Challenge pools of hepatitis C virus genotypes 1-6 prototype strains: replication fitness and pathogenicity in chimpanzees and human liver-chimeric mouse models
- Author
- Jens Bukh, Philip Meuleman (UGent) , Raymond Tellier, Ronald E Engle, Stephen M Feinstone, Gerald Eder, William C Satterfield, Sugantha Govindarajan, Krzysztof Krawczynski, Roger H Miller, Geert Leroux-Roels (UGent) and Robert H Purcell
- Organization
- Abstract
- Chimpanzees represent the only animal model for studies of the natural history of hepatitis C virus (HCV). To generate virus stocks of important HCV variants, we infected chimpanzees with HCV strains of genotypes 1-6 and determined the infectivity titer of acute-phase plasma pools in additional animals. The courses of first- and second-passage infections were similar, with early appearance of viremia, HCV RNA titers of >10(4.7) IU/mL, and development of acute hepatitis; the chronicity rate was 56%. The challenge pools had titers of 10(3)-10(5) chimpanzee infectious doses/mL. Human liver-chimeric mice developed high-titer infections after inoculation with the challenge viruses of genotypes 1-6. Inoculation studies with different doses of the genotype 1b pool suggested that a relatively high virus dose is required to consistently infect chimeric mice. The challenge pools represent a unique resource for studies of HCV molecular virology and for studies of pathogenesis, protective immunity, and vaccine efficacy in vivo.
- Keywords
- INFECTIOUS IN-VIVO, CELL-CULTURE-SYSTEMS, 6 MAJOR GENOTYPES, UPA-SCID MOUSE, INOCULATED CHIMPANZEES, FULMINANT-HEPATITIS, NUCLEOTIDE-SEQUENCE, MOLECULAR CLONE, CDNA-CLONE, TRANSCRIPTS
Downloads
-
Bukh JID 2010.pdf
- full text
- |
- open access
- |
- |
- 417.36 KB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-1229776
- MLA
- Bukh, Jens, et al. “Challenge Pools of Hepatitis C Virus Genotypes 1-6 Prototype Strains: Replication Fitness and Pathogenicity in Chimpanzees and Human Liver-Chimeric Mouse Models.” JOURNAL OF INFECTIOUS DISEASES, vol. 201, no. 9, 2010, pp. 1381–89, doi:10.1086/651579.
- APA
- Bukh, J., Meuleman, P., Tellier, R., Engle, R. E., Feinstone, S. M., Eder, G., … Purcell, R. H. (2010). Challenge pools of hepatitis C virus genotypes 1-6 prototype strains: replication fitness and pathogenicity in chimpanzees and human liver-chimeric mouse models. JOURNAL OF INFECTIOUS DISEASES, 201(9), 1381–1389. https://doi.org/10.1086/651579
- Chicago author-date
- Bukh, Jens, Philip Meuleman, Raymond Tellier, Ronald E Engle, Stephen M Feinstone, Gerald Eder, William C Satterfield, et al. 2010. “Challenge Pools of Hepatitis C Virus Genotypes 1-6 Prototype Strains: Replication Fitness and Pathogenicity in Chimpanzees and Human Liver-Chimeric Mouse Models.” JOURNAL OF INFECTIOUS DISEASES 201 (9): 1381–89. https://doi.org/10.1086/651579.
- Chicago author-date (all authors)
- Bukh, Jens, Philip Meuleman, Raymond Tellier, Ronald E Engle, Stephen M Feinstone, Gerald Eder, William C Satterfield, Sugantha Govindarajan, Krzysztof Krawczynski, Roger H Miller, Geert Leroux-Roels, and Robert H Purcell. 2010. “Challenge Pools of Hepatitis C Virus Genotypes 1-6 Prototype Strains: Replication Fitness and Pathogenicity in Chimpanzees and Human Liver-Chimeric Mouse Models.” JOURNAL OF INFECTIOUS DISEASES 201 (9): 1381–1389. doi:10.1086/651579.
- Vancouver
- 1.Bukh J, Meuleman P, Tellier R, Engle RE, Feinstone SM, Eder G, et al. Challenge pools of hepatitis C virus genotypes 1-6 prototype strains: replication fitness and pathogenicity in chimpanzees and human liver-chimeric mouse models. JOURNAL OF INFECTIOUS DISEASES. 2010;201(9):1381–9.
- IEEE
- [1]J. Bukh et al., “Challenge pools of hepatitis C virus genotypes 1-6 prototype strains: replication fitness and pathogenicity in chimpanzees and human liver-chimeric mouse models,” JOURNAL OF INFECTIOUS DISEASES, vol. 201, no. 9, pp. 1381–1389, 2010.
@article{1229776,
abstract = {{Chimpanzees represent the only animal model for studies of the natural history of hepatitis C virus (HCV). To generate virus stocks of important HCV variants, we infected chimpanzees with HCV strains of genotypes 1-6 and determined the infectivity titer of acute-phase plasma pools in additional animals. The courses of first- and second-passage infections were similar, with early appearance of viremia, HCV RNA titers of >10(4.7) IU/mL, and development of acute hepatitis; the chronicity rate was 56%. The challenge pools had titers of 10(3)-10(5) chimpanzee infectious doses/mL. Human liver-chimeric mice developed high-titer infections after inoculation with the challenge viruses of genotypes 1-6. Inoculation studies with different doses of the genotype 1b pool suggested that a relatively high virus dose is required to consistently infect chimeric mice. The challenge pools represent a unique resource for studies of HCV molecular virology and for studies of pathogenesis, protective immunity, and vaccine efficacy in vivo.}},
author = {{Bukh, Jens and Meuleman, Philip and Tellier, Raymond and Engle, Ronald E and Feinstone, Stephen M and Eder, Gerald and Satterfield, William C and Govindarajan, Sugantha and Krawczynski, Krzysztof and Miller, Roger H and Leroux-Roels, Geert and Purcell, Robert H}},
issn = {{0022-1899}},
journal = {{JOURNAL OF INFECTIOUS DISEASES}},
keywords = {{INFECTIOUS IN-VIVO,CELL-CULTURE-SYSTEMS,6 MAJOR GENOTYPES,UPA-SCID MOUSE,INOCULATED CHIMPANZEES,FULMINANT-HEPATITIS,NUCLEOTIDE-SEQUENCE,MOLECULAR CLONE,CDNA-CLONE,TRANSCRIPTS}},
language = {{eng}},
number = {{9}},
pages = {{1381--1389}},
title = {{Challenge pools of hepatitis C virus genotypes 1-6 prototype strains: replication fitness and pathogenicity in chimpanzees and human liver-chimeric mouse models}},
url = {{http://doi.org/10.1086/651579}},
volume = {{201}},
year = {{2010}},
}
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: