Advanced search
1 file | 3.55 MB

Diversity and detection of microfungi and their mycotoxins in silage

Els Van Pamel (UGent)
(2011)
Author
Promoter
Annemieke Verbeken and Els Daeseleire
Organization
Abstract
Ensiling maize represents a very important agricultural activity leading to prolonged preservation of the crop. However, when the (anaerobic and low pH) conditions necessary for obtaining maize silage of an excellent quality are disrupted, mould growth can occur. The presence of moulds will lead to a reduction of the nutritional value of this feed commodity, but can also lead to the production of secondary metabolites or mycotoxins. These toxins can be produced during post-harvest stages as well as in the field (pre-harvest). Mycotoxins can cause both profound and hidden symptoms influencing growth, production, fertility, digestion, the immune system and other. As a result, they can negatively influence animal production/welfare/health. Silage commodities are mostly contaminated with several mycotoxins, resulting in a possible increased (synergetic) health risk. The presence of moulds and mycotoxins in silage commodities has important economic implications due to for example feed ration loss, decreased animal production and veterinary costs. In some cases, certain of these toxins or their metabolites can be transmitted into animal-derived products such as meat and milk. This research focused on the development of a methodology for the detection, identification and quantification of moulds and mycotoxins contaminating maize silage. Within this framework, finding a suitable medium for isolating and enumerating fungal contaminants in silage was aimed at. Because conventional plating techniques are often time-consuming and expertise-demanding, more objective, genotype-based methods were also used for molecularly identifying and characterizing the mould species in different Belgian silage samples. As information on the mycotoxin production of a fungal isolate can be used as an additional confirmation of its identity, an Ultra High Performance Liquid Chromatography-tandem mass spectrometric (UHPLC-MS/MS) method was developed for the simultaneous detection of over twenty mycotoxins from Yeast Extract Sucrose (YES) agar, a growth medium suitable for inducing secondary metabolite production by fungal isolates. Finally, an UHPLC-MS/MS method for the simultaneous detection of 27 mycotoxins from maize silage was developed and validated. In conclusion, combining different techniques available for mould and mycotoxin detection/identification, i.e., a multidisciplinary or polyphasic research approach, will result in more reliable results which are important in view of protecting animal health/welfare.
Keywords
mycotoxins, moulds, maize silage

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 3.55 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Van Pamel, Els. 2011. “Diversity and Detection of Microfungi and Their Mycotoxins in Silage”. Ghent, Belgium: Ghent University. Faculty of Sciences.
APA
Van Pamel, E. (2011). Diversity and detection of microfungi and their mycotoxins in silage. Ghent University. Faculty of Sciences, Ghent, Belgium.
Vancouver
1.
Van Pamel E. Diversity and detection of microfungi and their mycotoxins in silage. [Ghent, Belgium]: Ghent University. Faculty of Sciences; 2011.
MLA
Van Pamel, Els. “Diversity and Detection of Microfungi and Their Mycotoxins in Silage.” 2011 : n. pag. Print.
@phdthesis{1223739,
  abstract     = {Ensiling maize represents a very important agricultural activity leading to prolonged preservation of the crop. However, when the (anaerobic and low pH) conditions necessary for obtaining maize silage of an excellent quality are disrupted, mould growth can occur. The presence of moulds will lead to a reduction of the nutritional value of this feed commodity, but can also lead to the production of secondary metabolites or mycotoxins. These toxins can be produced during post-harvest stages as well as in the field (pre-harvest). Mycotoxins can cause both profound and hidden symptoms influencing growth, production, fertility, digestion, the immune system and other. As a result, they can negatively influence animal production/welfare/health. Silage commodities are mostly contaminated with several mycotoxins, resulting in a possible increased (synergetic) health risk. The presence of moulds and mycotoxins in silage commodities has important economic implications due to for example feed ration loss, decreased animal production and veterinary costs. In some cases, certain of these toxins or their metabolites can be transmitted into animal-derived products such as meat and milk.
This research focused on the development of a methodology for the detection, identification and quantification of moulds and mycotoxins contaminating maize silage. Within this framework, finding a suitable medium for isolating and enumerating fungal contaminants in silage was aimed at. Because conventional plating techniques are often time-consuming and expertise-demanding, more objective, genotype-based methods were also used for molecularly identifying and characterizing the mould species in different Belgian silage samples. As information on the mycotoxin production of a fungal isolate can be used as an additional confirmation of its identity, an Ultra High Performance Liquid Chromatography-tandem mass spectrometric (UHPLC-MS/MS) method was developed for the simultaneous detection of over twenty mycotoxins from Yeast Extract Sucrose (YES) agar, a growth medium suitable for inducing secondary metabolite production by fungal isolates. Finally, an UHPLC-MS/MS method for the simultaneous detection of 27 mycotoxins from maize silage was developed and validated. In conclusion, combining different techniques available for mould and mycotoxin detection/identification, i.e., a multidisciplinary or polyphasic research approach, will result in more reliable results which are important in view of protecting animal health/welfare.},
  author       = {Van Pamel, Els},
  isbn         = {9789490695651},
  keyword      = {mycotoxins,moulds,maize silage},
  language     = {eng},
  pages        = {202},
  publisher    = {Ghent University. Faculty of Sciences},
  school       = {Ghent University},
  title        = {Diversity and detection of microfungi and their mycotoxins in silage},
  year         = {2011},
}