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Complex III staining in blue native polyacrylamide gels

Joél Smet UGent, Boel De Paepe UGent, Sara Seneca, Willy Lissens, Heike Kotarsky, Linda De Meirleir, Vineta Fellman and Rudy Van Coster UGent (2011) JOURNAL OF INHERITED METABOLIC DISEASE. 34(3). p.741-747
abstract
For more than a decade now blue native polyacrylamide gel electrophoresis (BN-PAGE) has been used for the study of the oxidative phosphorylation (OXPHOS) complexes. Catalytic activities of complexes I, II, IV and V can be assessed, after separation by gel electroforesis, by incubation of the BN-PAGE gel in specific staining solutions. However, until now, a reliable staining method for testing ubiquinol cytochrome c oxidoreductase (complex III) activity by BN-PAGE gel techniques was not available. In addition, spectrophotometric methods currently in use for detection of complex III deficiency in patients are not very sensitive. Here, we describe a newly developed diagnostic method for visualization of complex III activity by direct in-gel evaluation of ubiquinol cytochrome oxidoreductase activity. We validated the method by reporting the results in six patients with previously characterised complex III defects.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
OXIDATIVE-PHOSPHORYLATION, LETHAL METABOLIC-DISORDER, GRACILE SYNDROME, IRON OVERLOAD, BCS1L GENE, DEFICIENCY, MUTATION, ELECTROPHORESIS, ASSAYS
journal title
JOURNAL OF INHERITED METABOLIC DISEASE
J. Inherit. Metab. Dis.
volume
34
issue
3
pages
741 - 747
Web of Science type
Article
Web of Science id
000291360100022
JCR category
GENETICS & HEREDITY
JCR impact factor
3.577 (2011)
JCR rank
46/155 (2011)
JCR quartile
2 (2011)
ISSN
0141-8955
DOI
10.1007/s10545-011-9315-7
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1233553
handle
http://hdl.handle.net/1854/LU-1233553
date created
2011-05-24 13:49:39
date last changed
2016-12-19 15:42:48
@article{1233553,
  abstract     = {For more than a decade now blue native polyacrylamide gel electrophoresis (BN-PAGE) has been used for the study of the oxidative phosphorylation (OXPHOS) complexes. Catalytic activities of complexes I, II, IV and V can be assessed, after separation by gel electroforesis, by incubation of the BN-PAGE gel in specific staining solutions. However, until now, a reliable staining method for testing ubiquinol cytochrome c oxidoreductase (complex III) activity by BN-PAGE gel techniques was not available. In addition, spectrophotometric methods currently in use for detection of complex III deficiency in patients are not very sensitive. Here, we describe a newly developed diagnostic method for visualization of complex III activity by direct in-gel evaluation of ubiquinol cytochrome oxidoreductase activity. We validated the method by reporting the results in six patients with previously characterised complex III defects.},
  author       = {Smet, Jo{\'e}l and De Paepe, Boel and Seneca, Sara and Lissens, Willy and Kotarsky, Heike and De Meirleir, Linda and Fellman, Vineta and Van Coster, Rudy},
  issn         = {0141-8955},
  journal      = {JOURNAL OF INHERITED METABOLIC DISEASE},
  keyword      = {OXIDATIVE-PHOSPHORYLATION,LETHAL METABOLIC-DISORDER,GRACILE SYNDROME,IRON OVERLOAD,BCS1L GENE,DEFICIENCY,MUTATION,ELECTROPHORESIS,ASSAYS},
  language     = {eng},
  number       = {3},
  pages        = {741--747},
  title        = {Complex III staining in blue native polyacrylamide gels},
  url          = {http://dx.doi.org/10.1007/s10545-011-9315-7},
  volume       = {34},
  year         = {2011},
}

Chicago
Smet, Joél, Boel De Paepe, Sara Seneca, Willy Lissens, Heike Kotarsky, Linda De Meirleir, Vineta Fellman, and Rudy Van Coster. 2011. “Complex III Staining in Blue Native Polyacrylamide Gels.” Journal of Inherited Metabolic Disease 34 (3): 741–747.
APA
Smet, Joél, De Paepe, B., Seneca, S., Lissens, W., Kotarsky, H., De Meirleir, L., Fellman, V., et al. (2011). Complex III staining in blue native polyacrylamide gels. JOURNAL OF INHERITED METABOLIC DISEASE, 34(3), 741–747.
Vancouver
1.
Smet J, De Paepe B, Seneca S, Lissens W, Kotarsky H, De Meirleir L, et al. Complex III staining in blue native polyacrylamide gels. JOURNAL OF INHERITED METABOLIC DISEASE. 2011;34(3):741–7.
MLA
Smet, Joél, Boel De Paepe, Sara Seneca, et al. “Complex III Staining in Blue Native Polyacrylamide Gels.” JOURNAL OF INHERITED METABOLIC DISEASE 34.3 (2011): 741–747. Print.