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Presence of helicobacter suis in the oral secretions of slaughtered pigs

Annemieke Smet, Frank Pasmans UGent, Kim Van Deun UGent, Bram Flahou, Richard Ducatelle UGent and Freddy Haesebrouck UGent (2010) 9th international workshop on pathogenesis and host response in helicobacter infections, Abstracts.
abstract
The main Helicobacter species colonizing the stomach of pigs is H. suis which causes gastritis in experimentally and naturally infected pigs. This bacterium has also been associated with ulcers of the nonglandular part of the porcine stomach and is of zoonotic significance. The definite mode of transmission of H. suis between pigs and from pigs to humans remains to be investigated. This study was carried out to determine if the oral secretions of pigs might be a possible route of transmission. Oral swabs were taken from slaughtered pigs originating from 4 different herds (10 pigs/herd). DNA was extracted from these swabs with PrepMan sample preparation reagent from Applied Biosystems as described by the manufacturer. The presence of H. suis DNA was examined using a quantitative PCR based on the urease A gene. In each herd, 2-3 of the 10 swabs were positive for H. suis DNA. These positive samples contained 3000 to 60 000 bacteria. Further studies are required to determine viability of these bacteria.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
in
9th international workshop on pathogenesis and host response in helicobacter infections, Abstracts
conference name
9th International Workshop on Pathogenesis and Host Response in Helicobacter Infections
conference location
Helsingor, Denmark
conference start
2010-07-07
conference end
2010-07-10
language
English
UGent publication?
yes
classification
C3
copyright statement
I have retained and own the full copyright for this publication
id
1217553
handle
http://hdl.handle.net/1854/LU-1217553
date created
2011-05-05 11:47:00
date last changed
2016-12-19 15:35:09
@inproceedings{1217553,
  abstract     = {The main Helicobacter species colonizing the stomach of pigs is H. suis which causes gastritis in experimentally and naturally infected pigs. This bacterium has also been associated with ulcers of the nonglandular part of the porcine stomach and is of zoonotic significance. The definite mode of transmission of H. suis between pigs and from pigs to humans remains to be investigated. This study was carried out to determine if the oral secretions of pigs might be a possible route of transmission. Oral swabs were taken from slaughtered pigs originating from 4 different herds (10 pigs/herd). DNA was extracted from these swabs with PrepMan sample preparation reagent from Applied Biosystems as described by the manufacturer. The presence of H. suis DNA was examined using a quantitative PCR based on the urease A gene. In each herd, 2-3 of the 10 swabs were positive for H. suis DNA. These positive samples contained 3000 to 60 000 bacteria. Further studies are required to determine viability of these bacteria.},
  author       = {Smet, Annemieke and Pasmans, Frank and Van Deun, Kim and Flahou, Bram and Ducatelle, Richard and Haesebrouck, Freddy},
  booktitle    = {9th international workshop on pathogenesis and host response in helicobacter infections, Abstracts},
  language     = {eng},
  location     = {Helsingor, Denmark},
  title        = {Presence of helicobacter suis in the oral secretions of slaughtered pigs},
  year         = {2010},
}

Chicago
Smet, Annemieke, Frank Pasmans, Kim Van Deun, Bram Flahou, Richard Ducatelle, and Freddy Haesebrouck. 2010. “Presence of Helicobacter Suis in the Oral Secretions of Slaughtered Pigs.” In 9th International Workshop on Pathogenesis and Host Response in Helicobacter Infections, Abstracts.
APA
Smet, Annemieke, Pasmans, F., Van Deun, K., Flahou, B., Ducatelle, R., & Haesebrouck, F. (2010). Presence of helicobacter suis in the oral secretions of slaughtered pigs. 9th international workshop on pathogenesis and host response in helicobacter infections, Abstracts. Presented at the 9th International Workshop on Pathogenesis and Host Response in Helicobacter Infections.
Vancouver
1.
Smet A, Pasmans F, Van Deun K, Flahou B, Ducatelle R, Haesebrouck F. Presence of helicobacter suis in the oral secretions of slaughtered pigs. 9th international workshop on pathogenesis and host response in helicobacter infections, Abstracts. 2010.
MLA
Smet, Annemieke, Frank Pasmans, Kim Van Deun, et al. “Presence of Helicobacter Suis in the Oral Secretions of Slaughtered Pigs.” 9th International Workshop on Pathogenesis and Host Response in Helicobacter Infections, Abstracts. 2010. Print.