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Culturable diversity of heterotrophic bacteria in Forlidas Pond (Pensacola Mountains) and Lundström Lake (Shackleton Range), Antarctica

Karolien Peeters UGent, Dominic A Hodgson, Peter Convey and Anne Willems UGent (2011) MICROBIAL ECOLOGY. 62(2). p.399-413
abstract
Cultivation techniques were used to study the heterotrophic bacterial diversity in two microbial mat samples originating from the littoral zone of two continental Antarctic lakes (Forlidas Pond and Lundström Lake) in the Dufek Massif (within the Pensacola Mountains group of the Transantarctic Mountains) and Shackleton Range, respectively. Nearly 800 isolates were picked after incubation on several growth media at different temperatures. They were grouped using a whole-genome fingerprinting technique, repetitive element palindromic-PCR and partial 16S rRNA gene sequencing. Phylogenetic analysis of the complete 16S rRNA gene sequences of 82 representatives showed that the isolates belonged to four major phylogenetic groups: Actinobacteria, Bacteroidetes, Proteobacteria and Firmicutes. A relatively large difference between the samples was apparent. Forlidas Pond is a completely frozen water body underlain by a hypersaline brine, with summer thaw forming a slightly saline littoral moat. This was reflected in the bacterial diversity with a dominance of isolates belonging to Firmicutes, whereas isolates from the freshwater Lundström Lake revealed a dominance of Actinobacteria. A total of 42 different genera were recovered, including first records from Antarctica for Albidiferax, Bosea, Curvibacter, Luteimonas, Ornithinibacillus, Pseudoxanthomonas, Sphingopyxis and Spirosoma. Additionally, a considerable number of potential new species and new genera were recovered distributed over different phylogenetic groups. For several species where previously only the type strain was available in cultivation, we report additional strains. Comparison with public databases showed that 72% of the phylotypes were cosmopolitan whereas 23% were apparently currently restricted to Antarctica. Furthermore, the majority of the Bacteroidetes phylotypes were restricted to Antarctica and an important part of these represent previously unknown species.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GEN. NOV., EMENDED DESCRIPTION, SP-NOV., ET-AL. 2003, CYANOBACTERIAL MAT SAMPLE, MCMURDO DRY VALLEYS, cyanobacterial mat, 16S rRNA gene sequencing, cultivation, ASPA., Transantarctic Mountains, SEA-ICE, PSYCHROPHILIC BACTERIA, SPECIES DEFINITION, MELTWATER PONDS
journal title
MICROBIAL ECOLOGY
Microb. Ecol.
volume
62
issue
2
pages
399 - 413
Web of Science type
Article
Web of Science id
000293964000014
JCR category
MARINE & FRESHWATER BIOLOGY
JCR impact factor
2.912 (2011)
JCR rank
9/97 (2011)
JCR quartile
1 (2011)
ISSN
0095-3628
DOI
10.1007/s00248-011-9842-7
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1220529
handle
http://hdl.handle.net/1854/LU-1220529
date created
2011-05-10 11:04:57
date last changed
2012-05-02 15:39:44
@article{1220529,
  abstract     = {Cultivation techniques were used to study the heterotrophic bacterial diversity in two microbial mat samples originating from the littoral zone of two continental Antarctic lakes (Forlidas Pond and Lundstr{\"o}m Lake) in the Dufek Massif (within the Pensacola Mountains group of the Transantarctic Mountains) and Shackleton Range, respectively. Nearly 800 isolates were picked after incubation on several growth media at different temperatures. They were grouped using a whole-genome fingerprinting technique, repetitive element palindromic-PCR and partial 16S rRNA gene sequencing. Phylogenetic analysis of the complete 16S rRNA gene sequences of 82 representatives showed that the isolates belonged to four major phylogenetic groups: Actinobacteria, Bacteroidetes, Proteobacteria and Firmicutes. A relatively large difference between the samples was apparent. Forlidas Pond is a completely frozen water body underlain by a hypersaline brine, with summer thaw forming a slightly saline littoral moat. This was reflected in the bacterial diversity with a dominance of isolates belonging to Firmicutes, whereas isolates from the freshwater Lundstr{\"o}m Lake revealed a dominance of Actinobacteria. A total of 42 different genera were recovered, including first records from Antarctica for Albidiferax, Bosea, Curvibacter, Luteimonas, Ornithinibacillus, Pseudoxanthomonas, Sphingopyxis and Spirosoma. Additionally, a considerable number of potential new species and new genera were recovered distributed over different phylogenetic groups. For several species where previously only the type strain was available in cultivation, we report additional strains.  Comparison with public databases showed that 72\% of the phylotypes were cosmopolitan whereas 23\% were apparently currently restricted to Antarctica. Furthermore, the majority of the Bacteroidetes phylotypes were restricted to Antarctica and an important part of these represent previously unknown species.},
  author       = {Peeters, Karolien and Hodgson, Dominic A and Convey, Peter and Willems, Anne},
  issn         = {0095-3628},
  journal      = {MICROBIAL ECOLOGY},
  keyword      = {GEN. NOV.,EMENDED DESCRIPTION,SP-NOV.,ET-AL. 2003,CYANOBACTERIAL MAT SAMPLE,MCMURDO DRY VALLEYS,cyanobacterial mat,16S rRNA gene sequencing,cultivation,ASPA.,Transantarctic Mountains,SEA-ICE,PSYCHROPHILIC BACTERIA,SPECIES DEFINITION,MELTWATER PONDS},
  language     = {eng},
  number       = {2},
  pages        = {399--413},
  title        = {Culturable diversity of heterotrophic bacteria in Forlidas Pond (Pensacola Mountains) and Lundstr{\"o}m Lake (Shackleton Range), Antarctica},
  url          = {http://dx.doi.org/10.1007/s00248-011-9842-7},
  volume       = {62},
  year         = {2011},
}

Chicago
Peeters, Karolien, Dominic A Hodgson, Peter Convey, and Anne Willems. 2011. “Culturable Diversity of Heterotrophic Bacteria in Forlidas Pond (Pensacola Mountains) and Lundström Lake (Shackleton Range), Antarctica.” Microbial Ecology 62 (2): 399–413.
APA
Peeters, Karolien, Hodgson, D. A., Convey, P., & Willems, A. (2011). Culturable diversity of heterotrophic bacteria in Forlidas Pond (Pensacola Mountains) and Lundström Lake (Shackleton Range), Antarctica. MICROBIAL ECOLOGY, 62(2), 399–413.
Vancouver
1.
Peeters K, Hodgson DA, Convey P, Willems A. Culturable diversity of heterotrophic bacteria in Forlidas Pond (Pensacola Mountains) and Lundström Lake (Shackleton Range), Antarctica. MICROBIAL ECOLOGY. 2011;62(2):399–413.
MLA
Peeters, Karolien, Dominic A Hodgson, Peter Convey, et al. “Culturable Diversity of Heterotrophic Bacteria in Forlidas Pond (Pensacola Mountains) and Lundström Lake (Shackleton Range), Antarctica.” MICROBIAL ECOLOGY 62.2 (2011): 399–413. Print.