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Stress-induced survival strategies enable Salmonella Enteritidis to persistently colonize the chicken oviduct tissue and cope with antimicrobial factors in egg white: a hypothesis to explain a pandemic

Filip Van Immerseel UGent (2010) GUT PATHOGENS. 2(1).
abstract
Background: Egg-associated transmission to humans seems to be characteristic of the Salmonella serotype Enteritidis, explaining why this particular serotype has caused a worldwide pandemic since the mid '80s. Salmonella Enteritidis is much more capable to persistently colonize the laying hen reproductive tract and to survive in the hostile egg white, as compared to other serotypes. Presentation of the hypothesis: It is hypothesized that stress-induced survival mechanisms enable the serotype Enteritidis to persistently colonize the oviduct without causing damage and excessive inflammation, and to cope with the antimicrobial compounds present in egg white. Testing the hypothesis: To test the hypothesis first of all Salmonella Enteritidis genes that are essential for colonization of the oviduct and survival in eggs need to be identified. Comparative genomics tools should be used to identify genes or pathogenicity islands that are present in Salmonella Enteritidis and not in the multiple non egg-contaminating serotypes. High-throughput signature-tagged-mutagenesis approaches, coupled to micro-array detection of the genes that lead to an attenuated phenotype when mutated is proposed as an ideal tool to identify genes involved in oviduct colonization and egg white survival. Identifying the stressors and antibacterial molecules in the oviduct and in the egg white that limit colonization or survival of non-Enteritidis serotypes is a second important objective that can theoretically be achieved using screenings of expressed oviduct cDNA libraries for their antibacterial activity against strains from multiple serotypes. Finally, the effect of contact with these stressors in the oviduct or egg white on Salmonella gene expression will need to be analyzed, in order to clarify whether serotype Enteritidis-specific regulation of certain stress-survival pathways are either or not present. Implications of the hypothesis: Knowledge on the pathogenesis of egg infections would furthermore give insights that might be extrapolated to other biological interactions, in which a highly specialized bacterial pathogen resists the host response in a specific biological niche. In addition, this info can be of value in developing early warning criteria to identify emerging egg-associated Salmonella strains and in developing safe live attenuated vaccine strains.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
GUT PATHOGENS
Gut Pathog.
volume
2
issue
1
article number
23
pages
5 pages
Web of Science type
Article
Web of Science id
000208167500023
ISSN
1757-4749
DOI
10.1186/1757-4749-2-23
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
1225974
handle
http://hdl.handle.net/1854/LU-1225974
date created
2011-05-18 10:52:37
date last changed
2016-12-21 15:42:24
@article{1225974,
  abstract     = {Background: Egg-associated transmission to humans seems to be characteristic of the Salmonella serotype Enteritidis, explaining why this particular serotype has caused a worldwide pandemic since the mid '80s. Salmonella Enteritidis is much more capable to persistently colonize the laying hen reproductive tract and to survive in the hostile egg white, as compared to other serotypes. 
Presentation of the hypothesis: It is hypothesized that stress-induced survival mechanisms enable the serotype Enteritidis to persistently colonize the oviduct without causing damage and excessive inflammation, and to cope with the antimicrobial compounds present in egg white. 
Testing the hypothesis: To test the hypothesis first of all Salmonella Enteritidis genes that are essential for colonization of the oviduct and survival in eggs need to be identified. Comparative genomics tools should be used to identify genes or pathogenicity islands that are present in Salmonella Enteritidis and not in the multiple non egg-contaminating serotypes. High-throughput signature-tagged-mutagenesis approaches, coupled to micro-array detection of the genes that lead to an attenuated phenotype when mutated is proposed as an ideal tool to identify genes involved in oviduct colonization and egg white survival. Identifying the stressors and antibacterial molecules in the oviduct and in the egg white that limit colonization or survival of non-Enteritidis serotypes is a second important objective that can theoretically be achieved using screenings of expressed oviduct cDNA libraries for their antibacterial activity against strains from multiple serotypes. Finally, the effect of contact with these stressors in the oviduct or egg white on Salmonella gene expression will need to be analyzed, in order to clarify whether serotype Enteritidis-specific regulation of certain stress-survival pathways are either or not present. 
Implications of the hypothesis: Knowledge on the pathogenesis of egg infections would furthermore give insights that might be extrapolated to other biological interactions, in which a highly specialized bacterial pathogen resists the host response in a specific biological niche. In addition, this info can be of value in developing early warning criteria to identify emerging egg-associated Salmonella strains and in developing safe live attenuated vaccine strains.},
  articleno    = {23},
  author       = {Van Immerseel, Filip},
  issn         = {1757-4749},
  journal      = {GUT PATHOGENS},
  language     = {eng},
  number       = {1},
  pages        = {5},
  title        = {Stress-induced survival strategies enable Salmonella Enteritidis to persistently colonize the chicken oviduct tissue and cope with antimicrobial factors in egg white: a hypothesis to explain a pandemic},
  url          = {http://dx.doi.org/10.1186/1757-4749-2-23},
  volume       = {2},
  year         = {2010},
}

Chicago
Van Immerseel, Filip. 2010. “Stress-induced Survival Strategies Enable Salmonella Enteritidis to Persistently Colonize the Chicken Oviduct Tissue and Cope with Antimicrobial Factors in Egg White: a Hypothesis to Explain a Pandemic.” Gut Pathogens 2 (1).
APA
Van Immerseel, Filip. (2010). Stress-induced survival strategies enable Salmonella Enteritidis to persistently colonize the chicken oviduct tissue and cope with antimicrobial factors in egg white: a hypothesis to explain a pandemic. GUT PATHOGENS, 2(1).
Vancouver
1.
Van Immerseel F. Stress-induced survival strategies enable Salmonella Enteritidis to persistently colonize the chicken oviduct tissue and cope with antimicrobial factors in egg white: a hypothesis to explain a pandemic. GUT PATHOGENS. 2010;2(1).
MLA
Van Immerseel, Filip. “Stress-induced Survival Strategies Enable Salmonella Enteritidis to Persistently Colonize the Chicken Oviduct Tissue and Cope with Antimicrobial Factors in Egg White: a Hypothesis to Explain a Pandemic.” GUT PATHOGENS 2.1 (2010): n. pag. Print.