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Production, characterization and in vitro testing of HBcAg-specific VHH intrabodies

Benedikte Serruys UGent, Freya Van Houtte UGent, Aliasghar Farhoudi Moghadam UGent, Geert Leroux-Roels UGent and Peter Vanlandschoot (2010) JOURNAL OF GENERAL VIROLOGY. 91(3). p.643-652
abstract
Hepatitis B virus (HBV) infections represent a global health problem, since these account for 350 million chronic infections worldwide that result in 500 000-700 000 deaths each year. Control of viral replication and HBV-related disease and mortality are of utmost importance. Because the currently available antiviral therapies all have major limitations, new strategies to treat chronic HBV infection are eagerly awaited. Six single-domain antibodies (VHHs) targeting the core antigen of HBV (HBcAg) have been generated and three of these bound strongly to HBcAg of both subtype ayw and adw. These three VHHs were studied as intrabodies directed towards the nucleus or the cytoplasm of a hepatoma cell line that was co-transfected with HBV. A speckled staining of HBcAg was observed in the cytoplasm of cells transfected with nucleotropic VHH intrabodies. Moreover, an increased intracellular accumulation of hepatitis B e antigen (HBeAg) and a complete disappearance of intracellular HBcAg signal were observed with nuclear targeted HBcAg-specific VHHs. These results suggest that HBcAg-specific VHHs targeted to the nucleus affect HBcAg and HBeAg expression and trafficking in HBV-transfected hepatocytes.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
REPLICATION, DISEASE, CORE ANTIGEN, INHIBITION, CELLS, INDUCIBLE MXA PROTEIN, PREVENTION, RNA, EXPRESSION, HEPATITIS-B-VIRUS
journal title
JOURNAL OF GENERAL VIROLOGY
J. Gen. Virol.
volume
91
issue
3
pages
643 - 652
Web of Science type
Article
Web of Science id
000275587200007
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.568 (2010)
JCR rank
35/158 (2010)
JCR quartile
1 (2010)
ISSN
0022-1317
DOI
10.1099/vir.0.016063-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1229835
handle
http://hdl.handle.net/1854/LU-1229835
date created
2011-05-23 11:32:22
date last changed
2011-06-06 08:23:10
@article{1229835,
  abstract     = {Hepatitis B virus (HBV) infections represent a global health problem, since these account for 350 million chronic infections worldwide that result in 500 000-700 000 deaths each year. Control of viral replication and HBV-related disease and mortality are of utmost importance. Because the currently available antiviral therapies all have major limitations, new strategies to treat chronic HBV infection are eagerly awaited. Six single-domain antibodies (VHHs) targeting the core antigen of HBV (HBcAg) have been generated and three of these bound strongly to HBcAg of both subtype ayw and adw. These three VHHs were studied as intrabodies directed towards the nucleus or the cytoplasm of a hepatoma cell line that was co-transfected with HBV. A speckled staining of HBcAg was observed in the cytoplasm of cells transfected with nucleotropic VHH intrabodies. Moreover, an increased intracellular accumulation of hepatitis B e antigen (HBeAg) and a complete disappearance of intracellular HBcAg signal were observed with nuclear targeted HBcAg-specific VHHs. These results suggest that HBcAg-specific VHHs targeted to the nucleus affect HBcAg and HBeAg expression and trafficking in HBV-transfected hepatocytes.},
  author       = {Serruys, Benedikte and Van Houtte, Freya and Farhoudi Moghadam, Aliasghar and Leroux-Roels, Geert and Vanlandschoot, Peter},
  issn         = {0022-1317},
  journal      = {JOURNAL OF GENERAL VIROLOGY},
  keyword      = {REPLICATION,DISEASE,CORE ANTIGEN,INHIBITION,CELLS,INDUCIBLE MXA PROTEIN,PREVENTION,RNA,EXPRESSION,HEPATITIS-B-VIRUS},
  language     = {eng},
  number       = {3},
  pages        = {643--652},
  title        = {Production, characterization and in vitro testing of HBcAg-specific VHH intrabodies},
  url          = {http://dx.doi.org/10.1099/vir.0.016063-0},
  volume       = {91},
  year         = {2010},
}

Chicago
Serruys, Benedikte, Freya Van Houtte, Aliasghar Farhoudi Moghadam, Geert Leroux-Roels, and Peter Vanlandschoot. 2010. “Production, Characterization and in Vitro Testing of HBcAg-specific VHH Intrabodies.” Journal of General Virology 91 (3): 643–652.
APA
Serruys, B., Van Houtte, F., Farhoudi Moghadam, A., Leroux-Roels, G., & Vanlandschoot, P. (2010). Production, characterization and in vitro testing of HBcAg-specific VHH intrabodies. JOURNAL OF GENERAL VIROLOGY, 91(3), 643–652.
Vancouver
1.
Serruys B, Van Houtte F, Farhoudi Moghadam A, Leroux-Roels G, Vanlandschoot P. Production, characterization and in vitro testing of HBcAg-specific VHH intrabodies. JOURNAL OF GENERAL VIROLOGY. 2010;91(3):643–52.
MLA
Serruys, Benedikte, Freya Van Houtte, Aliasghar Farhoudi Moghadam, et al. “Production, Characterization and in Vitro Testing of HBcAg-specific VHH Intrabodies.” JOURNAL OF GENERAL VIROLOGY 91.3 (2010): 643–652. Print.