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Efficacy of an autogenous vaccine against highly virulent Staphylococcus aureus infection in rabbits

Godelieve Meulemans UGent, Freddy Haesebrouck UGent, Urszula Lipinska, Luc Duchateau UGent and Katleen Hermans UGent (2011) WORLD RABBIT SCIENCE. 19(1). p.1-9
abstract
The efficacy of an autogenous vaccine consisting of a whole cell suspension of formalin killed bacteria in sterile buffered saline against Staphylococcus aureus infections was determined, using a well-established rabbit skin infection model. Thirteen 8 wk old rabbits were vaccinated twice subcutaneously with a 2 wk interval while 10 rabbits were injected twice with formalised PBS (sterile phosphate buffered saline). Two weeks after the last injection, 10 vaccinated and all PBS-injected rabbits were inoculated intradermally with 10(8) cfu of a S. aureus strain (KH 171)which had been shown to be highly virulent for rabbits. Three vaccinated animals served as negative controls and were intradermally injected with sterile buffered saline. All rabbits were examined daily for the development of skin lesions until 14 d after the experimental infection when all rabbits were euthanised. All animals experimentally infected with S. aureus developed skin abscesses within 24 h post-inoculation, but in the vaccinated group the maximum abscess diameter was significantly lower than in the non-vaccinated group (P=0.048). This difference between autovaccinated and non-vaccinated groups increased over time (P < 0.001). These results indicate that vaccination with an inactivated whole cell bacterin may be useful for control of staphylococcosis in rabbits but does not prevent abscess formation in animals inoculated with a high dose of a highly virulent S. aureus strain.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
FIELD TRIALS, BOVINE MASTITIS, STRAINS, BACTERIN, COLONIZATION, Staphylococcus aureus, virulence, infection, rabbit, skin, autovaccine
journal title
WORLD RABBIT SCIENCE
World Rabbit Sci.
volume
19
issue
1
pages
1 - 9
Web of Science type
Article
Web of Science id
000288878300001
JCR category
AGRICULTURE, DAIRY & ANIMAL SCIENCE
JCR impact factor
0.698 (2011)
JCR rank
28/54 (2011)
JCR quartile
3 (2011)
ISSN
1257-5011
DOI
10.4995/wrs.2011.812
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1218239
handle
http://hdl.handle.net/1854/LU-1218239
date created
2011-05-05 16:06:32
date last changed
2016-12-19 15:45:26
@article{1218239,
  abstract     = {The efficacy of an autogenous vaccine consisting of a whole cell suspension of formalin killed bacteria in sterile buffered saline against Staphylococcus aureus infections was determined, using a well-established rabbit skin infection model. Thirteen 8 wk old rabbits were vaccinated twice subcutaneously with a 2 wk interval while 10 rabbits were injected twice with formalised PBS (sterile phosphate buffered saline). Two weeks after the last injection, 10 vaccinated and all PBS-injected rabbits were inoculated intradermally with 10(8) cfu of a S. aureus strain (KH 171)which had been shown to be highly virulent for rabbits. Three vaccinated animals served as negative controls and were intradermally injected with sterile buffered saline. All rabbits were examined daily for the development of skin lesions until 14 d after the experimental infection when all rabbits were euthanised. All animals experimentally infected with S. aureus developed skin abscesses within 24 h post-inoculation, but in the vaccinated group the maximum abscess diameter was significantly lower than in the non-vaccinated group (P=0.048). This difference between autovaccinated and non-vaccinated groups increased over time (P {\textlangle} 0.001). These results indicate that vaccination with an inactivated whole cell bacterin may be useful for control of staphylococcosis in rabbits but does not prevent abscess formation in animals inoculated with a high dose of a highly virulent S. aureus strain.},
  author       = {Meulemans, Godelieve and Haesebrouck, Freddy and Lipinska, Urszula and Duchateau, Luc and Hermans, Katleen},
  issn         = {1257-5011},
  journal      = {WORLD RABBIT SCIENCE},
  keyword      = {FIELD TRIALS,BOVINE MASTITIS,STRAINS,BACTERIN,COLONIZATION,Staphylococcus aureus,virulence,infection,rabbit,skin,autovaccine},
  language     = {eng},
  number       = {1},
  pages        = {1--9},
  title        = {Efficacy of an autogenous vaccine against highly virulent Staphylococcus aureus infection in rabbits},
  url          = {http://dx.doi.org/10.4995/wrs.2011.812},
  volume       = {19},
  year         = {2011},
}

Chicago
Meulemans, Godelieve, Freddy Haesebrouck, Urszula Lipinska, Luc Duchateau, and Katleen Hermans. 2011. “Efficacy of an Autogenous Vaccine Against Highly Virulent Staphylococcus Aureus Infection in Rabbits.” World Rabbit Science 19 (1): 1–9.
APA
Meulemans, G., Haesebrouck, F., Lipinska, U., Duchateau, L., & Hermans, K. (2011). Efficacy of an autogenous vaccine against highly virulent Staphylococcus aureus infection in rabbits. WORLD RABBIT SCIENCE, 19(1), 1–9.
Vancouver
1.
Meulemans G, Haesebrouck F, Lipinska U, Duchateau L, Hermans K. Efficacy of an autogenous vaccine against highly virulent Staphylococcus aureus infection in rabbits. WORLD RABBIT SCIENCE. 2011;19(1):1–9.
MLA
Meulemans, Godelieve, Freddy Haesebrouck, Urszula Lipinska, et al. “Efficacy of an Autogenous Vaccine Against Highly Virulent Staphylococcus Aureus Infection in Rabbits.” WORLD RABBIT SCIENCE 19.1 (2011): 1–9. Print.