXPERIMENTAL COLITIS, PEYERS-PATCHES, INTESTINAL INFLAMMATION, MICE, EXPRESSION, CHEMOKINE

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Chicago
VERSCHUERE, STEPHANIE, Ken Bracke, Tine Demoor, Maud Plantinga, Phebe Verbrugghe, Liesbeth Ferdinande, Bart Lambrecht, Guy Brusselle, and Claude Cuvelier. 2011. “Cigarette Smoking Alters Epithelial Apoptosis and Immune Composition in Murine GALT.” Laboratory Investigation 91 (7): 1056–1067.
APA
VERSCHUERE, S., Bracke, K., Demoor, T., Plantinga, M., Verbrugghe, P., Ferdinande, L., Lambrecht, B., et al. (2011). Cigarette smoking alters epithelial apoptosis and immune composition in murine GALT. LABORATORY INVESTIGATION, 91(7), 1056–1067.
Vancouver
1.
VERSCHUERE S, Bracke K, Demoor T, Plantinga M, Verbrugghe P, Ferdinande L, et al. Cigarette smoking alters epithelial apoptosis and immune composition in murine GALT. LABORATORY INVESTIGATION. 2011;91(7):1056–67.
MLA
VERSCHUERE, STEPHANIE, Ken Bracke, Tine Demoor, et al. “Cigarette Smoking Alters Epithelial Apoptosis and Immune Composition in Murine GALT.” LABORATORY INVESTIGATION 91.7 (2011): 1056–1067. Print.
@article{1234760,
  abstract     = {Smokers have a twofold increased risk to develop Crohn's disease (CD). However, little is known about the mechanisms through which smoking affects CD pathogenesis. Especially Crohn's ileitis is negatively influenced by smoking. Interestingly, the ileum and, more in particular, the Peyer's patches in the terminal ileum are also the sites where the first CD lesions are found. Several chemokines are implicated in the pathogenesis, among which is the CCL20-CCR6 pathway. Here, we studied the gut-associated lymphoid tissue in C57BL/6 wild-type mice and in CCR6-deficient mice after exposure to air or cigarette smoke for 24 weeks. Apoptotic index of the follicle-associated epithelium overlying the Peyer's patches was evaluated. We found that chronic smoke exposure induced apoptosis in the follicle-associated epithelium. Furthermore, immune cell numbers and differentiation along with chemokine expression were determined in Peyer's patches. Important changes in immune cell composition were observed: total dendritic cells, CD4+ T cells (including regulatory T cells) and CD8+ T cells increased significantly after smoke exposure. The CD11b+ dendritic cell subset almost doubled. Interestingly, these changes were accompanied by an upregulated mRNA expression of the chemokines CCL9 and CCL20. However, no differences in the increase of dendritic cells were observed between wild-type and CCR6-deficient mice. Our results show that cigarette smoke exposure increases apoptosis in the follicle-associated epithelium and is associated with immune cell accumulation in Peyer's patches.},
  author       = {VERSCHUERE, STEPHANIE and Bracke, Ken and Demoor, Tine and Plantinga, Maud and Verbrugghe, Phebe  and Ferdinande, Liesbeth and Lambrecht, Bart and Brusselle, Guy and Cuvelier, Claude},
  issn         = {0023-6837},
  journal      = {LABORATORY INVESTIGATION},
  keyword      = {Crohn{\textquoteright}s disease,dendritic cell,apoptosis,Peyer{\textquoteright}s patch,cigarette smoke,intestinal epithelium,INFLAMMATORY-BOWEL-DISEASE,INDUCED PULMONARY INFLAMMATION,PATCH DENDRITIC CELLS,CROHNS-DISEASE,EXPERIMENTAL COLITIS,PEYERS-PATCHES,INTESTINAL INFLAMMATION,MICE,EXPRESSION,CHEMOKINE},
  language     = {eng},
  number       = {7},
  pages        = {1056--1067},
  title        = {Cigarette smoking alters epithelial apoptosis and immune composition in murine GALT},
  url          = {http://dx.doi.org/10.1038/labinvest.2011.74},
  volume       = {91},
  year         = {2011},
}

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