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A novel technique for the in vivo imaging of autoimmune diabetes development in the pancreas by two-photon microscopy

(2010) PLOS ONE. 5(12).
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Abstract
Type 1 diabetes (T1D) is characterized by the immune-mediated destruction of beta cells in the pancreas. Little is known about the in vivo dynamic interactions between T cells and beta cells or the kinetic behavior of other immune cell subsets in the pancreatic islets. Utilizing multiphoton microscopy we have designed a technique that allows for the real-time visualization of diabetogenic T cells and dendritic cells in pancreatic islets in a live animal, including their interplay with beta cells and the vasculature. Using a custom designed stage, the pancreas was surgically exposed under live conditions so that imaging of islets under intact blood pressure and oxygen supply became possible. We demonstrate here that this approach allows for the tracking of diabetogenic leukocytes as well as vascularization phenotype of islets and accumulation of dendritic cells in islets during diabetes pathogenesis. This technique should be useful in mapping crucial kinetic events in T1D pathogenesis and in testing the impact of immune based interventions on T cell migration, extravasation and islet destruction.
Keywords
DENDRITIC CELLS, TRANSGENIC MICE, IMMUNE-SYSTEM, BETA-CELLS, REAL-TIME, TOLERANCE, ISLETS, ANTIGEN, PROTEIN, MOUSE

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Citation

Please use this url to cite or link to this publication:

Chicago
Coppieters, Ken, Marianne M Martinic, William B Kiosses, Natalie Amirian, and Matthias von Herrath. 2010. “A Novel Technique for the in Vivo Imaging of Autoimmune Diabetes Development in the Pancreas by Two-photon Microscopy.” Plos One 5 (12).
APA
Coppieters, K., Martinic, M. M., Kiosses, W. B., Amirian, N., & von Herrath, M. (2010). A novel technique for the in vivo imaging of autoimmune diabetes development in the pancreas by two-photon microscopy. PLOS ONE, 5(12).
Vancouver
1.
Coppieters K, Martinic MM, Kiosses WB, Amirian N, von Herrath M. A novel technique for the in vivo imaging of autoimmune diabetes development in the pancreas by two-photon microscopy. PLOS ONE. 2010;5(12).
MLA
Coppieters, Ken, Marianne M Martinic, William B Kiosses, et al. “A Novel Technique for the in Vivo Imaging of Autoimmune Diabetes Development in the Pancreas by Two-photon Microscopy.” PLOS ONE 5.12 (2010): n. pag. Print.
@article{1229302,
  abstract     = {Type 1 diabetes (T1D) is characterized by the immune-mediated destruction of beta cells in the pancreas. Little is known about the in vivo dynamic interactions between T cells and beta cells or the kinetic behavior of other immune cell subsets in the pancreatic islets. Utilizing multiphoton microscopy we have designed a technique that allows for the real-time visualization of diabetogenic T cells and dendritic cells in pancreatic islets in a live animal, including their interplay with beta cells and the vasculature. Using a custom designed stage, the pancreas was surgically exposed under live conditions so that imaging of islets under intact blood pressure and oxygen supply became possible. We demonstrate here that this approach allows for the tracking of diabetogenic leukocytes as well as vascularization phenotype of islets and accumulation of dendritic cells in islets during diabetes pathogenesis. This technique should be useful in mapping crucial kinetic events in T1D pathogenesis and in testing the impact of immune based interventions on T cell migration, extravasation and islet destruction.},
  articleno    = {e15732},
  author       = {Coppieters, Ken and Martinic, Marianne M and Kiosses, William B and Amirian, Natalie and von Herrath, Matthias},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {DENDRITIC CELLS,TRANSGENIC MICE,IMMUNE-SYSTEM,BETA-CELLS,REAL-TIME,TOLERANCE,ISLETS,ANTIGEN,PROTEIN,MOUSE},
  language     = {eng},
  number       = {12},
  pages        = {8},
  title        = {A novel technique for the in vivo imaging of autoimmune diabetes development in the pancreas by two-photon microscopy},
  url          = {http://dx.doi.org/10.1371/journal.pone.0015732},
  volume       = {5},
  year         = {2010},
}

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