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Specific anti-nuclear antibodies in systemic sclerosis patients with and without skin involvement: an extended methodological approach

Jens Van Praet UGent, Katleen Van Steendam UGent, Vanessa Smith UGent, Georges De Bruyne UGent, Tsuneyo Mimori, Carolien Bonroy UGent, Dirk Elewaut UGent, Dieter Deforce UGent and Filip De Keyser UGent (2011) RHEUMATOLOGY. 50(7). p.1302-1309
abstract
Methods. Serum samples from 145 consecutive monocentric SSc patients fulfilling LeRoy and Medgser's criteria for early SSc were studied. ANAs were detected by IIF on HEp-2000 cells and identified by western blotting, protein radio-immunoprecipitation, RNA immunoprecipitation and line immunoassay (LIA). SSc organ involvement was assessed according to a modification of Medsger's disease severity scale. Results. At least one specific ANA reactivity was present in 88% of the patients. The standard algorithm (IIF and LIA) found at least one specific ANA in 74% of the patients. The main reactivities missed by this algorithm were anti-RNA polymerase III, anti-PM/Scl and anti-Th/To. Eighty-three percent of the patients with limited SSc had at least one ANA. ACAs and anti-Th/To antibodies were significantly associated with limited SSc, whereas anti-topoisomerase I and anti-RNA polymerase III were observed less frequently. SSc organ system involvement was present in 63% of the patients with limited SSc, most of whom had lung involvement. Conclusions. Standard algorithms for ANA identification lack sensitivity for the detection of SSc-associated ANA and should be supplemented with additional assays, especially in a clinical environment that has particular interest in SSc. The spectrum of SSc-associated ANA differs according to the presence or absence of skin involvement.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
MULTICENTER VALIDATION, CONNECTIVE-TISSUE DISORDERS, MICROVASCULAR DAMAGE, AUTOANTIBODIES, CLASSIFICATION, PROGNOSIS, CRITERIA, SUBSETS, COHORT, NAILFOLD VIDEOCAPILLAROSCOPY ASSESSMENT, Anti-RNA polymerase III, Anti-centromere, Anti-topoisomerase I, Western blotting, Immunoprecipitation, Systemic sclerosis, Anti-nuclear antibodies, Disease severity scale
journal title
RHEUMATOLOGY
Rheumatology
volume
50
issue
7
pages
1302 - 1309
Web of Science type
Article
Web of Science id
000291746100020
JCR category
RHEUMATOLOGY
JCR impact factor
4.058 (2011)
JCR rank
9/29 (2011)
JCR quartile
2 (2011)
ISSN
1462-0324
DOI
10.1093/rheumatology/keq446
project
Ghent researchers on unfolded proteins in inflammatory disease (GROUP-ID)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1232951
handle
http://hdl.handle.net/1854/LU-1232951
date created
2011-05-24 11:45:43
date last changed
2013-02-27 09:09:21
@article{1232951,
  abstract     = {Methods. Serum samples from 145 consecutive monocentric SSc patients fulfilling LeRoy and Medgser's criteria for early SSc were studied. ANAs were detected by IIF on HEp-2000 cells and identified by western blotting, protein radio-immunoprecipitation, RNA immunoprecipitation and line immunoassay (LIA). SSc organ involvement was assessed according to a modification of Medsger's disease severity scale. 
Results. At least one specific ANA reactivity was present in 88\% of the patients. The standard algorithm (IIF and LIA) found at least one specific ANA in 74\% of the patients. The main reactivities missed by this algorithm were anti-RNA polymerase III, anti-PM/Scl and anti-Th/To. Eighty-three percent of the patients with limited SSc had at least one ANA. ACAs and anti-Th/To antibodies were significantly associated with limited SSc, whereas anti-topoisomerase I and anti-RNA polymerase III were observed less frequently. SSc organ system involvement was present in 63\% of the patients with limited SSc, most of whom had lung involvement. 
Conclusions. Standard algorithms for ANA identification lack sensitivity for the detection of SSc-associated ANA and should be supplemented with additional assays, especially in a clinical environment that has particular interest in SSc. The spectrum of SSc-associated ANA differs according to the presence or absence of skin involvement.},
  author       = {Van Praet, Jens and Van Steendam, Katleen and Smith, Vanessa and De Bruyne, Georges and Mimori, Tsuneyo and Bonroy, Carolien and Elewaut, Dirk and Deforce, Dieter and De Keyser, Filip},
  issn         = {1462-0324},
  journal      = {RHEUMATOLOGY},
  keyword      = {MULTICENTER VALIDATION,CONNECTIVE-TISSUE DISORDERS,MICROVASCULAR DAMAGE,AUTOANTIBODIES,CLASSIFICATION,PROGNOSIS,CRITERIA,SUBSETS,COHORT,NAILFOLD VIDEOCAPILLAROSCOPY ASSESSMENT,Anti-RNA polymerase III,Anti-centromere,Anti-topoisomerase I,Western blotting,Immunoprecipitation,Systemic sclerosis,Anti-nuclear antibodies,Disease severity scale},
  language     = {eng},
  number       = {7},
  pages        = {1302--1309},
  title        = {Specific anti-nuclear antibodies in systemic sclerosis patients with and without skin involvement: an extended methodological approach},
  url          = {http://dx.doi.org/10.1093/rheumatology/keq446},
  volume       = {50},
  year         = {2011},
}

Chicago
VAN PRAET, JENS, Katleen Van Steendam, VANESSA SMITH, Georges De Bruyne, Tsuneyo Mimori, Carolien Bonroy, Dirk Elewaut, Dieter Deforce, and Filip De Keyser. 2011. “Specific Anti-nuclear Antibodies in Systemic Sclerosis Patients with and Without Skin Involvement: An Extended Methodological Approach.” Rheumatology 50 (7): 1302–1309.
APA
VAN PRAET, J., Van Steendam, K., SMITH, V., De Bruyne, G., Mimori, T., Bonroy, C., Elewaut, D., et al. (2011). Specific anti-nuclear antibodies in systemic sclerosis patients with and without skin involvement: an extended methodological approach. RHEUMATOLOGY, 50(7), 1302–1309.
Vancouver
1.
VAN PRAET J, Van Steendam K, SMITH V, De Bruyne G, Mimori T, Bonroy C, et al. Specific anti-nuclear antibodies in systemic sclerosis patients with and without skin involvement: an extended methodological approach. RHEUMATOLOGY. 2011;50(7):1302–9.
MLA
VAN PRAET, JENS, Katleen Van Steendam, VANESSA SMITH, et al. “Specific Anti-nuclear Antibodies in Systemic Sclerosis Patients with and Without Skin Involvement: An Extended Methodological Approach.” RHEUMATOLOGY 50.7 (2011): 1302–1309. Print.