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Identification of the UDP-glucosyltransferase gene UGTA1, responsible for the first glucosylation step in the sophorolipid biosynthetic pathway of candida bombicola ATCC 22214

Karen Saerens (UGent) , Sophie Roelants (UGent) , Inge Van Bogaert (UGent) and Wim Soetaert (UGent)
(2011) FEMS YEAST RESEARCH. 11(1). p.123-132
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Candida bombicola ATCC 22214 is applied commercially for the production of sophorolipids from renewable resources such as vegetable oils or waste streams. Although much research has been performed on optimization of fermentation conditions and on the influence of feed source and process parameters on sophorolipid structures and yields, the metabolic pathway of these important bioproducts remains unclear. Here, we identify a glucosyltransferase gene UGTA1 and show that the gene product is responsible for the first glucosylation step in the biosynthetic pathway of sophorolipids. Moreover, we provide evidence that the second glucosylation step is catalysed by a different glucosyltransferase that acts independently from the first. Therefore, the biosynthesis of sophorolipids by C. bombicola involves two glucosyltransferases that act in a stepwise manner. The UGTA1 gene described here is the first identified gene with a clear function in sophorolipid production by this economically important yeast.
Keywords
sophorolipid, glucosyltransferase, Candida bombicola, biosurfactant, RHAMNOLIPID BIOSURFACTANT SYNTHESIS, PSEUDOMONAS-AERUGINOSA, DECARBOXYLASE GENE, USTILAGO-MAYDIS, RAPESEED OIL, YEAST, GLYCOSYLTRANSFERASES, BOGORIENSIS, ACID, CLASSIFICATION

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Citation

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Chicago
Saerens, Karen, Sophie Roelants, Inge Van Bogaert, and Wim Soetaert. 2011. “Identification of the UDP-glucosyltransferase Gene UGTA1, Responsible for the First Glucosylation Step in the Sophorolipid Biosynthetic Pathway of Candida Bombicola ATCC 22214.” Fems Yeast Research 11 (1): 123–132.
APA
Saerens, K., Roelants, S., Van Bogaert, I., & Soetaert, W. (2011). Identification of the UDP-glucosyltransferase gene UGTA1, responsible for the first glucosylation step in the sophorolipid biosynthetic pathway of candida bombicola ATCC 22214. FEMS YEAST RESEARCH, 11(1), 123–132.
Vancouver
1.
Saerens K, Roelants S, Van Bogaert I, Soetaert W. Identification of the UDP-glucosyltransferase gene UGTA1, responsible for the first glucosylation step in the sophorolipid biosynthetic pathway of candida bombicola ATCC 22214. FEMS YEAST RESEARCH. 2011;11(1):123–32.
MLA
Saerens, Karen, Sophie Roelants, Inge Van Bogaert, et al. “Identification of the UDP-glucosyltransferase Gene UGTA1, Responsible for the First Glucosylation Step in the Sophorolipid Biosynthetic Pathway of Candida Bombicola ATCC 22214.” FEMS YEAST RESEARCH 11.1 (2011): 123–132. Print.
@article{1219719,
  abstract     = {Candida bombicola ATCC 22214 is applied commercially for the production of sophorolipids from renewable resources such as vegetable oils or waste streams. Although much research has been performed on optimization of fermentation conditions and on the influence of feed source and process parameters on sophorolipid structures and yields, the metabolic pathway of these important bioproducts remains unclear. Here, we identify a glucosyltransferase gene UGTA1 and show that the gene product is responsible for the first glucosylation step in the biosynthetic pathway of sophorolipids. Moreover, we provide evidence that the second glucosylation step is catalysed by a different glucosyltransferase that acts independently from the first. Therefore, the biosynthesis of sophorolipids by C. bombicola involves two glucosyltransferases that act in a stepwise manner. The UGTA1 gene described here is the first identified gene with a clear function in sophorolipid production by this economically important yeast.},
  author       = {Saerens, Karen and Roelants, Sophie and Van Bogaert, Inge and Soetaert, Wim},
  issn         = {1567-1356},
  journal      = {FEMS YEAST RESEARCH},
  keyword      = {sophorolipid,glucosyltransferase,Candida bombicola,biosurfactant,RHAMNOLIPID BIOSURFACTANT SYNTHESIS,PSEUDOMONAS-AERUGINOSA,DECARBOXYLASE GENE,USTILAGO-MAYDIS,RAPESEED OIL,YEAST,GLYCOSYLTRANSFERASES,BOGORIENSIS,ACID,CLASSIFICATION},
  language     = {eng},
  number       = {1},
  pages        = {123--132},
  title        = {Identification of the UDP-glucosyltransferase gene UGTA1, responsible for the first glucosylation step in the sophorolipid biosynthetic pathway of candida bombicola ATCC 22214},
  url          = {http://dx.doi.org/10.1111/j.1567-1364.2010.00695.x},
  volume       = {11},
  year         = {2011},
}

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