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The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniae and comparison with four other species specific PCR assays

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Abstract
Background: Streptococcus pneumoniae is one of the most frequently encountered pathogens in humans but its differentiation from closely related but less pathogenic streptococci remains a challenge. Methods: This report describes a newly-developed PCR assay (Spne-PCR), amplifying a 217 bp product of the 16S rRNA gene of S. pneumoniae, and its performance compared to other genotypic and phenotypic tests. Results: The new PCR assay designed in this study, proved to be specific at 57 degrees C for S. pneumoniae, not amplifying S. pseudopneumoniae or any other streptococcal strain or any strains from other upper airway pathogenic species. PCR assays (psaA, LytA, ply, spn9802-PCR) were previously described for the specific amplification of S. pneumoniae, but psaA-PCR was the only one found not to cross-react with S. pseudopneumoniae. Conclusion: Spne-PCR, developed for this study, and psaA-PCR were the only two assays which did not mis-identify S. pseudopneumoniae as S. pneumoniae. Four other PCR assays and the AccuProbe assay were unable to distinguish between these species.
Keywords
DIAGNOSIS, PORTUGAL, PNEUMOCOCCUS, EPIDEMIOLOGY, HYBRIDIZATION, DNA, POLYMERASE-CHAIN-REACTION, OPTOCHIN RESISTANCE, LYTA, PSAA

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Chicago
El Aila, Nabil, Stefan Emler, Tarja Kaijalainen, Thierry De Baere, Bart Saerens, Elife Alkan, Pieter Deschaght, Rita Verhelst, and Mario Vaneechoutte. 2010. “The Development of a 16S rRNA Gene Based PCR for the Identification of Streptococcus Pneumoniae and Comparison with Four Other Species Specific PCR Assays.” Bmc Infectious Diseases 10.
APA
El Aila, N., Emler, S., Kaijalainen, T., De Baere, T., Saerens, B., Alkan, E., Deschaght, P., et al. (2010). The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniae and comparison with four other species specific PCR assays. BMC INFECTIOUS DISEASES, 10.
Vancouver
1.
El Aila N, Emler S, Kaijalainen T, De Baere T, Saerens B, Alkan E, et al. The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniae and comparison with four other species specific PCR assays. BMC INFECTIOUS DISEASES. 2010;10.
MLA
El Aila, Nabil, Stefan Emler, Tarja Kaijalainen, et al. “The Development of a 16S rRNA Gene Based PCR for the Identification of Streptococcus Pneumoniae and Comparison with Four Other Species Specific PCR Assays.” BMC INFECTIOUS DISEASES 10 (2010): n. pag. Print.
@article{1231314,
  abstract     = {Background: Streptococcus pneumoniae is one of the most frequently encountered pathogens in humans but its differentiation from closely related but less pathogenic streptococci remains a challenge.
Methods: This report describes a newly-developed PCR assay (Spne-PCR), amplifying a 217 bp product of the 16S rRNA gene of S. pneumoniae, and its performance compared to other genotypic and phenotypic tests.
Results: The new PCR assay designed in this study, proved to be specific at 57 degrees C for S. pneumoniae, not amplifying S. pseudopneumoniae or any other streptococcal strain or any strains from other upper airway pathogenic species. PCR assays (psaA, LytA, ply, spn9802-PCR) were previously described for the specific amplification of S. pneumoniae, but psaA-PCR was the only one found not to cross-react with S. pseudopneumoniae.
Conclusion: Spne-PCR, developed for this study, and psaA-PCR were the only two assays which did not mis-identify S. pseudopneumoniae as S. pneumoniae. Four other PCR assays and the AccuProbe assay were unable to distinguish between these species.},
  articleno    = {104},
  author       = {El Aila, Nabil and Emler, Stefan and Kaijalainen, Tarja and De Baere, Thierry and Saerens, Bart and Alkan, Elife and Deschaght, Pieter and Verhelst, Rita and Vaneechoutte, Mario},
  issn         = {1471-2334},
  journal      = {BMC INFECTIOUS DISEASES},
  language     = {eng},
  pages        = {8},
  title        = {The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniae and comparison with four other species specific PCR assays},
  url          = {http://dx.doi.org/10.1186/1471-2334-10-104},
  volume       = {10},
  year         = {2010},
}

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