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The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniae and comparison with four other species specific PCR assays

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Abstract
Background: Streptococcus pneumoniae is one of the most frequently encountered pathogens in humans but its differentiation from closely related but less pathogenic streptococci remains a challenge. Methods: This report describes a newly-developed PCR assay (Spne-PCR), amplifying a 217 bp product of the 16S rRNA gene of S. pneumoniae, and its performance compared to other genotypic and phenotypic tests. Results: The new PCR assay designed in this study, proved to be specific at 57 degrees C for S. pneumoniae, not amplifying S. pseudopneumoniae or any other streptococcal strain or any strains from other upper airway pathogenic species. PCR assays (psaA, LytA, ply, spn9802-PCR) were previously described for the specific amplification of S. pneumoniae, but psaA-PCR was the only one found not to cross-react with S. pseudopneumoniae. Conclusion: Spne-PCR, developed for this study, and psaA-PCR were the only two assays which did not mis-identify S. pseudopneumoniae as S. pneumoniae. Four other PCR assays and the AccuProbe assay were unable to distinguish between these species.
Keywords
DIAGNOSIS, PORTUGAL, PNEUMOCOCCUS, EPIDEMIOLOGY, HYBRIDIZATION, DNA, POLYMERASE-CHAIN-REACTION, OPTOCHIN RESISTANCE, LYTA, PSAA

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MLA
El Aila, Nabil, et al. “The Development of a 16S RRNA Gene Based PCR for the Identification of Streptococcus Pneumoniae and Comparison with Four Other Species Specific PCR Assays.” BMC INFECTIOUS DISEASES, vol. 10, 2010, doi:10.1186/1471-2334-10-104.
APA
El Aila, N., Emler, S., Kaijalainen, T., De Baere, T., Saerens, B., Alkan, E., … Vaneechoutte, M. (2010). The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniae and comparison with four other species specific PCR assays. BMC INFECTIOUS DISEASES, 10. https://doi.org/10.1186/1471-2334-10-104
Chicago author-date
El Aila, Nabil, Stefan Emler, Tarja Kaijalainen, Thierry De Baere, Bart Saerens, Elife Alkan, Pieter Deschaght, Rita Verhelst, and Mario Vaneechoutte. 2010. “The Development of a 16S RRNA Gene Based PCR for the Identification of Streptococcus Pneumoniae and Comparison with Four Other Species Specific PCR Assays.” BMC INFECTIOUS DISEASES 10. https://doi.org/10.1186/1471-2334-10-104.
Chicago author-date (all authors)
El Aila, Nabil, Stefan Emler, Tarja Kaijalainen, Thierry De Baere, Bart Saerens, Elife Alkan, Pieter Deschaght, Rita Verhelst, and Mario Vaneechoutte. 2010. “The Development of a 16S RRNA Gene Based PCR for the Identification of Streptococcus Pneumoniae and Comparison with Four Other Species Specific PCR Assays.” BMC INFECTIOUS DISEASES 10. doi:10.1186/1471-2334-10-104.
Vancouver
1.
El Aila N, Emler S, Kaijalainen T, De Baere T, Saerens B, Alkan E, et al. The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniae and comparison with four other species specific PCR assays. BMC INFECTIOUS DISEASES. 2010;10.
IEEE
[1]
N. El Aila et al., “The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniae and comparison with four other species specific PCR assays,” BMC INFECTIOUS DISEASES, vol. 10, 2010.
@article{1231314,
  abstract     = {{Background: Streptococcus pneumoniae is one of the most frequently encountered pathogens in humans but its differentiation from closely related but less pathogenic streptococci remains a challenge.
Methods: This report describes a newly-developed PCR assay (Spne-PCR), amplifying a 217 bp product of the 16S rRNA gene of S. pneumoniae, and its performance compared to other genotypic and phenotypic tests.
Results: The new PCR assay designed in this study, proved to be specific at 57 degrees C for S. pneumoniae, not amplifying S. pseudopneumoniae or any other streptococcal strain or any strains from other upper airway pathogenic species. PCR assays (psaA, LytA, ply, spn9802-PCR) were previously described for the specific amplification of S. pneumoniae, but psaA-PCR was the only one found not to cross-react with S. pseudopneumoniae.
Conclusion: Spne-PCR, developed for this study, and psaA-PCR were the only two assays which did not mis-identify S. pseudopneumoniae as S. pneumoniae. Four other PCR assays and the AccuProbe assay were unable to distinguish between these species.}},
  articleno    = {{104}},
  author       = {{El Aila, Nabil and Emler, Stefan and Kaijalainen, Tarja and De Baere, Thierry and Saerens, Bart and Alkan, Elife and Deschaght, Pieter and Verhelst, Rita and Vaneechoutte, Mario}},
  issn         = {{1471-2334}},
  journal      = {{BMC INFECTIOUS DISEASES}},
  keywords     = {{DIAGNOSIS,PORTUGAL,PNEUMOCOCCUS,EPIDEMIOLOGY,HYBRIDIZATION,DNA,POLYMERASE-CHAIN-REACTION,OPTOCHIN RESISTANCE,LYTA,PSAA}},
  language     = {{eng}},
  pages        = {{8}},
  title        = {{The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniae and comparison with four other species specific PCR assays}},
  url          = {{http://dx.doi.org/10.1186/1471-2334-10-104}},
  volume       = {{10}},
  year         = {{2010}},
}

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