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Expression of virulence genes in luminescent and nonluminescent isogenic vibrios and virulence towards gnotobiotic brine shrimp (Artemia franciscana)

Darshanee Ruwandeepika Hettipala Arachchige UGent, Tom Defoirdt UGent, P P Bhowmick, I Karunasagar and Peter Bossier UGent (2011) JOURNAL OF APPLIED MICROBIOLOGY. 110(2). p.399-406
abstract
Aims: This study aimed to evaluate the expression levels of virulence gene regulators (luxR and toxR) and virulence factors (serine protease, metalloprotease and haemolysin) in luminescent and nonluminescent isogenic Vibrio harveyi and Vibrio campbellii. Method and Results: Nonluminescent variants have been reported before to become dominant in cultures of luminescent vibrios when grown under static conditions in the dark. Wild-type V. harveyi BB120, V. campbellii LMG 21363, quorum sensing mutants of V. harveyi BB120 and their previously reported nonluminescent isogenic counterparts were used in this study. The expression level of the virulence genes srp serine protease, vhp metalloprotease and vhh haemolysin, the quorum sensing master regulator gene luxR and the virulence regulator gene toxR in isogenic luminescent and nonluminescent strains were quantified using reverse transcriptase real-time PCR. These experiments revealed that the nonluminescent strains produced lower levels of the quorum sensing master regulator gene luxR and the vhp metalloprotease gene (which is known to be regulated by quorum sensing). Finally, challenge tests with gnotobiotic brine shrimp (Artemia franciscana) larvae revealed that the nonluminescent strains are less virulent than their luminescent isogenic counterparts. Conclusion: Nonluminescent variants of V. harveyi and V. campbellii strains produce lower levels of the quorum sensing master regulator gene luxR and the vhp metalloprotease gene and are less virulent to brine shrimp than their isogenic luminescent counterparts. Significance and Impact of the study: These results indicate that adaptation of luminescent vibrios to specific growth conditions that result in a dominant nonluminescent phenotype is accompanied by a decreased adaptation to a host environment because of altered virulence gene regulation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SEQUENCE, gene expression, AUTOINDUCERS, COMMUNICATION, BACTERIA, PATHOGEN, HARVEYI, QUORUM-SENSING SYSTEMS, quorum sensing, virulence
journal title
JOURNAL OF APPLIED MICROBIOLOGY
J. Appl. Microbiol.
volume
110
issue
2
pages
399 - 406
Web of Science type
Article
Web of Science id
000285972900003
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
2.337 (2011)
JCR rank
71/157 (2011)
JCR quartile
2 (2011)
ISSN
1364-5072
DOI
10.1111/j.1365-2672.2010.04892.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1224286
handle
http://hdl.handle.net/1854/LU-1224286
date created
2011-05-16 13:24:07
date last changed
2016-12-19 15:46:38
@article{1224286,
  abstract     = {Aims: This study aimed to evaluate the expression levels of virulence gene regulators (luxR and toxR) and virulence factors (serine protease, metalloprotease and haemolysin) in luminescent and nonluminescent isogenic Vibrio harveyi and Vibrio campbellii. Method and Results: Nonluminescent variants have been reported before to become dominant in cultures of luminescent vibrios when grown under static conditions in the dark. Wild-type V. harveyi BB120, V. campbellii LMG 21363, quorum sensing mutants of V. harveyi BB120 and their previously reported nonluminescent isogenic counterparts were used in this study. The expression level of the virulence genes srp serine protease, vhp metalloprotease and vhh haemolysin, the quorum sensing master regulator gene luxR and the virulence regulator gene toxR in isogenic luminescent and nonluminescent strains were quantified using reverse transcriptase real-time PCR. These experiments revealed that the nonluminescent strains produced lower levels of the quorum sensing master regulator gene luxR and the vhp metalloprotease gene (which is known to be regulated by quorum sensing). Finally, challenge tests with gnotobiotic brine shrimp (Artemia franciscana) larvae revealed that the nonluminescent strains are less virulent than their luminescent isogenic counterparts. Conclusion: Nonluminescent variants of V. harveyi and V. campbellii strains produce lower levels of the quorum sensing master regulator gene luxR and the vhp metalloprotease gene and are less virulent to brine shrimp than their isogenic luminescent counterparts. Significance and Impact of the study: These results indicate that adaptation of luminescent vibrios to specific growth conditions that result in a dominant nonluminescent phenotype is accompanied by a decreased adaptation to a host environment because of altered virulence gene regulation.},
  author       = {Ruwandeepika Hettipala Arachchige, Darshanee and Defoirdt, Tom and Bhowmick, P P and Karunasagar, I and Bossier, Peter},
  issn         = {1364-5072},
  journal      = {JOURNAL OF APPLIED MICROBIOLOGY},
  keyword      = {SEQUENCE,gene expression,AUTOINDUCERS,COMMUNICATION,BACTERIA,PATHOGEN,HARVEYI,QUORUM-SENSING SYSTEMS,quorum sensing,virulence},
  language     = {eng},
  number       = {2},
  pages        = {399--406},
  title        = {Expression of virulence genes in luminescent and nonluminescent isogenic vibrios and virulence towards gnotobiotic brine shrimp (Artemia franciscana)},
  url          = {http://dx.doi.org/10.1111/j.1365-2672.2010.04892.x},
  volume       = {110},
  year         = {2011},
}

Chicago
Ruwandeepika Hettipala Arachchige, Darshanee, Tom Defoirdt, P P Bhowmick, I Karunasagar, and Peter Bossier. 2011. “Expression of Virulence Genes in Luminescent and Nonluminescent Isogenic Vibrios and Virulence Towards Gnotobiotic Brine Shrimp (Artemia Franciscana).” Journal of Applied Microbiology 110 (2): 399–406.
APA
Ruwandeepika Hettipala Arachchige, D., Defoirdt, T., Bhowmick, P. P., Karunasagar, I., & Bossier, P. (2011). Expression of virulence genes in luminescent and nonluminescent isogenic vibrios and virulence towards gnotobiotic brine shrimp (Artemia franciscana). JOURNAL OF APPLIED MICROBIOLOGY, 110(2), 399–406.
Vancouver
1.
Ruwandeepika Hettipala Arachchige D, Defoirdt T, Bhowmick PP, Karunasagar I, Bossier P. Expression of virulence genes in luminescent and nonluminescent isogenic vibrios and virulence towards gnotobiotic brine shrimp (Artemia franciscana). JOURNAL OF APPLIED MICROBIOLOGY. 2011;110(2):399–406.
MLA
Ruwandeepika Hettipala Arachchige, Darshanee, Tom Defoirdt, P P Bhowmick, et al. “Expression of Virulence Genes in Luminescent and Nonluminescent Isogenic Vibrios and Virulence Towards Gnotobiotic Brine Shrimp (Artemia Franciscana).” JOURNAL OF APPLIED MICROBIOLOGY 110.2 (2011): 399–406. Print.