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Intracellular nanoparticle coating stability determines nanoparticle diagnostics efficacy and cell functionality

(2010) SMALL. 6(19). p.2136-2145
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Abstract
Iron oxide nanoparticles (NPs) are frequently employed in biomedical research as magnetic resonance (MR) contrast agents where high intracellular levels are required to clearly depict signal alterations. To date, the toxicity and applicability of these particles have not been completely unraveled. Here, we show that endosomal localization of different iron oxide particles results in their degradation and in reduced MR contrast, the rate of which is governed mainly by the stability of the coating. The release of ferric iron generates reactive species, which greatly affect cell functionality. Lipid-coated NPs display the highest stability and furthermore exhibit intracellular clustering, which significantly enhances their MR properties and intracellular persistence. These findings are of considerable importance because, depending on the nature of the coating, particles can be rapidly degraded, thus completely annihilating their MR contrast to levels not detectable when compared to controls and greatly impeding cell functionality, thereby hindering their application in functional in vivo studies.
Keywords
CONTRAST AGENT, VIVO, STEM-CELLS, IN-VITRO, SUPERPARAMAGNETIC IRON-OXIDE, TRANSFERRIN RECEPTOR, MAGNETOLIPOSOMES, EXPRESSION, PARTICLES, TRACKING

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MLA
Soenen, Stefaan, Uwe Himmelreich, Nele Nuytten, et al. “Intracellular Nanoparticle Coating Stability Determines Nanoparticle Diagnostics Efficacy and Cell Functionality.” SMALL 6.19 (2010): 2136–2145. Print.
APA
Soenen, S., Himmelreich, U., Nuytten, N., Pisanic, T., Ferrari, A., & De Cuyper, M. (2010). Intracellular nanoparticle coating stability determines nanoparticle diagnostics efficacy and cell functionality. SMALL, 6(19), 2136–2145.
Chicago author-date
Soenen, Stefaan, Uwe Himmelreich, Nele Nuytten, Thomas Pisanic, Aldo Ferrari, and Marcel De Cuyper. 2010. “Intracellular Nanoparticle Coating Stability Determines Nanoparticle Diagnostics Efficacy and Cell Functionality.” Small 6 (19): 2136–2145.
Chicago author-date (all authors)
Soenen, Stefaan, Uwe Himmelreich, Nele Nuytten, Thomas Pisanic, Aldo Ferrari, and Marcel De Cuyper. 2010. “Intracellular Nanoparticle Coating Stability Determines Nanoparticle Diagnostics Efficacy and Cell Functionality.” Small 6 (19): 2136–2145.
Vancouver
1.
Soenen S, Himmelreich U, Nuytten N, Pisanic T, Ferrari A, De Cuyper M. Intracellular nanoparticle coating stability determines nanoparticle diagnostics efficacy and cell functionality. SMALL. 2010;6(19):2136–45.
IEEE
[1]
S. Soenen, U. Himmelreich, N. Nuytten, T. Pisanic, A. Ferrari, and M. De Cuyper, “Intracellular nanoparticle coating stability determines nanoparticle diagnostics efficacy and cell functionality,” SMALL, vol. 6, no. 19, pp. 2136–2145, 2010.
@article{1231640,
  abstract     = {Iron oxide nanoparticles (NPs) are frequently employed in biomedical research as magnetic resonance (MR) contrast agents where high intracellular levels are required to clearly depict signal alterations. To date, the toxicity and applicability of these particles have not been completely unraveled. Here, we show that endosomal localization of different iron oxide particles results in their degradation and in reduced MR contrast, the rate of which is governed mainly by the stability of the coating. The release of ferric iron generates reactive species, which greatly affect cell functionality. Lipid-coated NPs display the highest stability and furthermore exhibit intracellular clustering, which significantly enhances their MR properties and intracellular persistence. These findings are of considerable importance because, depending on the nature of the coating, particles can be rapidly degraded, thus completely annihilating their MR contrast to levels not detectable when compared to controls and greatly impeding cell functionality, thereby hindering their application in functional in vivo studies.},
  author       = {Soenen, Stefaan and Himmelreich, Uwe and Nuytten, Nele and Pisanic, Thomas and Ferrari, Aldo and De Cuyper, Marcel},
  issn         = {1613-6810},
  journal      = {SMALL},
  keywords     = {CONTRAST AGENT,VIVO,STEM-CELLS,IN-VITRO,SUPERPARAMAGNETIC IRON-OXIDE,TRANSFERRIN RECEPTOR,MAGNETOLIPOSOMES,EXPRESSION,PARTICLES,TRACKING},
  language     = {eng},
  number       = {19},
  pages        = {2136--2145},
  title        = {Intracellular nanoparticle coating stability determines nanoparticle diagnostics efficacy and cell functionality},
  url          = {http://dx.doi.org/10.1002/smll.201000763},
  volume       = {6},
  year         = {2010},
}

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