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Suppression of hippocampal epileptic seizures in the kainate rat by Poisson distributed stimulation

Tine Wyckhuys (UGent) , Paul Boon (UGent) , Robrecht Raedt (UGent) , Bregt Van Nieuwenhuyse (UGent) , Kristl Vonck (UGent) and Wytse Wadman (UGent)
(2010) EPILEPSIA. 51(11). p.2297-2304
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Abstract
Purpose: Hippocampal deep brain stimulation (DBS) is an experimental therapy for patients with pharmacoresistant temporal lobe epilepsy (TLE). Despite the successful clinical application of DBS, the optimal stimulation parameters are undetermined. We evaluate the efficacy of a new form of DBS, using continuous stimuli with Poisson distributed intervals (Poisson distributed stimulation, PDS) in the kainate (KA) rat model, a validated model for human TLE. Methods: Status epilepticus was elicited by injection of KA (i.p.). After development of spontaneous seizures, rats were implanted with hippocampal DBS- and depth electroencephalography (EEG) electrodes. After baseline EEG monitoring, one group of rats (n = 13) was treated with PDS and a second (n = 11) received regular high frequency stimulation (HFS) at 130 Hz. Stimulation intensity was 100 mu A below the threshold for induction of epileptiform EEG activity. Results: Stimulation intensity was significantly lower for PDS (156 +/- 20 mu A) than HFS (207 +/- 23 mu A; p < 0.02). Seven (54%) of 13 rats treated with PDS and 5 (45%) of 11 rats treated with HFS experienced a significant reduction in seizure frequency. In PDS-improved rats, seizure frequency was reduced to 33% (p < 0.01) of baseline value and in HFS-improved rats to 50% (p < 0.01). After termination of PDS, seizure rate returned to baseline value. Discussion: Continuous hippocampal PDS significantly reduces the number of spontaneous seizures. Compared to regular HFS, there is a slightly larger number of improved rats and a larger efficacy at a considerably lower stimulus intensity. The first two observations leave room for optimization, whereas a lower intensity is beneficial for battery life.
Keywords
SPONTANEOUS MOTOR SEIZURES, LONG-TERM POTENTIATION, TEMPORAL-LOBE EPILEPSY, DEEP BRAIN-STIMULATION, Hippocampus, Deep brain stimulation, Temporal lobe epilepsy, ELECTRICAL-STIMULATION, MODEL, PLASTICITY

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Chicago
Wyckhuys, Tine, Paul Boon, Robrecht Raedt, Bregt Van Nieuwenhuyse, Kristl Vonck, and Wytse Wadman. 2010. “Suppression of Hippocampal Epileptic Seizures in the Kainate Rat by Poisson Distributed Stimulation.” Epilepsia 51 (11): 2297–2304.
APA
Wyckhuys, T., Boon, P., Raedt, R., Van Nieuwenhuyse, B., Vonck, K., & Wadman, W. (2010). Suppression of hippocampal epileptic seizures in the kainate rat by Poisson distributed stimulation. EPILEPSIA, 51(11), 2297–2304.
Vancouver
1.
Wyckhuys T, Boon P, Raedt R, Van Nieuwenhuyse B, Vonck K, Wadman W. Suppression of hippocampal epileptic seizures in the kainate rat by Poisson distributed stimulation. EPILEPSIA. 2010;51(11):2297–304.
MLA
Wyckhuys, Tine, Paul Boon, Robrecht Raedt, et al. “Suppression of Hippocampal Epileptic Seizures in the Kainate Rat by Poisson Distributed Stimulation.” EPILEPSIA 51.11 (2010): 2297–2304. Print.
@article{1228380,
  abstract     = {Purpose: Hippocampal deep brain stimulation (DBS) is an experimental therapy for patients with pharmacoresistant temporal lobe epilepsy (TLE). Despite the successful clinical application of DBS, the optimal stimulation parameters are undetermined. We evaluate the efficacy of a new form of DBS, using continuous stimuli with Poisson distributed intervals (Poisson distributed stimulation, PDS) in the kainate (KA) rat model, a validated model for human TLE.
Methods: Status epilepticus was elicited by injection of KA (i.p.). After development of spontaneous seizures, rats were implanted with hippocampal DBS- and depth electroencephalography (EEG) electrodes. After baseline EEG monitoring, one group of rats (n = 13) was treated with PDS and a second (n = 11) received regular high frequency stimulation (HFS) at 130 Hz. Stimulation intensity was 100 mu A below the threshold for induction of epileptiform EEG activity.
Results: Stimulation intensity was significantly lower for PDS (156 +/- 20 mu A) than HFS (207 +/- 23 mu A; p {\textlangle} 0.02). Seven (54\%) of 13 rats treated with PDS and 5 (45\%) of 11 rats treated with HFS experienced a significant reduction in seizure frequency. In PDS-improved rats, seizure frequency was reduced to 33\% (p {\textlangle} 0.01) of baseline value and in HFS-improved rats to 50\% (p {\textlangle} 0.01). After termination of PDS, seizure rate returned to baseline value.
Discussion: Continuous hippocampal PDS significantly reduces the number of spontaneous seizures. Compared to regular HFS, there is a slightly larger number of improved rats and a larger efficacy at a considerably lower stimulus intensity. The first two observations leave room for optimization, whereas a lower intensity is beneficial for battery life.},
  author       = {Wyckhuys, Tine and Boon, Paul and Raedt, Robrecht and Van Nieuwenhuyse, Bregt and Vonck, Kristl and Wadman, Wytse},
  issn         = {0013-9580},
  journal      = {EPILEPSIA},
  keyword      = {SPONTANEOUS MOTOR SEIZURES,LONG-TERM POTENTIATION,TEMPORAL-LOBE EPILEPSY,DEEP BRAIN-STIMULATION,Hippocampus,Deep brain stimulation,Temporal lobe epilepsy,ELECTRICAL-STIMULATION,MODEL,PLASTICITY},
  language     = {eng},
  number       = {11},
  pages        = {2297--2304},
  title        = {Suppression of hippocampal epileptic seizures in the kainate rat by Poisson distributed stimulation},
  url          = {http://dx.doi.org/10.1111/j.1528-1167.2010.02750.x},
  volume       = {51},
  year         = {2010},
}

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