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Electroporation of immature and mature dendritic cells: implications for dendritic cell-based vaccines

A Michiels, S Tuyaerts, Aude Bonehill, J Corthals, Karine Breckpot, Carlo Heirman, Sonja Van Meirvenne, Melissa Dullaers UGent, S Allard, F Brasseur, et al. (2005) GENE THERAPY. 12(9). p.772-782
abstract
Until now, studies utilizing mRNA electroporation as a tool for the delivery of tumor antigens to human monocyte-derived dendritic cells ( DC) have focused on DC electroporated in an immature state. Immature DC are considered to be specialized in antigen capture and processing, whereas mature DC present antigen and have an increased T-cell stimulatory capacity. Therefore, the consensus has been to electroporate DC before maturation. We show that the transfection efficiency of DC electroporated either before or after maturation was similarly high. Both immature and mature electroporated DC, matured in the presence of an inflammatory cytokine cocktail, expressed mature DC surface markers and preserved their capacity to secrete cytokines and chemokines upon CD40 ligation. In addition, both immature and mature DC can be efficiently cryopreserved before or after electroporation without deleterious effects on viability, phenotype or T-cell stimulatory capacity including in vitro antigen-specific T-cell activation. However, DC electroporated after maturation are more efficient in in vitro migration assays and at least as effective in antigen presentation as DC electroporated before maturation. These results are important for vaccination strategies where an optimal antigen presentation by DC after migration to the lymphoid organs is crucial.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
HUMAN BLOOD, INDUCTION, CERVICAL-CANCER, ANTITUMOR IMMUNITY, IN-VITRO, CTL RESPONSES, TUMOR-ANTIGEN, MESSENGER-RNA, CANCER-IMMUNOTHERAPY, CYTOTOXIC T-LYMPHOCYTES, cancer immunotherapy, antigen presentation, dendritic cells, mRNA electroporation
journal title
GENE THERAPY
Gene Ther.
volume
12
issue
9
pages
772 - 782
Web of Science type
Article
Web of Science id
000228553800008
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
4.836 (2005)
JCR rank
14/135 (2005)
JCR quartile
1 (2005)
ISSN
0969-7128
DOI
10.1038/sj.gt.3302471
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1227861
handle
http://hdl.handle.net/1854/LU-1227861
date created
2011-05-20 09:24:53
date last changed
2016-12-19 15:42:50
@article{1227861,
  abstract     = {Until now, studies utilizing mRNA electroporation as a tool for the delivery of tumor antigens to human monocyte-derived dendritic cells ( DC) have focused on DC electroporated in an immature state. Immature DC are considered to be specialized in antigen capture and processing, whereas mature DC present antigen and have an increased T-cell stimulatory capacity. Therefore, the consensus has been to electroporate DC before maturation. We show that the transfection efficiency of DC electroporated either before or after maturation was similarly high. Both immature and mature electroporated DC, matured in the presence of an inflammatory cytokine cocktail, expressed mature DC surface markers and preserved their capacity to secrete cytokines and chemokines upon CD40 ligation. In addition, both immature and mature DC can be efficiently cryopreserved before or after electroporation without deleterious effects on viability, phenotype or T-cell stimulatory capacity including in vitro antigen-specific T-cell activation. However, DC electroporated after maturation are more efficient in in vitro migration assays and at least as effective in antigen presentation as DC electroporated before maturation. These results are important for vaccination strategies where an optimal antigen presentation by DC after migration to the lymphoid organs is crucial.},
  author       = {Michiels, A and Tuyaerts, S and Bonehill, Aude and Corthals, J and Breckpot, Karine and Heirman, Carlo and Van Meirvenne, Sonja and Dullaers, Melissa and Allard, S and Brasseur, F and van der Bruggen, Pierre and Thielemans, Kris},
  issn         = {0969-7128},
  journal      = {GENE THERAPY},
  keyword      = {HUMAN BLOOD,INDUCTION,CERVICAL-CANCER,ANTITUMOR IMMUNITY,IN-VITRO,CTL RESPONSES,TUMOR-ANTIGEN,MESSENGER-RNA,CANCER-IMMUNOTHERAPY,CYTOTOXIC T-LYMPHOCYTES,cancer immunotherapy,antigen presentation,dendritic cells,mRNA electroporation},
  language     = {eng},
  number       = {9},
  pages        = {772--782},
  title        = {Electroporation of immature and mature dendritic cells: implications for dendritic cell-based vaccines},
  url          = {http://dx.doi.org/10.1038/sj.gt.3302471},
  volume       = {12},
  year         = {2005},
}

Chicago
Michiels, A, S Tuyaerts, Aude Bonehill, J Corthals, Karine Breckpot, Carlo Heirman, Sonja Van Meirvenne, et al. 2005. “Electroporation of Immature and Mature Dendritic Cells: Implications for Dendritic Cell-based Vaccines.” Gene Therapy 12 (9): 772–782.
APA
Michiels, A, Tuyaerts, S., Bonehill, A., Corthals, J., Breckpot, K., Heirman, C., Van Meirvenne, S., et al. (2005). Electroporation of immature and mature dendritic cells: implications for dendritic cell-based vaccines. GENE THERAPY, 12(9), 772–782.
Vancouver
1.
Michiels A, Tuyaerts S, Bonehill A, Corthals J, Breckpot K, Heirman C, et al. Electroporation of immature and mature dendritic cells: implications for dendritic cell-based vaccines. GENE THERAPY. 2005;12(9):772–82.
MLA
Michiels, A, S Tuyaerts, Aude Bonehill, et al. “Electroporation of Immature and Mature Dendritic Cells: Implications for Dendritic Cell-based Vaccines.” GENE THERAPY 12.9 (2005): 772–782. Print.