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Isolation of disseminated neuroblastoma cells from bone marrow aspirates for pretreatment risk assessment by array comparative genomic hybridization

Mado Vandewoestyne UGent, Candy Kumps UGent, Katrien Swerts UGent, Björn Menten UGent, Tim Lammens UGent, Jan Philippé UGent, Katleen De Preter UGent, Genevieve Laureys UGent, Nadine Van Roy UGent and Franki Speleman UGent, et al. (2012) INTERNATIONAL JOURNAL OF CANCER. 130(5). p.1098-1108
abstract
In neuroblastoma, tumor biopsies are used for prognostic evaluation and risk assessment by molecular genetic analyses such as fluorescence in situ hybridization (FISH) and array comparative genomic hybridization (array CGH). Analysis of primary tumors by array CGH can be hampered by the lack of sufficient tumor cells due to small biopsy size or availability of invaded bone marrow only. Given the importance of accurate assessment of genetic alterations in the diagnostic work-up of patients with neuroblastoma, we evaluated the possibility to analyze bone marrow metastases in patients with disseminated disease. Disseminated neuroblastoma cells were isolated from bone marrow aspirates by using either laser capture microdissection (LCM) or magnetic activated cell sorting (MACS). The array CGH profiles of these isolated metastases were compared to array CGH profiles and/or FISH data of the corresponding primary tumor. Here, we show that the major recurrent DNA copy number alterations detected in primary neuroblastoma tumors (i.e., 1p, 3p and 11q deletion, 17q gain and MYCN amplification) can be detected, with high sensitivity and specificity, in the disseminated neuroblastoma cells isolated from the bone marrow aspirates, using an array platform with high coverage for these regions. Moreover, we demonstrate that for archived material, for example, for retrospective studies, LCM is the method of choice, while for fresh bone marrow aspirates, acquired at the time of diagnosis, MACS is superior.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
dissemination, neuroblastoma, pre-treatment risk assessment, array comparative genomic hybridization, laser capture microdissection, magnetic activated cell sorting, LASER CAPTURE MICRODISSECTION, ACTIVATING MUTATIONS, ALK KINASE, BIOLOGY, TUMORS, AMPLIFICATION, CGH, REARRANGEMENTS, METASTASES, DELETION
journal title
INTERNATIONAL JOURNAL OF CANCER
Int. J. Cancer
volume
130
issue
5
pages
1098 - 1108
Web of Science type
Article
Web of Science id
000298601500012
JCR category
ONCOLOGY
JCR impact factor
6.198 (2012)
JCR rank
23/196 (2012)
JCR quartile
1 (2012)
ISSN
0020-7136
DOI
10.1002/ijc.26133
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1224752
handle
http://hdl.handle.net/1854/LU-1224752
date created
2011-05-16 15:41:36
date last changed
2012-04-25 13:02:52
@article{1224752,
  abstract     = {In neuroblastoma, tumor biopsies are used for prognostic evaluation and risk assessment by molecular genetic analyses such as fluorescence in situ hybridization (FISH) and array comparative genomic hybridization (array CGH). Analysis of primary tumors by array CGH can be hampered by the lack of sufficient tumor cells due to small biopsy size or availability of invaded bone marrow only. Given the importance of accurate assessment of genetic alterations in the diagnostic work-up of patients with neuroblastoma, we evaluated the possibility to analyze bone marrow metastases in patients with disseminated disease. Disseminated neuroblastoma cells were isolated from bone marrow aspirates by using either laser capture microdissection (LCM) or magnetic activated cell sorting (MACS). The array CGH profiles of these isolated metastases were compared to array CGH profiles and/or FISH data of the corresponding primary tumor. Here, we show that the major recurrent DNA copy number alterations detected in primary neuroblastoma tumors (i.e., 1p, 3p and 11q deletion, 17q gain and MYCN amplification) can be detected, with high sensitivity and specificity, in the disseminated neuroblastoma cells isolated from the bone marrow aspirates, using an array platform with high coverage for these regions. Moreover, we demonstrate that for archived material, for example, for retrospective studies, LCM is the method of choice, while for fresh bone marrow aspirates, acquired at the time of diagnosis, MACS is superior.},
  author       = {Vandewoestyne, Mado and Kumps, Candy and Swerts, Katrien and Menten, Bj{\"o}rn and Lammens, Tim and Philipp{\'e}, Jan and De Preter, Katleen and Laureys, Genevieve and Van Roy, Nadine and Speleman, Franki and Deforce, Dieter},
  issn         = {0020-7136},
  journal      = {INTERNATIONAL JOURNAL OF CANCER},
  keyword      = {dissemination,neuroblastoma,pre-treatment risk assessment,array comparative genomic hybridization,laser capture microdissection,magnetic activated cell sorting,LASER CAPTURE MICRODISSECTION,ACTIVATING MUTATIONS,ALK KINASE,BIOLOGY,TUMORS,AMPLIFICATION,CGH,REARRANGEMENTS,METASTASES,DELETION},
  language     = {eng},
  number       = {5},
  pages        = {1098--1108},
  title        = {Isolation of disseminated neuroblastoma cells from bone marrow aspirates for pretreatment risk assessment by array comparative genomic hybridization},
  url          = {http://dx.doi.org/10.1002/ijc.26133},
  volume       = {130},
  year         = {2012},
}

Chicago
Vandewoestyne, Mado, Candy Kumps, Katrien Swerts, Björn Menten, Tim Lammens, Jan Philippé, Katleen De Preter, et al. 2012. “Isolation of Disseminated Neuroblastoma Cells from Bone Marrow Aspirates for Pretreatment Risk Assessment by Array Comparative Genomic Hybridization.” International Journal of Cancer 130 (5): 1098–1108.
APA
Vandewoestyne, M., Kumps, C., Swerts, K., Menten, B., Lammens, T., Philippé, J., De Preter, K., et al. (2012). Isolation of disseminated neuroblastoma cells from bone marrow aspirates for pretreatment risk assessment by array comparative genomic hybridization. INTERNATIONAL JOURNAL OF CANCER, 130(5), 1098–1108.
Vancouver
1.
Vandewoestyne M, Kumps C, Swerts K, Menten B, Lammens T, Philippé J, et al. Isolation of disseminated neuroblastoma cells from bone marrow aspirates for pretreatment risk assessment by array comparative genomic hybridization. INTERNATIONAL JOURNAL OF CANCER. 2012;130(5):1098–108.
MLA
Vandewoestyne, Mado, Candy Kumps, Katrien Swerts, et al. “Isolation of Disseminated Neuroblastoma Cells from Bone Marrow Aspirates for Pretreatment Risk Assessment by Array Comparative Genomic Hybridization.” INTERNATIONAL JOURNAL OF CANCER 130.5 (2012): 1098–1108. Print.