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High intracellular iron oxide nanoparticle concentrations affect cellular cytoskeleton and focal adhesion kinase-mediated signaling

Stefaan Soenen, Nele Nuytten, Simon De Meyer, Stefaan De Smedt UGent and Marcel De Cuyper (2010) SMALL. 6(7). p.832-842
abstract
Iron oxide nanoparticle internalization exerts detrimental effects on cell physiology for a variety of particles, hut little is known about the mechanism involved. The effects of high intracellular levels of four types of iron oxide particles (Resovist, Endorem, very small organic particles, and magneto-liposotnes (MLs)) on the viability and physiology of murine C17.2 neural progenitor cells and human blood outgrowth. endothelial cells are reported. The particles diminish cellular proliferation and affect the actin cytoskeleton and microtubule network architectures as well as focal adhesion formation and maturation. The extent of the effects correlates with the intracellular concentration (= iron mass) of the particles, with the biggest effects for Resovist and MLs at the highest concentration (1000 mu g Fe mL(-1)). Similarly, the expression of focal adhesion kinase (FAK) and the amount of activated kinase (pY397-FAK) are affected. The data suggest that high levels of perinuclear localized iron oxide nanoparticles diminish the efficiency of protein expression and sterically hinder the mature actin fibers, and could have detrimental effects on cell migration and differentiation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
cells, cytotoxicity, biomedical materials, ENDOCYTOSIS, CYTOTOXICITY, MAGNETOLIPOSOMES, FAK, PROLIFERATION, TOXICITY, MESENCHYMAL STEM-CELLS, nanoparticles, magnetic materials, CONTRAST AGENTS, IN-VITRO, MAGNETIC NANOPARTICLES
journal title
SMALL
Small
volume
6
issue
7
pages
832 - 842
Web of Science type
Article
Web of Science id
000277233000007
JCR category
MATERIALS SCIENCE, MULTIDISCIPLINARY
JCR impact factor
7.333 (2010)
JCR rank
11/219 (2010)
JCR quartile
1 (2010)
ISSN
1613-6810
DOI
10.1002/smll.200902084
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1231527
handle
http://hdl.handle.net/1854/LU-1231527
date created
2011-05-24 09:14:54
date last changed
2016-12-19 15:44:49
@article{1231527,
  abstract     = {Iron oxide nanoparticle internalization exerts detrimental effects on cell physiology for a variety of particles, hut little is known about the mechanism involved. The effects of high intracellular levels of four types of iron oxide particles (Resovist, Endorem, very small organic particles, and magneto-liposotnes (MLs)) on the viability and physiology of murine C17.2 neural progenitor cells and human blood outgrowth. endothelial cells are reported. The particles diminish cellular proliferation and affect the actin cytoskeleton and microtubule network architectures as well as focal adhesion formation and maturation. The extent of the effects correlates with the intracellular concentration (= iron mass) of the particles, with the biggest effects for Resovist and MLs at the highest concentration (1000 mu g Fe mL(-1)). Similarly, the expression of focal adhesion kinase (FAK) and the amount of activated kinase (pY397-FAK) are affected. The data suggest that high levels of perinuclear localized iron oxide nanoparticles diminish the efficiency of protein expression and sterically hinder the mature actin fibers, and could have detrimental effects on cell migration and differentiation.},
  author       = {Soenen, Stefaan and Nuytten, Nele and De Meyer, Simon and De Smedt, Stefaan and De Cuyper, Marcel},
  issn         = {1613-6810},
  journal      = {SMALL},
  keyword      = {cells,cytotoxicity,biomedical materials,ENDOCYTOSIS,CYTOTOXICITY,MAGNETOLIPOSOMES,FAK,PROLIFERATION,TOXICITY,MESENCHYMAL STEM-CELLS,nanoparticles,magnetic materials,CONTRAST AGENTS,IN-VITRO,MAGNETIC NANOPARTICLES},
  language     = {eng},
  number       = {7},
  pages        = {832--842},
  title        = {High intracellular iron oxide nanoparticle concentrations affect cellular cytoskeleton and focal adhesion kinase-mediated signaling},
  url          = {http://dx.doi.org/10.1002/smll.200902084},
  volume       = {6},
  year         = {2010},
}

Chicago
Soenen, Stefaan, Nele Nuytten, Simon De Meyer, Stefaan De Smedt, and Marcel De Cuyper. 2010. “High Intracellular Iron Oxide Nanoparticle Concentrations Affect Cellular Cytoskeleton and Focal Adhesion Kinase-mediated Signaling.” Small 6 (7): 832–842.
APA
Soenen, S., Nuytten, N., De Meyer, S., De Smedt, S., & De Cuyper, M. (2010). High intracellular iron oxide nanoparticle concentrations affect cellular cytoskeleton and focal adhesion kinase-mediated signaling. SMALL, 6(7), 832–842.
Vancouver
1.
Soenen S, Nuytten N, De Meyer S, De Smedt S, De Cuyper M. High intracellular iron oxide nanoparticle concentrations affect cellular cytoskeleton and focal adhesion kinase-mediated signaling. SMALL. 2010;6(7):832–42.
MLA
Soenen, Stefaan, Nele Nuytten, Simon De Meyer, et al. “High Intracellular Iron Oxide Nanoparticle Concentrations Affect Cellular Cytoskeleton and Focal Adhesion Kinase-mediated Signaling.” SMALL 6.7 (2010): 832–842. Print.