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Development of analytical strategies using U-HPLC-MS/MS and LC-ToF-MS for the quantification of micropollutants in marine organisms

Klaas Wille (UGent) , Julie Kiebooms (UGent) , Michiel Claessens (UGent) , Karen Rappé (UGent) , Julie Vanden Bussche (UGent) , Herlinde Noppe (UGent) , Nander Van Praet, Eric De Wulf, Peter Van Caeter (UGent) , Colin Janssen (UGent) , et al.
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Abstract
Organic micropollutants such as pharmaceuticals, perfluorinated compounds (PFCs), and pesticides, are important environmental contaminants. To obtain more information regarding their presence in marine organisms, an increasing demand exists for reliable analytical methods for quantification of these micropollutants in biotic matrices. Therefore, we developed extraction procedures and new analytical methods for the quantification of 14 pesticides, 10 PFCs, and 11 pharmaceuticals in tissue of marine organisms, namely blue mussels (Mytilus edulis). This paper presents these optimized analytical procedures and their application to M. edulis, deployed at five stations in the Belgian coastal zone. The methods consisted of a pressurized liquid extraction and solid-phase extraction (SPE) followed by ultra high-performance liquid chromatography coupled to triple quadrupole mass spectrometry for pharmaceuticals and pesticides, and of a liquid extraction using acetonitrile and SPE, followed by liquid chromatography coupled to time-of-flight mass spectrometry for PFCs. The limits of quantification of the three newly optimized analytical procedures in M. edulis tissue varied between 0.1 and 10 ng g(-1), and satisfactory linearities (a parts per thousand yen0.98) and recoveries (90-106%) were obtained. Application of these methods to M. edulis revealed the presence of five pharmaceuticals, two PFCs, and seven pesticides at levels up to 490, 5, and 60 ng g(-1), respectively. The most prevalent micropollutants were salicylic acid, paracetamol, perfluorooctane sulfonate, chloridazon, and dichlorvos.
Keywords
AQUATIC ENVIRONMENT, SCHELDT ESTUARY, PERFLUOROALKYLATED SUBSTANCES, PERFLUORINATED COMPOUNDS, WASTE-WATER, LIQUID-CHROMATOGRAPHY, PERSONAL CARE PRODUCTS, WATER TREATMENT PLANTS, SOLID-PHASE EXTRACTION, TANDEM MASS-SPECTROMETRY, Pharmaceuticals, Perfluorinated compounds, Pesticides, Marine organisms, Liquid chromatography, Mass spectrometry

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Chicago
Wille, Klaas, Julie Kiebooms, Michiel Claessens, Karen Rappé, Julie Vanden Bussche, Herlinde Noppe, Nander Van Praet, et al. 2011. “Development of Analytical Strategies Using U-HPLC-MS/MS and LC-ToF-MS for the Quantification of Micropollutants in Marine Organisms.” Analytical and Bioanalytical Chemistry 400 (5): 1459–1472.
APA
Wille, K., Kiebooms, J., Claessens, M., Rappé, K., Vanden Bussche, J., Noppe, H., Van Praet, N., et al. (2011). Development of analytical strategies using U-HPLC-MS/MS and LC-ToF-MS for the quantification of micropollutants in marine organisms. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 400(5), 1459–1472.
Vancouver
1.
Wille K, Kiebooms J, Claessens M, Rappé K, Vanden Bussche J, Noppe H, et al. Development of analytical strategies using U-HPLC-MS/MS and LC-ToF-MS for the quantification of micropollutants in marine organisms. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2011;400(5):1459–72.
MLA
Wille, Klaas, Julie Kiebooms, Michiel Claessens, et al. “Development of Analytical Strategies Using U-HPLC-MS/MS and LC-ToF-MS for the Quantification of Micropollutants in Marine Organisms.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 400.5 (2011): 1459–1472. Print.
@article{1224113,
  abstract     = {Organic micropollutants such as pharmaceuticals, perfluorinated compounds (PFCs), and pesticides, are important environmental contaminants. To obtain more information regarding their presence in marine organisms, an increasing demand exists for reliable analytical methods for quantification of these micropollutants in biotic matrices. Therefore, we developed extraction procedures and new analytical methods for the quantification of 14 pesticides, 10 PFCs, and 11 pharmaceuticals in tissue of marine organisms, namely blue mussels (Mytilus edulis). This paper presents these optimized analytical procedures and their application to M. edulis, deployed at five stations in the Belgian coastal zone. The methods consisted of a pressurized liquid extraction and solid-phase extraction (SPE) followed by ultra high-performance liquid chromatography coupled to triple quadrupole mass spectrometry for pharmaceuticals and pesticides, and of a liquid extraction using acetonitrile and SPE, followed by liquid chromatography coupled to time-of-flight mass spectrometry for PFCs. The limits of quantification of the three newly optimized analytical procedures in M. edulis tissue varied between 0.1 and 10 ng g(-1), and satisfactory linearities (a parts per thousand yen0.98) and recoveries (90-106\%) were obtained. Application of these methods to M. edulis revealed the presence of five pharmaceuticals, two PFCs, and seven pesticides at levels up to 490, 5, and 60 ng g(-1), respectively. The most prevalent micropollutants were salicylic acid, paracetamol, perfluorooctane sulfonate, chloridazon, and dichlorvos.},
  author       = {Wille, Klaas and Kiebooms, Julie and Claessens, Michiel and Rapp{\'e}, Karen and Vanden Bussche, Julie and Noppe, Herlinde and Van Praet, Nander and De Wulf, Eric and Van Caeter, Peter and Janssen, Colin and De Brabander, Hubert and Vanhaecke, Lynn},
  issn         = {1618-2642},
  journal      = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
  keyword      = {AQUATIC ENVIRONMENT,SCHELDT ESTUARY,PERFLUOROALKYLATED SUBSTANCES,PERFLUORINATED COMPOUNDS,WASTE-WATER,LIQUID-CHROMATOGRAPHY,PERSONAL CARE PRODUCTS,WATER TREATMENT PLANTS,SOLID-PHASE EXTRACTION,TANDEM MASS-SPECTROMETRY,Pharmaceuticals,Perfluorinated compounds,Pesticides,Marine organisms,Liquid chromatography,Mass spectrometry},
  language     = {eng},
  number       = {5},
  pages        = {1459--1472},
  title        = {Development of analytical strategies using U-HPLC-MS/MS and LC-ToF-MS for the quantification of micropollutants in marine organisms},
  url          = {http://dx.doi.org/10.1007/s00216-011-4878-6},
  volume       = {400},
  year         = {2011},
}

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